Krt2, CPTAC-3922 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Krt2	K2e, Krt2-17, Krt2a

Sequence Length (AA)	Molecular Weight (Da)
707	70923

Protein Name
Keratin, type II cytoskeletal 2 epidermal

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSCQISCRSR	RGGGGGGGGG	FRGFSSGSAV	VSGGSRRSNT	SFSCISRHGG
60	70	80	90	100
GRGGSGGGGF	GSQSLVGLGG	YKSISSSVAG	NSGGYGGSSF	GGSSGFGGGR
110	120	130	140	150
GFGGGQGFGG	SGGFGGGSGF	GGGQGFGGGS	RFGGGSGFGG	GGFGGGSFGG
160	170	180	190	200
GRFGGGPGGF	GGPGGFPGGG	IHEVSVNQSL	LQPLDVKVDP	EIQNVKSQER
210	220	230	240	250
EQIKTLNNKF	ASFIDKVRFL	EQQNQVLRTK	WELLQQLDVG	SRTTNLDPIF
260	270	280	290	300
QAYIGMLKKQ	VDRLSAERTS	QESELNNMQD	LVEDFKKKYE	DEINKRTSAE
310	320	330	340	350
NDFVTIKKDV	DSCYMDKTEL	QARLDILAQE	VNFLRTLYDA	ELSQLQQDVT
360	370	380	390	400
DTNVILSMDN	NRNLDLDSII	AEVQNQYEMI	AHKSKAESEE	LYHSKYEELQ
410	420	430	440	450
VTAVKHGDSL	KEIKMEISEL	NRTIQRLQGE	ISHVKKQCKG	VQDSIADAEQ
460	470	480	490	500
RGEHAIKDAR	GKLTDLEEAL	QQCREDLARL	LRDYQELMNT	KLSLDVEIAT
510	520	530	540	550
YRKLLEGEEC	RMSGDFSDNV	SVSITSSTIS	SSVASKTGFG	SGGQSSGGRG
560	570	580	590	600
SYGGRGGGGG	GGSTYGSGGR	SSGSRGSGSG	SGGGGYSSGG	GSRGGSGGGY
610	620	630	640	650
GSGGGSRGGS	GGGYGSGGGS	GSGGGYSSGG	GSRGGSGGGG	VSSGGGSRGG
660	670	680	690	700
SSSGGGSRGG	SSSGGGGYSS	GGGSRGGSSS	GGAGSSSEKG	GSGSGEGCGS
707
GVTFSFR

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for non-CPTAC-3922 Collapse assay details

Data source: Panorama

Official Gene Symbol: Krt2
Peptide Sequence: VDPEIQNVK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 188
Peptide End: 196

CPTAC ID: non-CPTAC-3922
Peptide Molecular Mass: 1,040.5502
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >80%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b2 (1+)	15.8	7.6	5.2	24.7	8.6	5.8	29.3	11.5	7.8	15	15	15
y7 (2+)	9.2	4.7	3.7	14	10	7.7	16.8	11	8.5	15	15	15
y8 (2+)	29	20.2	10.1	39.2	19.8	12.1	48.8	28.3	15.8	15	15	15
y6 (1+)	41.6	9.7	8	40.3	11.9	9.1	57.9	15.4	12.1	15	15	15
y7 (1+)	22.4	8.4	6.3	28	9.7	10.8	35.9	12.8	12.5	15	15	15
sum	7.6	4.4	3.1	11.6	9.1	7.1	13.9	10.1	7.7	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-1137 Collapse assay details

Data source: Panorama

Official Gene Symbol: KRT2
Peptide Sequence: YEELQVTVGR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 375
Peptide End: 384

CPTAC ID: non-CPTAC-1137
Peptide Molecular Mass: 1,192.6088
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers