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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

MGMT

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
MGMT N/A
Sequence Length (AA) Molecular Weight (Da)
207 21646
Protein Name
Methylated-DNA--protein-cysteine methyltransferase
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MDKDCEMKRT TLDSPLGKLE LSGCEQGLHE IKLLGKGTSA ADAVEVPAPA
60 70 80 90 100
AVLGGPEPLM QCTAWLNAYF HQPEAIEEFP VPALHHPVFQ QESFTRQVLW
110 120 130 140 150
KLLKVVKFGE VISYQQLAAL AGNPKAARAV GGAMRGNPVP ILIPCHRVVC
160 170 180 190 200
SSGAVGNYSG GLAVKEWLLA HEGHRLGKPG LGGSSGLAGA WLKGAGATSG
207
SPPAGRN

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-6068 Collapse assay details

Data source: Panorama

Official Gene Symbol
MGMT
Peptide Modified Sequence
GNPVPILIPC[+57.021464]HR
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
145
Peptide - Site of Modification
10
Peptide Start
136
Peptide End
147
CPTAC ID
CPTAC-6068
Peptide Molecular Mass
1,371.7445
Species
Human
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
FFPE lung tumor tissue lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Whiteaker JR, Zhao L, Schoenherr RM, Huang D, Lundeen RA, Voytovich U, Kennedy JJ, Ivey RG, Lin C, Murillo OD, Lorentzen TD, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Reading JJ, Perry CD, Richardson CW, Garcia-Buntley SS, Bocik W, Hewitt SM, Chowdhury S, Vandermeer J, Smith SD, Gopal AK, Ramchurren N, Fling SP, Wang P, Paulovich AG. A multiplexed assay for quantifying immunomodulatory proteins supports correlative studies in immunotherapy clinical trials. Front Oncol. 2023 May 2;13:1168710. doi: 10.3389/fonc.2023.1168710. PMID: 37205196; PMCID: PMC10185886.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTRAP 5500
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Eksigent nanoLC 425
Column Packing
3 µm ChromXP C18EP, 120 Å
Column Dimensions
150 x 0.075 mm
Flow Rate
0.3 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b4 (1+) 31.6 14.3 26.6 32.2 14 23.5 45.1 20 35.5 15 15 15
b7 (1+) 19.4 12.5 13.5 24.5 13.3 14.7 31.3 18.3 20 15 15 15
y4 (1+) 12.3 8.3 7.3 20.4 5.7 8.1 23.8 10.1 10.9 15 15 15
y5 (1+) 18.9 9.5 11 38.8 9.5 16.6 43.2 13.4 19.9 15 15 15
y6 (1+) 21.1 10 9.9 22.4 10.1 9.9 30.8 14.2 14 15 15 15
y9 (2+) 20.2 11.9 12.5 17.1 11.1 14.8 26.5 16.3 19.4 15 15 15
sum 12.5 7.3 7.3 18.1 5.8 9.3 22 9.3 11.8 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments