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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
AKT1S1 PRAS40
Sequence Length (AA) Molecular Weight (Da)
256 27383
Protein Name
Proline-rich AKT1 substrate 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MASGRPEELW EAVVGAAERF RARTGTELVL LTAAPPPPPR PGPCAYAAHG
60 70 80 90 100
RGALAEAARR CLHDIALAHR AATAARPPAP PPAPQPPSPT PSPPRPTLAR
110 120 130 140 150
EDNEEDEDEP TETETSGEQL GISDNGGLFV MDEDATLQDL PPFCESDPES
160 170 180 190 200
TDDGSLSEET PAGPPTCSVP PASALPTQQY AKSLPVSVPV WGFKEKRTEA
210 220 230 240 250
RSSDEENGPP SSPDLDRIAA SMRALVLREA EDTQVFGDLP RPRLNTSDFQ
256
KLKRKY

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-6250 Collapse assay details

Data source: Panorama

Official Gene Symbol
AKT1S1
Peptide Modified Sequence
LNT[+79.966331]SDFQK
Modification Type
Phospho (ST)
Protein - Site of Modification
N/A
Peptide - Site of Modification
8
Peptide Start
244
Peptide End
251
CPTAC ID
non-CPTAC-6250
Peptide Molecular Mass
1,031.4325
Species
Human
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
SKBR3 cells
Submitting Laboratory
Cell Signaling Technology
Submitting Lab PI
Yiying Zhu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Orbitrap Q Exactive (Thermo)
Internal Standard
Heavy isotope-labeled peptides
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N F
LC
EASY-nLC II (Thermo)
Column Packing
self-packed
Column Dimensions
ID 100um, length 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 6.3 5.3 4.7 6 4.9 6 8.7 7.2 7.6 15 15 15
y7 (1+) 8.8 5.3 4 8.8 5.5 6.8 12.4 7.6 7.9 15 15 15
y7-98 (1+) 6.4 5 4.7 6 6 6.7 8.8 7.8 8.2 15 15 15
y6 (1+) 10.5 6.3 6.1 8.4 8.3 6 13.4 10.4 8.6 15 15 15
y6-98 (1+) 7 4.1 4 6.7 4.4 5.8 9.7 6 7 15 15 15
y7-98 (2+) 5.2 4.8 4.6 4.8 5.3 5.7 7.1 7.2 7.3 15 15 15
sum 6 4.5 4.3 5.4 4.9 5.8 8.1 6.7 7.2 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5333 Collapse assay details

Data source: Panorama

Official Gene Symbol
AKT1S1
Peptide Sequence
LNTSDFQK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
244
Peptide End
251
CPTAC ID
non-CPTAC-5333
Peptide Molecular Mass
951.4662
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N F
LC
RSLCnano
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 5 4.4 1.6 5 5.2 3.3 7.1 6.8 3.7 15 15 15
y7 (1+) 11.3 7.9 3.5 13.1 8.9 4.8 17.3 11.9 5.9 15 15 15
y4 (1+) 15 6.9 5.6 21 7.7 4.1 25.8 10.3 6.9 15 15 15
y5 (1+) 12.6 3.2 2.1 12.8 4.1 2.7 18 5.2 3.4 15 15 15
sum 4 3.1 1.4 4.7 4.3 3 6.2 5.3 3.3 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5698 Collapse assay details

Data source: Panorama

Official Gene Symbol
AKT1S1
Peptide Modified Sequence
LNT[+79.966331]SDFQK
Modification Type
Phospho (ST)
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
244
Peptide End
251
CPTAC ID
non-CPTAC-5698
Peptide Molecular Mass
1,031.4325
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Thermo Quantiva
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N F
LC
Dionex Ultimate 3000 RSLCnano
Column Packing
Pepmap 100
Column Dimensions
75um x 25cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7-98 (1+) 3.8 2.3 2.8 4.7 2.9 2.6 6 3.7 3.8 15 15 15
y6-98 (1+) 3.3 1.8 2.8 3.6 2.1 2.6 4.9 2.8 3.8 15 15 15
y5 (1+) 2.1 2.6 2.4 2.8 2.6 2.2 3.5 3.7 3.3 15 15 15
sum 2.2 1.7 2.2 2.8 1.9 2.1 3.6 2.5 3 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-785 Collapse assay details

Data source: Panorama

Official Gene Symbol
AKT1S1
Peptide Modified Sequence
C[+57.0]LHDIALAHR
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
61
Peptide - Site of Modification
1
Peptide Start
61
Peptide End
70
CPTAC ID
CPTAC-785
Peptide Molecular Mass
1,204.6135
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 17.8 9.8 7.2 24.4 9.7 12.3 30.2 13.8 14.3 15 15 15
y4 (1+) 30.7 15.5 7.5 27.9 11 13.9 41.5 19 15.8 15 15 15
b6 (1+) 32 23.5 12.9 33.3 27.1 14.6 46.2 35.9 19.5 15 15 15
b5 (1+) 48.8 16.3 16.1 43.9 18.6 16.7 65.6 24.7 23.2 15 15 15
b4 (1+) 19.3 10.3 9 17.7 10.9 10.1 26.2 15 13.5 15 15 15
b3 (1+) 29 17.5 18.4 27.5 21.6 18.2 40 27.8 25.9 15 15 15
sum 8.5 6.2 3.8 9.3 6 6.7 12.6 8.6 7.7 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-1038 Collapse assay details

Data source: Panorama

Official Gene Symbol
AKT1S1
Peptide Modified Sequence
LNT[+80.0]SDFQK
Modification Type
Phospho (ST)
Protein - Site of Modification
246
Peptide - Site of Modification
3
Peptide Start
244
Peptide End
251
CPTAC ID
CPTAC-1038
Peptide Molecular Mass
1,031.4325
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N F
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 21.7 15.1 10.6 28 15.1 11.2 35.4 21.4 15.4 15 15 15
y3 (1+) 12.9 5.6 8.4 15 7.1 6.6 19.8 9 10.7 15 15 15
y5 (1+) 30.8 11.1 8.4 25.9 11.3 10.8 40.2 15.8 13.7 15 15 15
sum 14.8 6.1 4.2 15.6 6.4 5.1 21.5 8.8 6.6 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-787 Collapse assay details

Data source: Panorama

Official Gene Symbol
AKT1S1
Peptide Sequence
SLPVSVPVWGFK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
183
Peptide End
194
CPTAC ID
CPTAC-787
Peptide Molecular Mass
1,314.7336
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 9.7 6.7 5 7.3 7 5.4 12.1 9.7 7.4 15 15 15
y4 (1+) 6.8 4.5 1.7 10.1 5.5 2.5 12.2 7.1 3 15 15 15
y6 (1+) 4.2 2.3 1.8 4.8 2.9 2.8 6.4 3.7 3.3 15 15 15
y7 (1+) 9.6 6.8 8.4 12.4 8.7 9 15.7 11 12.3 15 15 15
y8 (1+) 5.1 3.9 2.7 7.3 4.3 3.1 8.9 5.8 4.1 15 15 15
y9 (1+) 10.9 4.3 4.3 11.1 4.5 5.2 15.6 6.2 6.7 15 15 15
sum 1.8 1.3 1.1 2.4 2.1 1.9 3 2.5 2.2 15 15 15


Additional Resources and Comments