FLT3, CPTAC-2792 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
FLT3	CD135, FLK2, STK1

Sequence Length (AA)	Molecular Weight (Da)
993	112903

Protein Name
Receptor-type tyrosine-protein kinase FLT3

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MPALARDGGQ	LPLLVVFSAM	IFGTITNQDL	PVIKCVLINH	KNNDSSVGKS
60	70	80	90	100
SSYPMVSESP	EDLGCALRPQ	SSGTVYEAAA	VEVDVSASIT	LQVLVDAPGN
110	120	130	140	150
ISCLWVFKHS	SLNCQPHFDL	QNRGVVSMVI	LKMTETQAGE	YLLFIQSEAT
160	170	180	190	200
NYTILFTVSI	RNTLLYTLRR	PYFRKMENQD	ALVCISESVP	EPIVEWVLCD
210	220	230	240	250
SQGESCKEES	PAVVKKEEKV	LHELFGTDIR	CCARNELGRE	CTRLFTIDLN
260	270	280	290	300
QTPQTTLPQL	FLKVGEPLWI	RCKAVHVNHG	FGLTWELENK	ALEEGNYFEM
310	320	330	340	350
STYSTNRTMI	RILFAFVSSV	ARNDTGYYTC	SSSKHPSQSA	LVTIVEKGFI
360	370	380	390	400
NATNSSEDYE	IDQYEEFCFS	VRFKAYPQIR	CTWTFSRKSF	PCEQKGLDNG
410	420	430	440	450
YSISKFCNHK	HQPGEYIFHA	ENDDAQFTKM	FTLNIRRKPQ	VLAEASASQA
460	470	480	490	500
SCFSDGYPLP	SWTWKKCSDK	SPNCTEEITE	GVWNRKANRK	VFGQWVSSST
510	520	530	540	550
LNMSEAIKGF	LVKCCAYNSL	GTSCETILLN	SPGPFPFIQD	NISFYATIGV
560	570	580	590	600
CLLFIVVLTL	LICHKYKKQF	RYESQLQMVQ	VTGSSDNEYF	YVDFREYEYD
610	620	630	640	650
LKWEFPRENL	EFGKVLGSGA	FGKVMNATAY	GISKTGVSIQ	VAVKMLKEKA
660	670	680	690	700
DSSEREALMS	ELKMMTQLGS	HENIVNLLGA	CTLSGPIYLI	FEYCCYGDLL
710	720	730	740	750
NYLRSKREKF	HRTWTEIFKE	HNFSFYPTFQ	SHPNSSMPGS	REVQIHPDSD
760	770	780	790	800
QISGLHGNSF	HSEDEIEYEN	QKRLEEEEDL	NVLTFEDLLC	FAYQVAKGME
810	820	830	840	850
FLEFKSCVHR	DLAARNVLVT	HGKVVKICDF	GLARDIMSDS	NYVVRGNARL
860	870	880	890	900
PVKWMAPESL	FEGIYTIKSD	VWSYGILLWE	IFSLGVNPYP	GIPVDANFYK
910	920	930	940	950
LIQNGFKMDQ	PFYATEEIYI	IMQSCWAFDS	RKRPSFPNLT	SFLGCQLADA
960	970	980	990	993
EEAMYQNVDG	RVSECPHTYQ	NRRPFSREMD	LGLLSPQAQV	EDS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-2792 Collapse assay details

Data source: Panorama

Official Gene Symbol: FLT3
Peptide Modified Sequence: VSEC[+57.0]PHTY[+80.0]QNR
Modification Type: Carbamidomethyl (C), Phospho (Y)
Protein - Site of Modification: N/A
Peptide - Site of Modification: 4, 8
Peptide Start: 962
Peptide End: 972

CPTAC ID: CPTAC-2792
Peptide Molecular Mass: 1,469.5759
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Mammalian Cell Lysate
Submitting Laboratory: Johns Hopkins University / Pandey
Submitting Lab PI: Akhilesh Pandey

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Orbitrap Fusion Lumos
Internal Standard: 100fmol
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Easy nLC 1200 System
Column Packing: C18 2um 100A
Column Dimensions: 75um i.d. x 50cm
Flow Rate: 250nl/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	40	22	12.8	44.6	41.6	18.8	59.9	47.1	22.7	15	15	15
y6 (1+)	40.4	27	13.9	44.5	48.2	20.2	60.1	55.2	24.5	15	15	15
y5 (1+)	41.5	29.8	15.5	50	54.5	21.2	65	62.1	26.3	15	15	15
y4 (1+)	38	28.5	16.9	44.8	58	24.7	58.7	64.6	29.9	15	15	15
y3 (1+)	38.5	30.1	21.4	59.9	64.9	27.1	71.2	71.5	34.5	15	15	15
b3 (1+)	44	27.6	22.5	61.7	60	31	75.8	66	38.3	15	15	15
sum	38.9	25	14.2	45	48.3	20.7	59.5	54.4	25.1	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for CPTAC-2823 Collapse assay details

Data source: Panorama

Official Gene Symbol: FLT3
Peptide Sequence: GLDNGYSISK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 396
Peptide End: 405

CPTAC ID: CPTAC-2823
Peptide Molecular Mass: 1,052.5138
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend