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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
ERBB3 HER3
Sequence Length (AA) Molecular Weight (Da)
1342 148098
Protein Name
Receptor tyrosine-protein kinase erbB-3
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MRANDALQVL GLLFSLARGS EVGNSQAVCP GTLNGLSVTG DAENQYQTLY
60 70 80 90 100
KLYERCEVVM GNLEIVLTGH NADLSFLQWI REVTGYVLVA MNEFSTLPLP
110 120 130 140 150
NLRVVRGTQV YDGKFAIFVM LNYNTNSSHA LRQLRLTQLT EILSGGVYIE
160 170 180 190 200
KNDKLCHMDT IDWRDIVRDR DAEIVVKDNG RSCPPCHEVC KGRCWGPGSE
210 220 230 240 250
DCQTLTKTIC APQCNGHCFG PNPNQCCHDE CAGGCSGPQD TDCFACRHFN
260 270 280 290 300
DSGACVPRCP QPLVYNKLTF QLEPNPHTKY QYGGVCVASC PHNFVVDQTS
310 320 330 340 350
CVRACPPDKM EVDKNGLKMC EPCGGLCPKA CEGTGSGSRF QTVDSSNIDG
360 370 380 390 400
FVNCTKILGN LDFLITGLNG DPWHKIPALD PEKLNVFRTV REITGYLNIQ
410 420 430 440 450
SWPPHMHNFS VFSNLTTIGG RSLYNRGFSL LIMKNLNVTS LGFRSLKEIS
460 470 480 490 500
AGRIYISANR QLCYHHSLNW TKVLRGPTEE RLDIKHNRPR RDCVAEGKVC
510 520 530 540 550
DPLCSSGGCW GPGPGQCLSC RNYSRGGVCV THCNFLNGEP REFAHEAECF
560 570 580 590 600
SCHPECQPME GTATCNGSGS DTCAQCAHFR DGPHCVSSCP HGVLGAKGPI
610 620 630 640 650
YKYPDVQNEC RPCHENCTQG CKGPELQDCL GQTLVLIGKT HLTMALTVIA
660 670 680 690 700
GLVVIFMMLG GTFLYWRGRR IQNKRAMRRY LERGESIEPL DPSEKANKVL
710 720 730 740 750
ARIFKETELR KLKVLGSGVF GTVHKGVWIP EGESIKIPVC IKVIEDKSGR
760 770 780 790 800
QSFQAVTDHM LAIGSLDHAH IVRLLGLCPG SSLQLVTQYL PLGSLLDHVR
810 820 830 840 850
QHRGALGPQL LLNWGVQIAK GMYYLEEHGM VHRNLAARNV LLKSPSQVQV
860 870 880 890 900
ADFGVADLLP PDDKQLLYSE AKTPIKWMAL ESIHFGKYTH QSDVWSYGVT
910 920 930 940 950
VWELMTFGAE PYAGLRLAEV PDLLEKGERL AQPQICTIDV YMVMVKCWMI
960 970 980 990 1000
DENIRPTFKE LANEFTRMAR DPPRYLVIKR ESGPGIAPGP EPHGLTNKKL
1010 1020 1030 1040 1050
EEVELEPELD LDLDLEAEED NLATTTLGSA LSLPVGTLNR PRGSQSLLSP
1060 1070 1080 1090 1100
SSGYMPMNQG NLGESCQESA VSGSSERCPR PVSLHPMPRG CLASESSEGH
1110 1120 1130 1140 1150
VTGSEAELQE KVSMCRSRSR SRSPRPRGDS AYHSQRHSLL TPVTPLSPPG
1160 1170 1180 1190 1200
LEEEDVNGYV MPDTHLKGTP SSREGTLSSV GLSSVLGTEE EDEDEEYEYM
1210 1220 1230 1240 1250
NRRRRHSPPH PPRPSSLEEL GYEYMDVGSD LSASLGSTQS CPLHPVPIMP
1260 1270 1280 1290 1300
TAGTTPDEDY EYMNRQRDGG GPGGDYAAMG ACPASEQGYE EMRAFQGPGH
1310 1320 1330 1340 1342
QAPHVHYARL KTLRSLEATD SAFDNPDYWH SRLFPKANAQ RT

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-5768 Collapse assay details

Data source: Panorama

Official Gene Symbol
ERBB3
Peptide Sequence
LTFQLEPNPHTK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
268
Peptide End
279
CPTAC ID
CPTAC-5768
Peptide Molecular Mass
1,423.7460
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute
Submitting Lab PI
Amanda Paulovich, John Koomen, Steven A. Carr

