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Overview Data source: UniProt

Official Gene Symbol Other Aliases
AKT1S1 PRAS40
Sequence Length (AA) Molecular Weight (Da)
256 27383
Protein Name
Proline-rich AKT1 substrate 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MASGRPEELW EAVVGAAERF RARTGTELVL LTAAPPPPPR PGPCAYAAHG
60 70 80 90 100
RGALAEAARR CLHDIALAHR AATAARPPAP PPAPQPPSPT PSPPRPTLAR
110 120 130 140 150
EDNEEDEDEP TETETSGEQL GISDNGGLFV MDEDATLQDL PPFCESDPES
160 170 180 190 200
TDDGSLSEET PAGPPTCSVP PASALPTQQY AKSLPVSVPV WGFKEKRTEA
210 220 230 240 250
RSSDEENGPP SSPDLDRIAA SMRALVLREA EDTQVFGDLP RPRLNTSDFQ
256
KLKRKY

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-785 Collapse assay details

Data source: Panorama

Official Gene Symbol
AKT1S1
Peptide Modified Sequence
C[+57.0]LHDIALAHR
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
61
Peptide - Site of Modification
1
Peptide Start
61
Peptide End
70
CPTAC ID
CPTAC-785
Peptide Molecular Mass
1,204.6135
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 17.8 9.8 7.2 24.4 9.7 12.3 30.2 13.8 14.3 15 15 15
y4 (1+) 30.7 15.5 7.5 27.9 11 13.9 41.5 19 15.8 15 15 15
b6 (1+) 32 23.5 12.9 33.3 27.1 14.6 46.2 35.9 19.5 15 15 15
b5 (1+) 48.8 16.3 16.1 43.9 18.6 16.7 65.6 24.7 23.2 15 15 15
b4 (1+) 19.3 10.3 9 17.7 10.9 10.1 26.2 15 13.5 15 15 15
b3 (1+) 29 17.5 18.4 27.5 21.6 18.2 40 27.8 25.9 15 15 15
sum 8.5 6.2 3.8 9.3 6 6.7 12.6 8.6 7.7 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-1038 Collapse assay details

Data source: Panorama

Official Gene Symbol
AKT1S1
Peptide Modified Sequence
LNT[+80.0]SDFQK
Modification Type
Phospho (ST)
Protein - Site of Modification
246
Peptide - Site of Modification
3
Peptide Start
244
Peptide End
251
CPTAC ID
CPTAC-1038
Peptide Molecular Mass
1,031.4325
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 21.7 15.1 10.6 28 15.1 11.2 35.4 21.4 15.4 15 15 15
y3 (1+) 12.9 5.6 8.4 15 7.1 6.6 19.8 9 10.7 15 15 15
y5 (1+) 30.8 11.1 8.4 25.9 11.3 10.8 40.2 15.8 13.7 15 15 15
sum 14.8 6.1 4.2 15.6 6.4 5.1 21.5 8.8 6.6 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-787 Collapse assay details

Data source: Panorama

Official Gene Symbol
AKT1S1
Peptide Sequence
SLPVSVPVWGFK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
183
Peptide End
194
CPTAC ID
CPTAC-787
Peptide Molecular Mass
1,314.7336
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 9.7 6.7 5 7.3 7 5.4 12.1 9.7 7.4 15 15 15
y4 (1+) 6.8 4.5 1.7 10.1 5.5 2.5 12.2 7.1 3 15 15 15
y6 (1+) 4.2 2.3 1.8 4.8 2.9 2.8 6.4 3.7 3.3 15 15 15
y7 (1+) 9.6 6.8 8.4 12.4 8.7 9 15.7 11 12.3 15 15 15
y8 (1+) 5.1 3.9 2.7 7.3 4.3 3.1 8.9 5.8 4.1 15 15 15
y9 (1+) 10.9 4.3 4.3 11.1 4.5 5.2 15.6 6.2 6.7 15 15 15
sum 1.8 1.3 1.1 2.4 2.1 1.9 3 2.5 2.2 15 15 15

Additional Resources and Comments