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Overview Data source: UniProt

Official Gene Symbol Other Aliases
PON3 N/A
Sequence Length (AA) Molecular Weight (Da)
354 39607
Protein Name
Serum paraoxonase/lactonase 3
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGKLVALVLL GVGLSLVGEM FLAFRERVNA SREVEPVEPE NCHLIEELES
60 70 80 90 100
GSEDIDILPS GLAFISSGLK YPGMPNFAPD EPGKIFLMDL NEQNPRAQAL
110 120 130 140 150
EISGGFDKEL FNPHGISIFI DKDNTVYLYV VNHPHMKSTV EIFKFEEQQR
160 170 180 190 200
SLVYLKTIKH ELLKSVNDIV VLGPEQFYAT RDHYFTNSLL SFFEMILDLR
210 220 230 240 250
WTYVLFYSPR EVKVVAKGFC SANGITVSAD QKYVYVADVA AKNIHIMEKH
260 270 280 290 300
DNWDLTQLKV IQLGTLVDNL TVDPATGDIL AGCHPNPMKL LNYNPEDPPG
310 320 330 340 350
SEVLRIQNVL SEKPRVSTVY ANNGSVLQGT SVASVYHGKI LIGTVFHKTL
354
YCEL

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3939 Collapse assay details

Data source: Panorama

Official Gene Symbol
Pon3
Peptide Sequence
FVYVADVTAK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
233
Peptide End
242
CPTAC ID
non-CPTAC-3939
Peptide Molecular Mass
1,111.5914
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 5.7 3.5 3.1 4.9 7.4 4.3 7.5 8.2 5.3 15 15 15
y7 (1+) 9.1 5.9 3.9 9.9 8.1 8 13.4 10 8.9 15 15 15
y6 (1+) 7 4.2 4 8.3 8.7 7 10.9 9.7 8.1 15 15 15
b2 (1+) 4.2 2.8 1.4 5.5 7.1 4.5 6.9 7.6 4.7 15 15 15
b3 (1+) 12.7 3.8 3.3 15.4 6.5 5.7 20 7.5 6.6 15 15 15
sum 2.4 1.1 1.4 2.9 6.6 4.6 3.8 6.7 4.8 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-2696 Collapse assay details

Data source: Panorama

Official Gene Symbol
PON3
Peptide Sequence
IQNVLSEKPR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
306
Peptide End
315
CPTAC ID
non-CPTAC-2696
Peptide Molecular Mass
1,182.6721
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (2+) 13.5 4.1 6.2 14.6 10.7 9.5 19.9 11.5 11.3 15 15 15
y9 (2+) 18 8.9 6.9 22.4 9.2 13.5 28.7 12.8 15.2 15 15 15
y3 (1+) 31.3 6.1 8.8 34.1 7.7 10.2 46.3 9.8 13.5 15 15 15
y6 (1+) 14.7 6 8.1 21.5 7.8 9.1 26 9.8 12.2 15 15 15
y5 (1+) 15.4 9.6 5.7 16.7 9.5 10.4 22.7 13.5 11.9 15 15 15
sum 9.6 3.2 4.9 10.6 7.4 8.6 14.3 8.1 9.9 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-2697 Collapse assay details

Data source: Panorama

Official Gene Symbol
PON3
Peptide Sequence
LLNYNPEDPPGSEVLR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
290
Peptide End
305
CPTAC ID
non-CPTAC-2697
Peptide Molecular Mass
1,811.9054
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b8 (1+) 11.3 6.3 6.1 16.7 5.9 9 20.2 8.6 10.9 15 15 15
y8 (2+) 6.8 4.1 6.9 17.4 6.2 10.7 18.7 7.4 12.7 15 15 15
y8 (1+) 11.4 5.9 7.1 16.9 7.9 9.4 20.4 9.9 11.8 15 15 15
y11 (2+) 14.2 8.8 5.5 17.3 10.6 8.1 22.4 13.8 9.8 15 15 15
y7 (1+) 8 6.2 7.6 11.9 8.2 10.7 14.3 10.3 13.1 15 15 15
sum 4.1 3.7 5.6 14.1 6 8.6 14.7 7 10.3 15 15 15

Additional Resources and Comments