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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

BTLA

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
BTLA N/A
Sequence Length (AA) Molecular Weight (Da)
289 32834
Protein Name
B- and T-lymphocyte attenuator
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MKTLPAMLGT GKLFWVFFLI PYLDIWNIHG KESCDVQLYI KRQSEHSILA
60 70 80 90 100
GDPFELECPV KYCANRPHVT WCKLNGTTCV KLEDRQTSWK EEKNISFFIL
110 120 130 140 150
HFEPVLPNDN GSYRCSANFQ SNLIESHSTT LYVTDVKSAS ERPSKDEMAS
160 170 180 190 200
RPWLLYRLLP LGGLPLLITT CFCLFCCLRR HQGKQNELSD TAGREINLVD
210 220 230 240 250
AHLKSEQTEA STRQNSQVLL SETGIYDNDP DLCFRMQEGS EVYSNPCLEE
260 270 280 289
NKPGIVYASL NHSVIGPNSR LARNVKEAPT EYASICVRS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-6043 Collapse assay details

Data source: Panorama

Official Gene Symbol
BTLA
Peptide Modified Sequence
EAPTEYASIC[+57.021464]VR
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
286
Peptide - Site of Modification
10
Peptide Start
277
Peptide End
288
CPTAC ID
CPTAC-6043
Peptide Molecular Mass
1,394.6500
Species
Human
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
FFPE lung tumor tissue lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Whiteaker JR, Zhao L, Schoenherr RM, Huang D, Lundeen RA, Voytovich U, Kennedy JJ, Ivey RG, Lin C, Murillo OD, Lorentzen TD, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Reading JJ, Perry CD, Richardson CW, Garcia-Buntley SS, Bocik W, Hewitt SM, Chowdhury S, Vandermeer J, Smith SD, Gopal AK, Ramchurren N, Fling SP, Wang P, Paulovich AG. A multiplexed assay for quantifying immunomodulatory proteins supports correlative studies in immunotherapy clinical trials. Front Oncol. 2023 May 2;13:1168710. doi: 10.3389/fonc.2023.1168710. PMID: 37205196; PMCID: PMC10185886.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTRAP 5500
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Eksigent nanoLC 425
Column Packing
3 µm ChromXP C18EP, 120 Å
Column Dimensions
150 x 0.075 mm
Flow Rate
0.3 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b7 (1+) 40.4 42.1 42 52 48.8 54.8 65.8 64.5 69 15 15 15
y3 (1+) 23.5 15.6 23.8 24.5 23.1 27.3 33.9 27.9 36.2 15 15 15
y4 (1+) 25 21.6 19 36 25.6 15.9 43.8 33.5 24.8 15 15 15
y5 (1+) 17.8 19.2 15.6 29.9 19 19.5 34.8 27 25 15 15 15
y6 (1+) 23.9 9.9 7.2 25.9 16.9 10.4 35.2 19.6 12.6 15 15 15
y7 (1+) 22.8 14.9 9.2 25.3 21 6.8 34.1 25.7 11.4 15 15 15
sum 16.5 12.5 9 21.6 17.4 10.5 27.2 21.4 13.8 15 15 15

Additional Resources and Comments

Resources
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View Peptide in Panorama
View Protein in Panorama
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