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
SCIEX 5500 / Thermo Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Dionex Ultimate 3000
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 18.1 8.1 3.1 26.6 19.1 11.5 32.2 20.7 11.9 15 15 15
y4 (1+) 16.7 8 5.5 22.3 20.7 12.6 27.9 22.2 13.7 15 15 15
sum 17.4 8.1 4 24.5 19.8 11.9 30.1 21.4 12.6 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-5766 Collapse assay details

Data source: Panorama

Official Gene Symbol
ERBB3
Peptide Sequence
SLEATDSAFDNPDYWHSR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
1315
Peptide End
1332
CPTAC ID
CPTAC-5766
Peptide Molecular Mass
2,109.9028
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute
Submitting Lab PI
Amanda Paulovich, John Koomen, Steven A. Carr

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
SCIEX 5500 / Thermo Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Dionex Ultimate 3000
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 30.8 9.5 7.6 35.5 18.5 9.6 47 20.8 12.2 15 15 15
y7 (1+) 18.5 10.1 9.2 32.5 19 11.4 37.4 21.5 14.6 15 15 15
sum 19.9 9.2 8.2 30.6 18.4 9.8 36.5 20.6 12.8 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-5767 Collapse assay details

Data source: Panorama

Official Gene Symbol
ERBB3
Peptide Sequence
YLERGESIEPLDPSEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
680
Peptide End
695
CPTAC ID
CPTAC-5767
Peptide Molecular Mass
1,860.9105
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute
Submitting Lab PI
Amanda Paulovich, John Koomen, Steven A. Carr

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
SCIEX 5500 / Thermo Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Dionex Ultimate 3000
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (2+) 26 8.1 4.7 32.6 16.5 15.6 41.7 18.4 16.3 15 15 15
b9 (1+) 39.1 5.9 5.2 43.1 17.2 14.9 58.2 18.2 15.8 15 15 15
y4 (1+) 24.8 9.2 3.9 32.3 18.7 14.5 40.7 20.8 15 15 15 15
sum 24.2 6.7 3.3 32.3 17 14.5 40.4 18.3 14.9 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5387 Collapse assay details

Data source: Panorama

Official Gene Symbol
ERBB3
Peptide Sequence
ANDALQVLGLLFSLAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
3
Peptide End
18
CPTAC ID
non-CPTAC-5387
Peptide Molecular Mass
1,699.9621
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
RSLCnano
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 44.4 10.5 12.1 36.6 16.5 23.7 57.5 19.6 26.6 15 15 15
y9 (1+) 43.2 9.7 11.1 36.2 16.6 23.1 56.4 19.2 25.6 15 15 15
y10 (1+) 50.9 9.5 12.2 42.9 14 23.7 66.6 16.9 26.7 15 15 15
y11 (1+) 48.1 9.3 10.6 40.7 14.8 24.1 63 17.5 26.3 15 15 15
sum 45.3 9.2 11.4 37.6 15.6 23.5 58.9 18.1 26.1 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5390 Collapse assay details

Data source: Panorama

Official Gene Symbol
ERBB3
Peptide Sequence
GDSAYHSQR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1128
Peptide End
1136
CPTAC ID
non-CPTAC-5390
Peptide Molecular Mass
1,019.4421
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
RSLCnano
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 11.4 4.3 3.8 10.7 4 4.2 15.6 5.9 5.7 15 15 15
y4 (1+) 7.6 4.6 4.7 10.5 5.7 4.5 13 7.3 6.5 15 15 15
y5 (1+) 13.2 5.2 3.1 13.1 5 5 18.6 7.2 5.9 15 15 15
y6 (1+) 31.8 9.3 4.1 27.9 12.8 6.2 42.3 15.8 7.4 15 15 15
sum 4.7 2.6 3 6.6 3.3 3.6 8.1 4.2 4.7 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5548 Collapse assay details

Data source: Panorama

Official Gene Symbol
ERBB3
Peptide Sequence
LTFQLEPNPHTK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
6
Peptide Start
268
Peptide End
279
CPTAC ID
non-CPTAC-5548
Peptide Molecular Mass
1,423.7460
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
Cell Line
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
SIS Peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
RSLCnano
Column Packing
C18 PepMap100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 17.8 6.3 3.2 24.4 20.3 11.6 30.2 21.3 12 14 14 14
y5 (1+) 29 12.5 4.9 35.7 19.7 11.9 46 23.3 12.9 14 14 14
y6 (1+) 19 5.6 2.4 28.9 19.6 11.7 34.6 20.4 11.9 14 14 14
y8 (1+) 19 6.3 3.2 30.2 18.6 11.6 35.7 19.6 12 14 14 14
sum 18.2 5.7 2.4 27.1 19.6 11.5 32.6 20.4 11.7 14 14 14


Additional Resources and Comments


Assay Details for non-CPTAC-5550 Collapse assay details

Data source: Panorama

Official Gene Symbol
ERBB3
Peptide Sequence
SLEATDSAFDNPDYWHSR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
6
Peptide Start
1315
Peptide End
1332
CPTAC ID
non-CPTAC-5550
Peptide Molecular Mass
2,109.9028
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
Cell Line
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
SIS Peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
RSLCnano
Column Packing
C18 PepMap100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y14 (2+) 26.2 11.5 7.1 23.5 15.1 9.6 35.2 19 11.9 11 11 11
y15 (2+) 24.5 8.2 7.4 29.1 20.5 10.6 38 22.1 12.9 11 11 11
y16 (2+) 22.2 7.3 6.6 28.2 18.4 8.8 35.9 19.8 11 11 11 11
y5 (1+) 27 9.7 4.6 34.9 20.6 6.8 44.1 22.8 8.2 11 11 11
y7 (1+) 17.4 9.5 6.4 31.4 21.4 7.9 35.9 23.4 10.2 11 11 11
sum 21.5 8 6.5 26.4 18.5 8.6 34 20.2 10.8 11 11 11


Additional Resources and Comments


Assay Details for non-CPTAC-5549 Collapse assay details

Data source: Panorama

Official Gene Symbol
ERBB3
Peptide Sequence
YLERGESIEPLDPSEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
6
Peptide Start
680
Peptide End
695
CPTAC ID
non-CPTAC-5549
Peptide Molecular Mass
1,860.9105
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
Cell Line
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
SIS Peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
RSLCnano
Column Packing
C18 PepMap100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b9 (1+) 29.1 5.9 3 36.4 18 14.9 46.6 18.9 15.2 14 14 14
y7 (2+) 24.1 7.6 2.7 31.7 15.1 15.1 39.8 16.9 15.3 14 14 14
y4 (1+) 26.8 8 3.3 34.8 17.9 15.1 43.9 19.6 15.5 14 14 14
y5 (1+) 25.9 11.3 4.5 36.5 14.9 17.1 44.8 18.7 17.7 14 14 14
y7 (1+) 23 6.6 2.3 31 17.1 15.7 38.6 18.3 15.9 14 14 14
sum 23.5 6 2 32 16 15.2 39.7 17.1 15.3 14 14 14


Additional Resources and Comments


Assay Details for CPTAC-3177 Collapse assay details

Data source: Panorama

Official Gene Symbol
ERBB3
Peptide Sequence
LTFQLEPNPHTK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
268
Peptide End
279
CPTAC ID
CPTAC-3177
Peptide Molecular Mass
1,423.7460
Species
Homo Sapiens
Assay Type
Direct MRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 4.8 3.3 3.8 5 5 4.5 6.9 6 5.9 15 15 15
y8 (1+) 7.4 3.6 3.3 8.6 6.1 4.9 11.3 7.1 5.9 15 15 15
y6 (2+) 9.1 4 4.1 10 6 5.5 13.5 7.2 6.9 15 15 15
sum 5.6 3.1 3.5 5.7 5.2 4.5 8 6.1 5.7 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-3176 Collapse assay details

Data source: Panorama

Official Gene Symbol
ERBB3
Peptide Sequence
VLGSGVFGTVHK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
714
Peptide End
725
CPTAC ID
CPTAC-3176
Peptide Molecular Mass
1,199.6663
Species
Homo Sapiens
Assay Type
Direct MRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 5.8 3.7 5.7 7.4 4 5.6 9.4 5.4 8 15 15 15
y8 (1+) 5.9 4 6.3 11.2 4.9 5.7 12.7 6.3 8.5 15 15 15
y5 (1+) 4.7 3.6 4.3 6.6 4.2 4.7 8.1 5.5 6.4 15 15 15
sum 4.8 3.5 5.1 6.8 3.8 5.1 8.3 5.2 7.2 15 15 15


Additional Resources and Comments