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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

C5

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
C5 CPAMD4
Sequence Length (AA) Molecular Weight (Da)
1676 188305
Protein Name
Complement C5
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGLLGILCFL IFLGKTWGQE QTYVISAPKI FRVGASENIV IQVYGYTEAF
60 70 80 90 100
DATISIKSYP DKKFSYSSGH VHLSSENKFQ NSAILTIQPK QLPGGQNPVS
110 120 130 140 150
YVYLEVVSKH FSKSKRMPIT YDNGFLFIHT DKPVYTPDQS VKVRVYSLND
160 170 180 190 200
DLKPAKRETV LTFIDPEGSE VDMVEEIDHI GIISFPDFKI PSNPRYGMWT
210 220 230 240 250
IKAKYKEDFS TTGTAYFEVK EYVLPHFSVS IEPEYNFIGY KNFKNFEITI
260 270 280 290 300
KARYFYNKVV TEADVYITFG IREDLKDDQK EMMQTAMQNT MLINGIAQVT
310 320 330 340 350
FDSETAVKEL SYYSLEDLNN KYLYIAVTVI ESTGGFSEEA EIPGIKYVLS
360 370 380 390 400
PYKLNLVATP LFLKPGIPYP IKVQVKDSLD QLVGGVPVTL NAQTIDVNQE
410 420 430 440 450
TSDLDPSKSV TRVDDGVASF VLNLPSGVTV LEFNVKTDAP DLPEENQARE
460 470 480 490 500
GYRAIAYSSL SQSYLYIDWT DNHKALLVGE HLNIIVTPKS PYIDKITHYN
510 520 530 540 550
YLILSKGKII HFGTREKFSD ASYQSINIPV TQNMVPSSRL LVYYIVTGEQ
560 570 580 590 600
TAELVSDSVW LNIEEKCGNQ LQVHLSPDAD AYSPGQTVSL NMATGMDSWV
610 620 630 640 650
ALAAVDSAVY GVQRGAKKPL ERVFQFLEKS DLGCGAGGGL NNANVFHLAG
660 670 680 690 700
LTFLTNANAD DSQENDEPCK EILRPRRTLQ KKIEEIAAKY KHSVVKKCCY
710 720 730 740 750
DGACVNNDET CEQRAARISL GPRCIKAFTE CCVVASQLRA NISHKDMQLG
760 770 780 790 800
RLHMKTLLPV SKPEIRSYFP ESWLWEVHLV PRRKQLQFAL PDSLTTWEIQ
810 820 830 840 850
GVGISNTGIC VADTVKAKVF KDVFLEMNIP YSVVRGEQIQ LKGTVYNYRT
860 870 880 890 900
SGMQFCVKMS AVEGICTSES PVIDHQGTKS SKCVRQKVEG SSSHLVTFTV
910 920 930 940 950
LPLEIGLHNI NFSLETWFGK EILVKTLRVV PEGVKRESYS GVTLDPRGIY
960 970 980 990 1000
GTISRRKEFP YRIPLDLVPK TEIKRILSVK GLLVGEILSA VLSQEGINIL
1010 1020 1030 1040 1050
THLPKGSAEA ELMSVVPVFY VFHYLETGNH WNIFHSDPLI EKQKLKKKLK
1060 1070 1080 1090 1100
EGMLSIMSYR NADYSYSVWK GGSASTWLTA FALRVLGQVN KYVEQNQNSI
1110 1120 1130 1140 1150
CNSLLWLVEN YQLDNGSFKE NSQYQPIKLQ GTLPVEAREN SLYLTAFTVI
1160 1170 1180 1190 1200
GIRKAFDICP LVKIDTALIK ADNFLLENTL PAQSTFTLAI SAYALSLGDK
1210 1220 1230 1240 1250
THPQFRSIVS ALKREALVKG NPPIYRFWKD NLQHKDSSVP NTGTARMVET
1260 1270 1280 1290 1300
TAYALLTSLN LKDINYVNPV IKWLSEEQRY GGGFYSTQDT INAIEGLTEY
1310 1320 1330 1340 1350
SLLVKQLRLS MDIDVSYKHK GALHNYKMTD KNFLGRPVEV LLNDDLIVST
1360 1370 1380 1390 1400
GFGSGLATVH VTTVVHKTST SEEVCSFYLK IDTQDIEASH YRGYGNSDYK
1410 1420 1430 1440 1450
RIVACASYKP SREESSSGSS HAVMDISLPT GISANEEDLK ALVEGVDQLF
1460 1470 1480 1490 1500
TDYQIKDGHV ILQLNSIPSS DFLCVRFRIF ELFEVGFLSP ATFTVYEYHR
1510 1520 1530 1540 1550
PDKQCTMFYS TSNIKIQKVC EGAACKCVEA DCGQMQEELD LTISAETRKQ
1560 1570 1580 1590 1600
TACKPEIAYA YKVSITSITV ENVFVKYKAT LLDIYKTGEA VAEKDSEITF
1610 1620 1630 1640 1650
IKKVTCTNAE LVKGRQYLIM GKEALQIKYN FSFRYIYPLD SLTWIEYWPR
1660 1670 1676
DTTCSSCQAF LANLDEFAED IFLNGC

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-1108 Collapse assay details

Data source: Panorama

Official Gene Symbol
C5
Peptide Sequence
FQNSAILTIQPK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
79
Peptide End
90
CPTAC ID
non-CPTAC-1108
Peptide Molecular Mass
1,358.7558
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 19.2 6.4 4.5 19.2 8.1 4.6 27.2 10.3 6.4 14 15 15
y2 (1+) 36.8 7.3 5.1 35.5 9.3 6 51.1 11.8 7.9 13 15 15
y6 (1+) 21 12.2 9.8 22.9 12.6 7.3 31.1 17.5 12.2 15 15 15
y7 (1+) 28.4 7.4 5.6 34.5 11.3 8.5 44.7 13.5 10.2 15 15 15
y10 (1+) 30.7 6.4 4.2 28.5 8.7 7 41.9 10.8 8.2 15 15 15
sum 11.1 4 3.3 12 6.2 3.7 16.3 7.4 5 15 15 15

Additional Resources and Comments

Resources
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Assay Details for non-CPTAC-3698 Collapse assay details

Data source: Panorama

Official Gene Symbol
C5
Peptide Sequence
IDTQDIEASSHFR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1385
Peptide End
1397
CPTAC ID
non-CPTAC-3698
Peptide Molecular Mass
1,517.7110
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (2+) 17.3 7.7 4 16.9 8.6 8.2 24.2 11.5 9.1 15 15 15
y11 (2+) 13 4 4.5 13.6 8.5 8.8 18.8 9.4 9.9 15 15 15
y12 (2+) 11.6 4.3 4.9 12 9.3 5.5 16.7 10.2 7.4 15 15 15
y6 (1+) 16.2 4.1 6.4 15.5 7.3 10.7 22.4 8.4 12.5 15 15 15
y7 (1+) 22.5 7.1 7.4 22.2 10.8 16.5 31.6 12.9 18.1 15 15 15
sum 7.9 2.4 3 8 7.6 6.6 11.2 8 7.2 15 15 15

Additional Resources and Comments

Resources
Download SOPs
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View Protein in Panorama
Comments

Assay Details for non-CPTAC-1109 Collapse assay details

Data source: Panorama

Official Gene Symbol
C5
Peptide Sequence
VFQFLEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
623
Peptide End
629
CPTAC ID
non-CPTAC-1109
Peptide Molecular Mass
909.4960
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

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view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 18.3 6.7 7.2 19.8 7.4 6.5 27 10 9.7 15 15 15
y2 (1+) 21.4 8.8 8.5 24.4 9.2 7.4 32.5 12.7 11.3 10 15 15
y4 (1+) 16.3 7.8 5 15.2 7 5.3 22.3 10.5 7.3 15 15 15
y5 (1+) 14.1 5.6 3.6 15.9 5.3 4.6 21.3 7.7 5.8 15 15 15
y6 (1+) 15.1 5.9 6.4 17.4 7.1 7.6 23 9.2 9.9 15 15 15
sum 16.4 4.1 3 16.1 3.6 3 23 5.5 4.2 15 15 15

Additional Resources and Comments

Resources
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View Protein in Panorama
Comments

Assay Details for non-CPTAC-4025 Collapse assay details

Data source: Panorama

Official Gene Symbol
C5
Peptide Sequence
VTFSSGYVNLSPENK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
64
Peptide End
78
CPTAC ID
non-CPTAC-4025
Peptide Molecular Mass
1,640.8046
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 6.6 5.9 5.1 7.8 8.1 5.9 10.2 10 7.8 15 15 15
y5 (1+) 6.8 3.5 3.8 10.3 4.5 4.7 12.3 5.7 6 15 15 15
y4 (1+) 5.1 2.4 4.7 7.2 5.4 4.4 8.8 5.9 6.4 15 15 15
y2 (1+) 10.1 9 4.2 9.7 15 13.3 14 17.5 13.9 15 15 15
b2 (1+) 14.2 7.5 7.1 15.6 14.2 15.3 21.1 16.1 16.9 15 15 15
sum 3.6 1.7 3.2 6.2 4.8 4 7.2 5.1 5.1 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments