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Overview Data source: UniProt

Official Gene Symbol Other Aliases
GDI2 RABGDIB
Sequence Length (AA) Molecular Weight (Da)
445 50663
Protein Name
Rab GDP dissociation inhibitor beta
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MNEEYDVIVL GTGLTECILS GIMSVNGKKV LHMDRNPYYG GESASITPLE
60 70 80 90 100
DLYKRFKIPG SPPESMGRGR DWNVDLIPKF LMANGQLVKM LLYTEVTRYL
110 120 130 140 150
DFKVTEGSFV YKGGKIYKVP STEAEALASS LMGLFEKRRF RKFLVYVANF
160 170 180 190 200
DEKDPRTFEG IDPKKTTMRD VYKKFDLGQD VIDFTGHALA LYRTDDYLDQ
210 220 230 240 250
PCYETINRIK LYSESLARYG KSPYLYPLYG LGELPQGFAR LSAIYGGTYM
260 270 280 290 300
LNKPIEEIIV QNGKVIGVKS EGEIARCKQL ICDPSYVKDR VEKVGQVIRV
310 320 330 340 350
ICILSHPIKN TNDANSCQII IPQNQVNRKS DIYVCMISFA HNVAAQGKYI
360 370 380 390 400
AIVSTTVETK EPEKEIRPAL ELLEPIEQKF VSISDLLVPK DLGTESQIFI
410 420 430 440 445
SRTYDATTHF ETTCDDIKNI YKRMTGSEFD FEEMKRKKND IYGED

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-512 Collapse assay details

Data source: Panorama

Official Gene Symbol
GDI2
Peptide Modified Sequence
TDDYLDQPC[+57.0]YETINR
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
202
Peptide - Site of Modification
9
Peptide Start
194
Peptide End
208
CPTAC ID
CPTAC-512
Peptide Molecular Mass
1,901.8102
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Seoul National University / Korea Institute of Science and Technology and FHCRC
Submitting Lab PI
Youngsoo Kim

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 22 10 5.4 22.9 11.1 7.2 31.8 14.9 9 15 25 15
y8 (1+) 12.9 6.6 7.9 16 8.2 8.7 20.6 10.5 11.8 15 25 15
y4 (1+) 37.5 8.5 10.4 36.3 10.2 11.9 52.2 13.3 15.8 15 25 15
sum 13 5.8 7.4 12.4 7.9 8.3 18 9.8 11.1 15 25 15

Additional Resources and Comments


Assay Details for CPTAC-513 Collapse assay details

Data source: Panorama

Official Gene Symbol
GDI2
Peptide Sequence
YIAIVSTTVETK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
349
Peptide End
360
CPTAC ID
CPTAC-513
Peptide Molecular Mass
1,323.7286
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Seoul National University / Korea Institute of Science and Technology and FHCRC
Submitting Lab PI
Youngsoo Kim

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 46 17.4 10.3 48.8 21.7 14.3 67.1 27.8 17.6 15 25 15
y8 (1+) 42.9 23.2 15.1 42.5 30.2 14.3 60.4 38.1 20.8 15 25 15
y7 (1+) 62.5 23.7 8.4 94 33.3 9.1 112.9 40.9 12.4 15 25 15
sum 36.5 9 6.5 53.4 14.2 8.7 64.7 16.8 10.9 15 25 15

Additional Resources and Comments


Assay Details for non-CPTAC-3811 Collapse assay details

Data source: Panorama

Official Gene Symbol
Gdi2
Peptide Sequence
DLGTDSQIFISR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
391
Peptide End
402
CPTAC ID
non-CPTAC-3811
Peptide Molecular Mass
1,350.6779
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 10.4 9.9 8 11.2 12.7 9.9 15.3 16.1 12.7 15 15 15
y4 (2+) 111.6 87.6 28.8 85.6 101.9 39.1 140.6 134.4 48.6 15 15 15
y4 (1+) 9.2 7 6.6 9.8 10 10.3 13.4 12.2 12.2 15 15 15
y8 (1+) 10.9 9.7 7.3 15.2 11.4 9.6 18.7 15 12.1 15 15 15
y10 (1+) 8.9 6.5 5 12.2 9.1 11 15.1 11.2 12.1 15 15 15
sum 4.6 4.4 3.4 8.9 8.5 7.7 10 9.6 8.4 15 15 15

Additional Resources and Comments