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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

FLT3

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
FLT3 CD135, FLK2, STK1
Sequence Length (AA) Molecular Weight (Da)
993 112903
Protein Name
Receptor-type tyrosine-protein kinase FLT3
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MPALARDGGQ LPLLVVFSAM IFGTITNQDL PVIKCVLINH KNNDSSVGKS
60 70 80 90 100
SSYPMVSESP EDLGCALRPQ SSGTVYEAAA VEVDVSASIT LQVLVDAPGN
110 120 130 140 150
ISCLWVFKHS SLNCQPHFDL QNRGVVSMVI LKMTETQAGE YLLFIQSEAT
160 170 180 190 200
NYTILFTVSI RNTLLYTLRR PYFRKMENQD ALVCISESVP EPIVEWVLCD
210 220 230 240 250
SQGESCKEES PAVVKKEEKV LHELFGTDIR CCARNELGRE CTRLFTIDLN
260 270 280 290 300
QTPQTTLPQL FLKVGEPLWI RCKAVHVNHG FGLTWELENK ALEEGNYFEM
310 320 330 340 350
STYSTNRTMI RILFAFVSSV ARNDTGYYTC SSSKHPSQSA LVTIVEKGFI
360 370 380 390 400
NATNSSEDYE IDQYEEFCFS VRFKAYPQIR CTWTFSRKSF PCEQKGLDNG
410 420 430 440 450
YSISKFCNHK HQPGEYIFHA ENDDAQFTKM FTLNIRRKPQ VLAEASASQA
460 470 480 490 500
SCFSDGYPLP SWTWKKCSDK SPNCTEEITE GVWNRKANRK VFGQWVSSST
510 520 530 540 550
LNMSEAIKGF LVKCCAYNSL GTSCETILLN SPGPFPFIQD NISFYATIGV
560 570 580 590 600
CLLFIVVLTL LICHKYKKQF RYESQLQMVQ VTGSSDNEYF YVDFREYEYD
610 620 630 640 650
LKWEFPRENL EFGKVLGSGA FGKVMNATAY GISKTGVSIQ VAVKMLKEKA
660 670 680 690 700
DSSEREALMS ELKMMTQLGS HENIVNLLGA CTLSGPIYLI FEYCCYGDLL
710 720 730 740 750
NYLRSKREKF HRTWTEIFKE HNFSFYPTFQ SHPNSSMPGS REVQIHPDSD
760 770 780 790 800
QISGLHGNSF HSEDEIEYEN QKRLEEEEDL NVLTFEDLLC FAYQVAKGME
810 820 830 840 850
FLEFKSCVHR DLAARNVLVT HGKVVKICDF GLARDIMSDS NYVVRGNARL
860 870 880 890 900
PVKWMAPESL FEGIYTIKSD VWSYGILLWE IFSLGVNPYP GIPVDANFYK
910 920 930 940 950
LIQNGFKMDQ PFYATEEIYI IMQSCWAFDS RKRPSFPNLT SFLGCQLADA
960 970 980 990 993
EEAMYQNVDG RVSECPHTYQ NRRPFSREMD LGLLSPQAQV EDS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-2792 Collapse assay details

Data source: Panorama

Official Gene Symbol
FLT3
Peptide Modified Sequence
VSEC[+57.0]PHTY[+80.0]QNR
Modification Type
Carbamidomethyl (C), Phospho (Y)
Protein - Site of Modification
N/A
Peptide - Site of Modification
4, 8
Peptide Start
962
Peptide End
972
CPTAC ID
CPTAC-2792
Peptide Molecular Mass
1,469.5759
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Mammalian Cell Lysate
Submitting Laboratory
Johns Hopkins University / Pandey
Submitting Lab PI
Akhilesh Pandey

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Orbitrap Fusion Lumos
Internal Standard
100fmol
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy nLC 1200 System
Column Packing
C18 2um 100A
Column Dimensions
75um i.d. x 50cm
Flow Rate
250nl/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 40 22 12.8 44.6 41.6 18.8 59.9 47.1 22.7 15 15 15
y6 (1+) 40.4 27 13.9 44.5 48.2 20.2 60.1 55.2 24.5 15 15 15
y5 (1+) 41.5 29.8 15.5 50 54.5 21.2 65 62.1 26.3 15 15 15
y4 (1+) 38 28.5 16.9 44.8 58 24.7 58.7 64.6 29.9 15 15 15
y3 (1+) 38.5 30.1 21.4 59.9 64.9 27.1 71.2 71.5 34.5 15 15 15
b3 (1+) 44 27.6 22.5 61.7 60 31 75.8 66 38.3 15 15 15
sum 38.9 25 14.2 45 48.3 20.7 59.5 54.4 25.1 15 15 15

Additional Resources and Comments

Resources
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Assay Details for CPTAC-2823 Collapse assay details

Data source: Panorama

Official Gene Symbol
FLT3
Peptide Sequence
GLDNGYSISK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
396
Peptide End
405
CPTAC ID
CPTAC-2823
Peptide Molecular Mass
1,052.5138
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive™
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
EASY-nLC™ 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 13.9 6.4 6.7 61.9 7.6 9.3 63.4 9.9 11.5 15 15 15
y4 (1+) 6.7 4.1 6.3 5.8 6.4 5.8 8.9 7.6 8.6 15 15 15
b3 (1+) 15.1 3.7 6.8 13.4 8 5.5 20.2 8.8 8.7 15 15 15
sum 5.2 3.6 4.9 13.5 6.4 5.5 14.5 7.3 7.4 15 15 15

Additional Resources and Comments

Resources
Download SOPs
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Comments

Assay Details for CPTAC-3122 Collapse assay details

Data source: Panorama

Official Gene Symbol
FLT3
Peptide Sequence
HPSQSALVTIVEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
335
Peptide End
347
CPTAC ID
CPTAC-3122
Peptide Molecular Mass
1,407.7722
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 7.8 4.9 7.3 12.1 5.5 7.7 14.4 7.4 10.6 15 15 15
b6 (1+) 7.2 5.2 7.2 10.4 5.3 7.2 12.6 7.4 10.2 15 15 15
y3 (1+) 7.9 4.5 8.4 11.8 5.6 9.1 14.2 7.2 12.4 15 15 15
sum 7.3 4.4 7.4 11.2 4.9 7.3 13.4 6.6 10.4 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
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Comments

Assay Details for CPTAC-3121 Collapse assay details

Data source: Panorama

Official Gene Symbol
FLT3
Peptide Sequence
VLHELFGTDIR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
220
Peptide End
230
CPTAC ID
CPTAC-3121
Peptide Molecular Mass
1,298.6983
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b4 (1+) 6.2 6.7 2.7 15 7.3 5.8 16.2 9.9 6.4 15 15 15
b3 (1+) 9 6.1 3.7 13 7.5 7.6 15.8 9.7 8.5 15 15 15
y5 (1+) 7.8 6.5 3.2 15.9 7.7 7 17.7 10.1 7.7 15 15 15
sum 6.5 6.3 2.7 14.6 7.4 6.5 16 9.7 7 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments