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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

C2

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
C2 N/A
Sequence Length (AA) Molecular Weight (Da)
760 84741
Protein Name
Complement C2
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAPLLALFYL LQLGPGLAAL FCNQNVNITG GNFTLSHGWA PGSLLIYSCP
60 70 80 90 100
LGRYPSPAWR KCQSNGQWLT PRSSSHHTLR SSRMVKAVCK PVRCLAPSSF
110 120 130 140 150
ENGIYFPRLV SYPVGSNVSF ECEQDFTLRG SPVRYCRPNG LWDGETAVCD
160 170 180 190 200
NGASHCPNPG ISVGTARTGL NFDLGDKVRY RCSSSNMVLT GSAERECQSN
210 220 230 240 250
GVWSGSEPIC RQPYSYDFPE DVASALDTSL TNLLGATNPT QNLLTKSLGR
260 270 280 290 300
KIIIQRSGHL NLYLLLDASQ SVTEKDFDIF KKSAELMVER IFSFEVNVSV
310 320 330 340 350
AIITFASQPK TIMSILSERS QDVTEVITSL DSASYKDHEN ATGTNTYEVL
360 370 380 390 400
IRVYSMMQSQ MDRLGMETSA WKEIRHTIIL LTDGKSNMGD SPKKAVTRIR
410 420 430 440 450
ELLSIEQNRD DYLDIYAIGV GKLDVDWKEL NELGSKKDGE RHAFILQDAK
460 470 480 490 500
ALQQIFEHML DVSKLTDTIC GVGNMSANAS DQERTPWQVT FKPKSKETCQ
510 520 530 540 550
GSLISDQWVL TAAHCFHDIQ MEDHHLWRVN VGDPTSQHGK EFLVEDVIIA
560 570 580 590 600
PGFNVHAKRK QGISEFYADD IALLKLSRKV KMSTHARPIC LPCTVGANMA
610 620 630 640 650
LRRSPGSTCK DHETELLSQQ KVPAHFVALN GNRLNINLRT GPEWTRCIQA
660 670 680 690 700
VSQNKNIFPS LTNVSEVVTD QFLCSGMEEE DDNPCKGESG GAVFLGRRYR
710 720 730 740 750
FFQVGLVSWG LFDPCHGSSN KNLRKKPPRG VLPRDFHISL FRLQPWLRQH
760
LDGVLDFLPL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-1104 Collapse assay details

Data source: Panorama

Official Gene Symbol
C2
Peptide Sequence
HAFILQDTK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
435
Peptide End
443
CPTAC ID
non-CPTAC-1104
Peptide Molecular Mass
1,071.5713
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b4 (1+) 10.2 7.4 5.8 8.7 8.7 4.7 13.4 11.4 7.5 15 15 15
b3 (1+) 10.7 8.9 6.8 14.6 7.7 7.4 18.1 11.8 10 15 15 15
y3 (1+) 24.9 9.4 6 25.4 8 6.5 35.6 12.3 8.8 15 15 15
y4 (1+) 11.7 6.9 4.4 6.6 7.8 7.7 13.4 10.4 8.9 5 15 15
y5 (1+) 20 12.1 3.7 22.1 12.7 5.8 29.8 17.5 6.9 15 15 15
sum 7.6 6.1 3.1 11.8 7 3.7 14 9.3 4.8 15 15 15

Additional Resources and Comments

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Assay Details for non-CPTAC-5589 Collapse assay details

Data source: Panorama

Official Gene Symbol
C2
Peptide Sequence
TGLNFDLGDK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
168
Peptide End
177
CPTAC ID
non-CPTAC-5589
Peptide Molecular Mass
1,078.5295
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (2+) 29.6 6.8 3.7 30.1 8.8 4.4 42.2 11.1 5.7 15 15 15
y3 (1+) 19.6 6.5 2.5 23.2 8 2.2 30.4 10.3 3.3 15 15 15
y4 (1+) 18.9 6 2.7 27 6.5 5.2 33 8.8 5.9 15 15 15
y6 (1+) 22.2 5.3 3.9 23.2 6.6 5.3 32.1 8.5 6.6 15 15 15
y7 (1+) 10.8 4.5 2.8 14.5 6.4 3.9 18.1 7.8 4.8 15 15 15
sum 10.6 2.3 1.2 13.3 5.5 2.7 17 6 3 15 15 15

Additional Resources and Comments

Resources
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Comments

Assay Details for non-CPTAC-3777 Collapse assay details

Data source: Panorama

Official Gene Symbol
C2
Peptide Sequence
VPAHFVALNGNR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
622
Peptide End
633
CPTAC ID
non-CPTAC-3777
Peptide Molecular Mass
1,293.6942
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

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view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (3+) 46.2 66.8 65.8 60.5 76 161.9 76.1 101.2 174.8 15 15 15
y11 (3+) 8.5 4.9 7.8 11.3 8.7 7.9 14.1 10 11.1 15 15 15
y11 (2+) 4.5 5 3.9 7.5 11.1 7.1 8.7 12.2 8.1 15 15 15
y3 (1+) 5.8 6.5 5.5 9.3 8.9 8.2 11 11 9.9 15 15 15
y4 (1+) 4.6 3.5 3.3 8.3 7.7 5.2 9.5 8.5 6.2 15 15 15
sum 3 2.5 2.4 6.7 8.5 5.8 7.3 8.9 6.3 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments