ERBB3, CPTAC-5548 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
ERBB3	HER3

Sequence Length (AA)	Molecular Weight (Da)
1342	148098

Protein Name
Receptor tyrosine-protein kinase erbB-3

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MRANDALQVL	GLLFSLARGS	EVGNSQAVCP	GTLNGLSVTG	DAENQYQTLY
60	70	80	90	100
KLYERCEVVM	GNLEIVLTGH	NADLSFLQWI	REVTGYVLVA	MNEFSTLPLP
110	120	130	140	150
NLRVVRGTQV	YDGKFAIFVM	LNYNTNSSHA	LRQLRLTQLT	EILSGGVYIE
160	170	180	190	200
KNDKLCHMDT	IDWRDIVRDR	DAEIVVKDNG	RSCPPCHEVC	KGRCWGPGSE
210	220	230	240	250
DCQTLTKTIC	APQCNGHCFG	PNPNQCCHDE	CAGGCSGPQD	TDCFACRHFN
260	270	280	290	300
DSGACVPRCP	QPLVYNKLTF	QLEPNPHTKY	QYGGVCVASC	PHNFVVDQTS
310	320	330	340	350
CVRACPPDKM	EVDKNGLKMC	EPCGGLCPKA	CEGTGSGSRF	QTVDSSNIDG
360	370	380	390	400
FVNCTKILGN	LDFLITGLNG	DPWHKIPALD	PEKLNVFRTV	REITGYLNIQ
410	420	430	440	450
SWPPHMHNFS	VFSNLTTIGG	RSLYNRGFSL	LIMKNLNVTS	LGFRSLKEIS
460	470	480	490	500
AGRIYISANR	QLCYHHSLNW	TKVLRGPTEE	RLDIKHNRPR	RDCVAEGKVC
510	520	530	540	550
DPLCSSGGCW	GPGPGQCLSC	RNYSRGGVCV	THCNFLNGEP	REFAHEAECF
560	570	580	590	600
SCHPECQPME	GTATCNGSGS	DTCAQCAHFR	DGPHCVSSCP	HGVLGAKGPI
610	620	630	640	650
YKYPDVQNEC	RPCHENCTQG	CKGPELQDCL	GQTLVLIGKT	HLTMALTVIA
660	670	680	690	700
GLVVIFMMLG	GTFLYWRGRR	IQNKRAMRRY	LERGESIEPL	DPSEKANKVL
710	720	730	740	750
ARIFKETELR	KLKVLGSGVF	GTVHKGVWIP	EGESIKIPVC	IKVIEDKSGR
760	770	780	790	800
QSFQAVTDHM	LAIGSLDHAH	IVRLLGLCPG	SSLQLVTQYL	PLGSLLDHVR
810	820	830	840	850
QHRGALGPQL	LLNWGVQIAK	GMYYLEEHGM	VHRNLAARNV	LLKSPSQVQV
860	870	880	890	900
ADFGVADLLP	PDDKQLLYSE	AKTPIKWMAL	ESIHFGKYTH	QSDVWSYGVT
910	920	930	940	950
VWELMTFGAE	PYAGLRLAEV	PDLLEKGERL	AQPQICTIDV	YMVMVKCWMI
960	970	980	990	1000
DENIRPTFKE	LANEFTRMAR	DPPRYLVIKR	ESGPGIAPGP	EPHGLTNKKL
1010	1020	1030	1040	1050
EEVELEPELD	LDLDLEAEED	NLATTTLGSA	LSLPVGTLNR	PRGSQSLLSP
1060	1070	1080	1090	1100
SSGYMPMNQG	NLGESCQESA	VSGSSERCPR	PVSLHPMPRG	CLASESSEGH
1110	1120	1130	1140	1150
VTGSEAELQE	KVSMCRSRSR	SRSPRPRGDS	AYHSQRHSLL	TPVTPLSPPG
1160	1170	1180	1190	1200
LEEEDVNGYV	MPDTHLKGTP	SSREGTLSSV	GLSSVLGTEE	EDEDEEYEYM
1210	1220	1230	1240	1250
NRRRRHSPPH	PPRPSSLEEL	GYEYMDVGSD	LSASLGSTQS	CPLHPVPIMP
1260	1270	1280	1290	1300
TAGTTPDEDY	EYMNRQRDGG	GPGGDYAAMG	ACPASEQGYE	EMRAFQGPGH
1310	1320	1330	1340	1342
QAPHVHYARL	KTLRSLEATD	SAFDNPDYWH	SRLFPKANAQ	RT

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-5768 Collapse assay details

Data source: Panorama

Official Gene Symbol: ERBB3
Peptide Sequence: LTFQLEPNPHTK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 3
Peptide Start: 268
Peptide End: 279

CPTAC ID: CPTAC-5768
Peptide Molecular Mass: 1,423.7460
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: Immuno-MRM
Matrix: Cell Lysate
Submitting Laboratory: Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute
Submitting Lab PI: Amanda Paulovich, John Koomen, Steven A. Carr

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: SCIEX 5500 / Thermo Quantiva
Internal Standard: Stable Isotope Labeled Standard
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Dionex Ultimate 3000
Column Packing: C18 PepMap 100
Column Dimensions: 75 micron ID x 25 cm length
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	18.1	8.1	3.1	26.6	19.1	11.5	32.2	20.7	11.9	15	15	15
y4 (1+)	16.7	8	5.5	22.3	20.7	12.6	27.9	22.2	13.7	15	15	15
sum	17.4	8.1	4	24.5	19.8	11.9	30.1	21.4	12.6	15	15	15

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (2+)	26	8.1	4.7	32.6	16.5	15.6	41.7	18.4	16.3	15	15	15
b9 (1+)	39.1	5.9	5.2	43.1	17.2	14.9	58.2	18.2	15.8	15	15	15
y4 (1+)	24.8	9.2	3.9	32.3	18.7	14.5	40.7	20.8	15	15	15	15
sum	24.2	6.7	3.3	32.3	17	14.5	40.4	18.3	14.9	15	15	15

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8 (1+)	44.4	10.5	12.1	36.6	16.5	23.7	57.5	19.6	26.6	15	15	15
y9 (1+)	43.2	9.7	11.1	36.2	16.6	23.1	56.4	19.2	25.6	15	15	15
y10 (1+)	50.9	9.5	12.2	42.9	14	23.7	66.6	16.9	26.7	15	15	15
y11 (1+)	48.1	9.3	10.6	40.7	14.8	24.1	63	17.5	26.3	15	15	15
sum	45.3	9.2	11.4	37.6	15.6	23.5	58.9	18.1	26.1	15	15	15

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y3 (1+)	11.4	4.3	3.8	10.7	4	4.2	15.6	5.9	5.7	15	15	15
y4 (1+)	7.6	4.6	4.7	10.5	5.7	4.5	13	7.3	6.5	15	15	15
y5 (1+)	13.2	5.2	3.1	13.1	5	5	18.6	7.2	5.9	15	15	15
y6 (1+)	31.8	9.3	4.1	27.9	12.8	6.2	42.3	15.8	7.4	15	15	15
sum	4.7	2.6	3	6.6	3.3	3.6	8.1	4.2	4.7	15	15	15

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y4 (1+)	17.8	6.3	3.2	24.4	20.3	11.6	30.2	21.3	12	14	14	14
y5 (1+)	29	12.5	4.9	35.7	19.7	11.9	46	23.3	12.9	14	14	14
y6 (1+)	19	5.6	2.4	28.9	19.6	11.7	34.6	20.4	11.9	14	14	14
y8 (1+)	19	6.3	3.2	30.2	18.6	11.6	35.7	19.6	12	14	14	14
sum	18.2	5.7	2.4	27.1	19.6	11.5	32.6	20.4	11.7	14	14	14

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ERBB3

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-5768 Collapse assay details

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Assay Parameters Collapse assay parameters

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Assay Parameters Collapse assay parameters

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Assay Parameters Collapse assay parameters

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