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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

KRT2

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
KRT2 KRT2A, KRT2E
Sequence Length (AA) Molecular Weight (Da)
639 65433
Protein Name
Keratin, type II cytoskeletal 2 epidermal
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSCQISCKSR GRGGGGGGFR GFSSGSAVVS GGSRRSTSSF SCLSRHGGGG
60 70 80 90 100
GGFGGGGFGS RSLVGLGGTK SISISVAGGG GGFGAAGGFG GRGGGFGGGS
110 120 130 140 150
SFGGGSGFSG GGFGGGGFGG GRFGGFGGPG GVGGLGGPGG FGPGGYPGGI
160 170 180 190 200
HEVSVNQSLL QPLNVKVDPE IQNVKAQERE QIKTLNNKFA SFIDKVRFLE
210 220 230 240 250
QQNQVLQTKW ELLQQMNVGT RPINLEPIFQ GYIDSLKRYL DGLTAERTSQ
260 270 280 290 300
NSELNNMQDL VEDYKKKYED EINKRTAAEN DFVTLKKDVD NAYMIKVELQ
310 320 330 340 350
SKVDLLNQEI EFLKVLYDAE ISQIHQSVTD TNVILSMDNS RNLDLDSIIA
360 370 380 390 400
EVKAQYEEIA QRSKEEAEAL YHSKYEELQV TVGRHGDSLK EIKIEISELN
410 420 430 440 450
RVIQRLQGEI AHVKKQCKNV QDAIADAEQR GEHALKDARN KLNDLEEALQ
460 470 480 490 500
QAKEDLARLL RDYQELMNVK LALDVEIATY RKLLEGEECR MSGDLSSNVT
510 520 530 540 550
VSVTSSTISS NVASKAAFGG SGGRGSSSGG GYSSGSSSYG SGGRQSGSRG
560 570 580 590 600
GSGGGGSISG GGYGSGGGSG GRYGSGGGSK GGSISGGGYG SGGGKHSSGG
610 620 630 639
GSRGGSSSGG GYGSGGGGSS SVKGSSGEAF GSSVTFSFR

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3922 Collapse assay details

Data source: Panorama

Official Gene Symbol
Krt2
Peptide Sequence
VDPEIQNVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
188
Peptide End
196
CPTAC ID
non-CPTAC-3922
Peptide Molecular Mass
1,040.5502
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 15.8 7.6 5.2 24.7 8.6 5.8 29.3 11.5 7.8 15 15 15
y7 (2+) 9.2 4.7 3.7 14 10 7.7 16.8 11 8.5 15 15 15
y8 (2+) 29 20.2 10.1 39.2 19.8 12.1 48.8 28.3 15.8 15 15 15
y6 (1+) 41.6 9.7 8 40.3 11.9 9.1 57.9 15.4 12.1 15 15 15
y7 (1+) 22.4 8.4 6.3 28 9.7 10.8 35.9 12.8 12.5 15 15 15
sum 7.6 4.4 3.1 11.6 9.1 7.1 13.9 10.1 7.7 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-1137 Collapse assay details

Data source: Panorama

Official Gene Symbol
KRT2
Peptide Sequence
YEELQVTVGR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
375
Peptide End
384
CPTAC ID
non-CPTAC-1137
Peptide Molecular Mass
1,192.6088
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 12.5 7.1 12.4 14.6 23.4 13.9 19.2 24.5 18.6 15 15 15
y4 (1+) 14.4 6.4 13.5 13.8 24.6 15 19.9 25.4 20.2 15 15 15
y5 (1+) 12.3 7.8 16.1 13.7 21.4 16.1 18.4 22.8 22.8 15 15 15
y6 (1+) 13.5 11.6 13 15.8 27.3 14.4 20.8 29.7 19.4 15 15 15
y8 (1+) 10.4 5.8 14.1 11.9 25.1 14.1 15.8 25.8 19.9 15 15 15
sum 10.9 5.9 12.8 11.5 24 13.6 15.8 24.7 18.7 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments