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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

JAK1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
JAK1 JAK1A, JAK1B
Sequence Length (AA) Molecular Weight (Da)
1154 133277
Protein Name
Tyrosine-protein kinase JAK1
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MQYLNIKEDC NAMAFCAKMR SSKKTEVNLE APEPGVEVIF YLSDREPLRL
60 70 80 90 100
GSGEYTAEEL CIRAAQACRI SPLCHNLFAL YDENTKLWYA PNRTITVDDK
110 120 130 140 150
MSLRLHYRMR FYFTNWHGTN DNEQSVWRHS PKKQKNGYEK KKIPDATPLL
160 170 180 190 200
DASSLEYLFA QGQYDLVKCL APIRDPKTEQ DGHDIENECL GMAVLAISHY
210 220 230 240 250
AMMKKMQLPE LPKDISYKRY IPETLNKSIR QRNLLTRMRI NNVFKDFLKE
260 270 280 290 300
FNNKTICDSS VSTHDLKVKY LATLETLTKH YGAEIFETSM LLISSENEMN
310 320 330 340 350
WFHSNDGGNV LYYEVMVTGN LGIQWRHKPN VVSVEKEKNK LKRKKLENKH
360 370 380 390 400
KKDEEKNKIR EEWNNFSYFP EITHIVIKES VVSINKQDNK KMELKLSSHE
410 420 430 440 450
EALSFVSLVD GYFRLTADAH HYLCTDVAPP LIVHNIQNGC HGPICTEYAI
460 470 480 490 500
NKLRQEGSEE GMYVLRWSCT DFDNILMTVT CFEKSEQVQG AQKQFKNFQI
510 520 530 540 550
EVQKGRYSLH GSDRSFPSLG DLMSHLKKQI LRTDNISFML KRCCQPKPRE
560 570 580 590 600
ISNLLVATKK AQEWQPVYPM SQLSFDRILK KDLVQGEHLG RGTRTHIYSG
610 620 630 640 650
TLMDYKDDEG TSEEKKIKVI LKVLDPSHRD ISLAFFEAAS MMRQVSHKHI
660 670 680 690 700
VYLYGVCVRD VENIMVEEFV EGGPLDLFMH RKSDVLTTPW KFKVAKQLAS
710 720 730 740 750
ALSYLEDKDL VHGNVCTKNL LLAREGIDSE CGPFIKLSDP GIPITVLSRQ
760 770 780 790 800
ECIERIPWIA PECVEDSKNL SVAADKWSFG TTLWEICYNG EIPLKDKTLI
810 820 830 840 850
EKERFYESRC RPVTPSCKEL ADLMTRCMNY DPNQRPFFRA IMRDINKLEE
860 870 880 890 900
QNPDIVSEKK PATEVDPTHF EKRFLKRIRD LGEGHFGKVE LCRYDPEGDN
910 920 930 940 950
TGEQVAVKSL KPESGGNHIA DLKKEIEILR NLYHENIVKY KGICTEDGGN
960 970 980 990 1000
GIKLIMEFLP SGSLKEYLPK NKNKINLKQQ LKYAVQICKG MDYLGSRQYV
1010 1020 1030 1040 1050
HRDLAARNVL VESEHQVKIG DFGLTKAIET DKEYYTVKDD RDSPVFWYAP
1060 1070 1080 1090 1100
ECLMQSKFYI ASDVWSFGVT LHELLTYCDS DSSPMALFLK MIGPTHGQMT
1110 1120 1130 1140 1150
VTRLVNTLKE GKRLPCPPNC PDEVYQLMRK CWEFQPSNRT SFQNLIEGFE
1154
ALLK

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-6216 Collapse assay details

Data source: Panorama

Official Gene Symbol
JAK1
Peptide Sequence
LSDPGIPITVLSR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
7
Peptide Start
737
Peptide End
749
CPTAC ID
CPTAC-6216
Peptide Molecular Mass
1,366.7820
Species
Homo Sapiens
Assay Type
Enrichment MRM
Enrichment Method
Peptide Immunoaffinity
Matrix
Frozen tissue
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTRAP 5500
Internal Standard
Extended heavy peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Eksigent
Column Packing
Reprosil C18, 3um
Column Dimensions
75µm x 15cm
Flow Rate
0.3 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 10.8 6.9 5.4 11.5 10.3 13.8 15.8 12.4 14.8 15 15 15
y10 (1+) 8.8 5.1 7.7 14.8 5 9 17.2 7.1 11.8 15 15 15
b5 (1+) 10.3 7 4 15.7 6.1 6.3 18.8 9.3 7.5 15 15 15
b6 (1+) 13.3 6 4.4 14.9 6.3 7.7 20 8.7 8.9 15 15 15
sum 9.9 5.5 2.7 12.1 7.4 9.1 15.6 9.2 9.5 15 15 15

Additional Resources and Comments

Resources
Download SOPs
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View in CPTC Antibody Portal
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Assay Details for CPTAC-1251 Collapse assay details

Data source: Panorama

Official Gene Symbol
JAK1
Peptide Sequence
QLASALSYLEDK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
697
Peptide End
708
CPTAC ID
CPTAC-1251
Peptide Molecular Mass
1,336.6874
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 11.8 3.7 8.9 23.6 7.2 17 26.4 8.1 19.2 15 15 15
y6 (1+) 7.6 6.2 3.9 14.4 10.3 13.1 16.3 12 13.7 15 15 15
y7 (1+) 7 7.1 4.3 14.3 11.8 15.4 15.9 13.8 16 15 15 15
sum 3.3 5.6 4 13.6 10 14.3 14 11.5 14.8 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-3186 Collapse assay details

Data source: Panorama

Official Gene Symbol
JAK1
Peptide Modified Sequence
C[+57.0]WEFQPSNR
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
1132
Peptide - Site of Modification
1
Peptide Start
1131
Peptide End
1139
CPTAC ID
CPTAC-3186
Peptide Molecular Mass
1,222.5189
Species
Homo Sapiens
Assay Type
Direct MRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 4.1 4.8 2.9 6.1 4.6 3 7.3 6.6 4.2 15 15 15
y7 (1+) 12.6 6.4 2.7 21.8 6.6 2.7 25.2 9.2 3.8 15 15 15
y6 (1+) 6.7 4 3.6 8.1 4.9 3.8 10.5 6.3 5.2 15 15 15
sum 6.2 5.1 2.6 9.6 5.3 2.9 11.4 7.4 3.9 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-3185 Collapse assay details

Data source: Panorama

Official Gene Symbol
JAK1
Peptide Sequence
KPATEVDPTHFEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
860
Peptide End
872
CPTAC ID
CPTAC-3185
Peptide Molecular Mass
1,497.7464
Species
Homo Sapiens
Assay Type
Direct MRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 22.6 5.5 6.5 27.3 5.6 7.8 35.4 7.8 10.2 15 15 15
y5 (1+) 16.5 4.8 6.7 18.1 9.6 8.3 24.5 10.7 10.7 15 15 15
y6 (2+) 5.3 4.2 5 7.9 5.3 6.3 9.5 6.8 8 15 15 15
sum 7.9 4 4.5 9.3 5.7 6.1 12.2 7 7.6 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments