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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

TAP1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
TAP1 ABCB2, PSF1, RING4, Y3
Sequence Length (AA) Molecular Weight (Da)
748 80965
Protein Name
Antigen peptide transporter 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MASSRCPAPR GCRCLPGASL AWLGTVLLLL ADWVLLRTAL PRIFSLLVPT
60 70 80 90 100
ALPLLRVWAV GLSRWAVLWL GACGVLRATV GSKSENAGAQ GWLAALKPLA
110 120 130 140 150
AALGLALPGL ALFRELISWG APGSADSTRL LHWGSHPTAF VVSYAAALPA
160 170 180 190 200
AALWHKLGSL WVPGGQGGSG NPVRRLLGCL GSETRRLSLF LVLVVLSSLG
210 220 230 240 250
EMAIPFFTGR LTDWILQDGS ADTFTRNLTL MSILTIASAV LEFVGDGIYN
260 270 280 290 300
NTMGHVHSHL QGEVFGAVLR QETEFFQQNQ TGNIMSRVTE DTSTLSDSLS
310 320 330 340 350
ENLSLFLWYL VRGLCLLGIM LWGSVSLTMV TLITLPLLFL LPKKVGKWYQ
360 370 380 390 400
LLEVQVRESL AKSSQVAIEA LSAMPTVRSF ANEEGEAQKF REKLQEIKTL
410 420 430 440 450
NQKEAVAYAV NSWTTSISGM LLKVGILYIG GQLVTSGAVS SGNLVTFVLY
460 470 480 490 500
QMQFTQAVEV LLSIYPRVQK AVGSSEKIFE YLDRTPRCPP SGLLTPLHLE
510 520 530 540 550
GLVQFQDVSF AYPNRPDVLV LQGLTFTLRP GEVTALVGPN GSGKSTVAAL
560 570 580 590 600
LQNLYQPTGG QLLLDGKPLP QYEHRYLHRQ VAAVGQEPQV FGRSLQENIA
610 620 630 640 650
YGLTQKPTME EITAAAVKSG AHSFISGLPQ GYDTEVDEAG SQLSGGQRQA
660 670 680 690 700
VALARALIRK PCVLILDDAT SALDANSQLQ VEQLLYESPE RYSRSVLLIT
710 720 730 740 748
QHLSLVEQAD HILFLEGGAI REGGTHQQLM EKKGCYWAMV QAPADAPE

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-6069 Collapse assay details

Data source: Panorama

Official Gene Symbol
TAP1
Peptide Sequence
ELISWGAPGSADSTR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
115
Peptide End
129
CPTAC ID
CPTAC-6069
Peptide Molecular Mass
1,545.7423
Species
Human
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
FFPE lung tumor tissue lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Whiteaker JR, Zhao L, Schoenherr RM, Huang D, Lundeen RA, Voytovich U, Kennedy JJ, Ivey RG, Lin C, Murillo OD, Lorentzen TD, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Reading JJ, Perry CD, Richardson CW, Garcia-Buntley SS, Bocik W, Hewitt SM, Chowdhury S, Vandermeer J, Smith SD, Gopal AK, Ramchurren N, Fling SP, Wang P, Paulovich AG. A multiplexed assay for quantifying immunomodulatory proteins supports correlative studies in immunotherapy clinical trials. Front Oncol. 2023 May 2;13:1168710. doi: 10.3389/fonc.2023.1168710. PMID: 37205196; PMCID: PMC10185886.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTRAP 5500
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Eksigent nanoLC 425
Column Packing
3 µm ChromXP C18EP, 120 Å
Column Dimensions
150 x 0.075 mm
Flow Rate
0.3 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 18.4 11.3 25.6 43.6 12.1 18.1 47.3 16.6 31.4 15 15 15
y11 (1+) 16.9 16.8 27.2 48.9 18.2 25.9 51.7 24.8 37.6 15 15 15
y12 (1+) 25 8.9 24.3 42.8 11.6 28.1 49.6 14.6 37.1 15 15 15
y8 (1+) 25.8 11.5 26.1 46.3 12.2 20.2 53 16.8 33 15 15 15
y9 (1+) 23.8 15.9 29.6 41 14.3 18.5 47.4 21.4 34.9 15 15 15
y12 (2+) 37.6 18.1 30 73 24.8 34.7 82.1 30.7 45.9 15 15 15
sum 19.8 10 25.7 42.8 11.8 18.5 47.2 15.5 31.7 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
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