PON3, CPTAC-2697 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
PON3	N/A

Sequence Length (AA)	Molecular Weight (Da)
354	39607

Protein Name
Serum paraoxonase/lactonase 3

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MGKLVALVLL	GVGLSLVGEM	FLAFRERVNA	SREVEPVEPE	NCHLIEELES
60	70	80	90	100
GSEDIDILPS	GLAFISSGLK	YPGMPNFAPD	EPGKIFLMDL	NEQNPRAQAL
110	120	130	140	150
EISGGFDKEL	FNPHGISIFI	DKDNTVYLYV	VNHPHMKSTV	EIFKFEEQQR
160	170	180	190	200
SLVYLKTIKH	ELLKSVNDIV	VLGPEQFYAT	RDHYFTNSLL	SFFEMILDLR
210	220	230	240	250
WTYVLFYSPR	EVKVVAKGFC	SANGITVSAD	QKYVYVADVA	AKNIHIMEKH
260	270	280	290	300
DNWDLTQLKV	IQLGTLVDNL	TVDPATGDIL	AGCHPNPMKL	LNYNPEDPPG
310	320	330	340	350
SEVLRIQNVL	SEKPRVSTVY	ANNGSVLQGT	SVASVYHGKI	LIGTVFHKTL
354
YCEL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3939 Collapse assay details

Data source: Panorama

Official Gene Symbol: Pon3
Peptide Sequence: FVYVADVTAK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 233
Peptide End: 242

CPTAC ID: non-CPTAC-3939
Peptide Molecular Mass: 1,111.5914
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >80%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8 (1+)	5.7	3.5	3.1	4.9	7.4	4.3	7.5	8.2	5.3	15	15	15
y7 (1+)	9.1	5.9	3.9	9.9	8.1	8	13.4	10	8.9	15	15	15
y6 (1+)	7	4.2	4	8.3	8.7	7	10.9	9.7	8.1	15	15	15
b2 (1+)	4.2	2.8	1.4	5.5	7.1	4.5	6.9	7.6	4.7	15	15	15
b3 (1+)	12.7	3.8	3.3	15.4	6.5	5.7	20	7.5	6.6	15	15	15
sum	2.4	1.1	1.4	2.9	6.6	4.6	3.8	6.7	4.8	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-2696 Collapse assay details

Data source: Panorama

Official Gene Symbol: PON3
Peptide Sequence: IQNVLSEKPR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 306
Peptide End: 315

CPTAC ID: non-CPTAC-2696
Peptide Molecular Mass: 1,182.6721
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers