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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

C2

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
C2 N/A
Sequence Length (AA) Molecular Weight (Da)
752 83268
Protein Name
Complement C2
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGPLMVLFCL LFLYPGLADS APSCPQNVNI SGGTFTLSHG WAPGSLLTYS
60 70 80 90 100
CPQGLYPSPA SRLCKSSGQW QTPGATRSLS KAVCKPVRCP APVSFENGIY
110 120 130 140 150
TPRLGSYPVG GNVSFECEDG FILRGSPVRQ CRPNGMWDGE TAVCDNGAGH
160 170 180 190 200
CPNPGISLGA VRTGFRFGHG DKVRYRCSSN LVLTGSSERE CQGNGVWSGT
210 220 230 240 250
EPICRQPYSY DFPEDVAPAL GTSFSHMLGA TNPTQKTKES LGRKIQIQRS
260 270 280 290 300
GHLNLYLLLD CSQSVSENDF LIFKESASLM VDRIFSFEIN VSVAIITFAS
310 320 330 340 350
EPKVLMSVLN DNSRDMTEVI SSLENANYKD HENGTGTNTY AALNSVYLMM
360 370 380 390 400
NNQMRLLGME TMAWQEIRHA IILLTDGKSN MGGSPKTAVD HIREILNINQ
410 420 430 440 450
KRNDYLDIYA IGVGKLDVDW RELNELGSKK DGERHAFILQ DTKALHQVFE
460 470 480 490 500
HMLDVSKLTD TICGVGNMSA NASDQERTPW HVTIKPKSQE TCRGALISDQ
510 520 530 540 550
WVLTAAHCFR DGNDHSLWRV NVGDPKSQWG KEFLIEKAVI SPGFDVFAKK
560 570 580 590 600
NQGILEFYGD DIALLKLAQK VKMSTHARPI CLPCTMEANL ALRRPQGSTC
610 620 630 640 650
RDHENELLNK QSVPAHFVAL NGSKLNINLK MGVEWTSCAE VVSQEKTMFP
660 670 680 690 700
NLTDVREVVT DQFLCSGTQE DESPCKGESG GAVFLERRFR FFQVGLVSWG
710 720 730 740 750
LYNPCLGSAD KNSRKRAPRS KVPPPRDFHI NLFRMQPWLR QHLGDVLNFL
752
PL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-1104 Collapse assay details

Data source: Panorama

Official Gene Symbol
C2
Peptide Sequence
HAFILQDTK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
435
Peptide End
443
CPTAC ID
non-CPTAC-1104
Peptide Molecular Mass
1,071.5713
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b4 (1+) 10.2 7.4 5.8 8.7 8.7 4.7 13.4 11.4 7.5 15 15 15
b3 (1+) 10.7 8.9 6.8 14.6 7.7 7.4 18.1 11.8 10 15 15 15
y3 (1+) 24.9 9.4 6 25.4 8 6.5 35.6 12.3 8.8 15 15 15
y4 (1+) 11.7 6.9 4.4 6.6 7.8 7.7 13.4 10.4 8.9 5 15 15
y5 (1+) 20 12.1 3.7 22.1 12.7 5.8 29.8 17.5 6.9 15 15 15
sum 7.6 6.1 3.1 11.8 7 3.7 14 9.3 4.8 15 15 15

Additional Resources and Comments

Resources
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Assay Details for non-CPTAC-5589 Collapse assay details

Data source: Panorama

Official Gene Symbol
C2
Peptide Sequence
TGLNFDLGDK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
168
Peptide End
177
CPTAC ID
non-CPTAC-5589
Peptide Molecular Mass
1,078.5295
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (2+) 29.6 6.8 3.7 30.1 8.8 4.4 42.2 11.1 5.7 15 15 15
y3 (1+) 19.6 6.5 2.5 23.2 8 2.2 30.4 10.3 3.3 15 15 15
y4 (1+) 18.9 6 2.7 27 6.5 5.2 33 8.8 5.9 15 15 15
y6 (1+) 22.2 5.3 3.9 23.2 6.6 5.3 32.1 8.5 6.6 15 15 15
y7 (1+) 10.8 4.5 2.8 14.5 6.4 3.9 18.1 7.8 4.8 15 15 15
sum 10.6 2.3 1.2 13.3 5.5 2.7 17 6 3 15 15 15

Additional Resources and Comments

Resources
Download SOPs
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Comments

Assay Details for non-CPTAC-3777 Collapse assay details

Data source: Panorama

Official Gene Symbol
C2
Peptide Sequence
VPAHFVALNGNR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
622
Peptide End
633
CPTAC ID
non-CPTAC-3777
Peptide Molecular Mass
1,293.6942
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

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view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (3+) 46.2 66.8 65.8 60.5 76 161.9 76.1 101.2 174.8 15 15 15
y11 (3+) 8.5 4.9 7.8 11.3 8.7 7.9 14.1 10 11.1 15 15 15
y11 (2+) 4.5 5 3.9 7.5 11.1 7.1 8.7 12.2 8.1 15 15 15
y3 (1+) 5.8 6.5 5.5 9.3 8.9 8.2 11 11 9.9 15 15 15
y4 (1+) 4.6 3.5 3.3 8.3 7.7 5.2 9.5 8.5 6.2 15 15 15
sum 3 2.5 2.4 6.7 8.5 5.8 7.3 8.9 6.3 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments