AKT1S1, CPTAC-5698 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
AKT1S1	PRAS40

Sequence Length (AA)	Molecular Weight (Da)
256	27383

Protein Name
Proline-rich AKT1 substrate 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MASGRPEELW	EAVVGAAERF	RARTGTELVL	LTAAPPPPPR	PGPCAYAAHG
60	70	80	90	100
RGALAEAARR	CLHDIALAHR	AATAARPPAP	PPAPQPPSPT	PSPPRPTLAR
110	120	130	140	150
EDNEEDEDEP	TETETSGEQL	GISDNGGLFV	MDEDATLQDL	PPFCESDPES
160	170	180	190	200
TDDGSLSEET	PAGPPTCSVP	PASALPTQQY	AKSLPVSVPV	WGFKEKRTEA
210	220	230	240	250
RSSDEENGPP	SSPDLDRIAA	SMRALVLREA	EDTQVFGDLP	RPRLNTSDFQ
256
KLKRKY

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-6250 Collapse assay details

Data source: Panorama

Official Gene Symbol: AKT1S1
Peptide Modified Sequence: LNT[+79.966331]SDFQK
Modification Type: Phospho (ST)
Protein - Site of Modification: N/A
Peptide - Site of Modification: 8
Peptide Start: 244
Peptide End: 251

CPTAC ID: non-CPTAC-6250
Peptide Molecular Mass: 1,031.4325
Species: Human
Assay Type: Enrichment MRM
Enrichment Method: IMAC
Matrix: SKBR3 cells
Submitting Laboratory: Cell Signaling Technology
Submitting Lab PI: Yiying Zhu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Orbitrap Q Exactive (Thermo)
Internal Standard: Heavy isotope-labeled peptides
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N F

LC: EASY-nLC II (Thermo)
Column Packing: self-packed
Column Dimensions: ID 100um, length 15cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y5 (1+)	6.3	5.3	4.7	6	4.9	6	8.7	7.2	7.6	15	15	15
y7 (1+)	8.8	5.3	4	8.8	5.5	6.8	12.4	7.6	7.9	15	15	15
y7-98 (1+)	6.4	5	4.7	6	6	6.7	8.8	7.8	8.2	15	15	15
y6 (1+)	10.5	6.3	6.1	8.4	8.3	6	13.4	10.4	8.6	15	15	15
y6-98 (1+)	7	4.1	4	6.7	4.4	5.8	9.7	6	7	15	15	15
y7-98 (2+)	5.2	4.8	4.6	4.8	5.3	5.7	7.1	7.2	7.3	15	15	15
sum	6	4.5	4.3	5.4	4.9	5.8	8.1	6.7	7.2	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-5333 Collapse assay details

Data source: Panorama

Official Gene Symbol: AKT1S1
Peptide Sequence: LNTSDFQK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 244
Peptide End: 251

CPTAC ID: non-CPTAC-5333
Peptide Molecular Mass: 951.4662
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Cell Lysate
Submitting Laboratory: Moffitt Cancer Center
Submitting Lab PI: John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.