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Overview Data source: UniProt

Official Gene Symbol Other Aliases
Col3a1 N/A
Sequence Length (AA) Molecular Weight (Da)
1464 138943
Protein Name
Collagen alpha-1(III) chain
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MMSFVQSGTW FLLTLLHPTL ILAQQSNVDE LGCSHLGQSY ESRDVWKPEP
60 70 80 90 100
CQICVCDSGS VLCDDIICDE EPLDCPNPEI PFGECCAICP QPSTPAPVLP
110 120 130 140 150
DGHGPQGPKG DPGPPGIPGR NGDPGLPGQP GLPGPPGSPG ICESCPTGGQ
160 170 180 190 200
NYSPQFDSYD VKSGVGGMGG YPGPAGPPGP PGPPGSSGHP GSPGSPGYQG
210 220 230 240 250
PPGEPGQAGP AGPPGPPGAL GPAGPAGKDG ESGRPGRPGE RGLPGPPGIK
260 270 280 290 300
GPAGMPGFPG MKGHRGFDGR NGEKGETGAP GLKGENGLPG DNGAPGPMGP
310 320 330 340 350
RGAPGERGRP GLPGAAGARG NDGARGSDGQ PGPPGPPGTA GFPGSPGAKG
360 370 380 390 400
EVGPAGSPGS NGSPGQRGEP GPQGHAGAQG PPGPPGNNGS PGGKGEMGPA
410 420 430 440 450
GIPGAPGLIG ARGPPGPAGT NGIPGTRGPS GEPGKNGAKG EPGARGERGE
460 470 480 490 500
AGSPGIPGPK GEDGKDGSPG EPGANGLPGA AGERGPSGFR GPAGPNGIPG
510 520 530 540 550
EKGPPGERGG PGPAGPRGVA GEPGRDGTPG GPGIRGMPGS PGGPGNDGKP
560 570 580 590 600
GPPGSQGESG RPGPPGPSGP RGQPGVMGFP GPKGNDGAPG KNGERGGPGG
610 620 630 640 650
PGLPGPAGKN GETGPQGPPG PTGPAGDKGD SGPPGPQGLQ GIPGTGGPPG
660 670 680 690 700
ENGKPGEPGP KGEVGAPGAP GGKGDSGAPG ERGPPGTAGI PGARGGAGPP
710 720 730 740 750
GPEGGKGPAG PPGPPGASGS PGLQGMPGER GGPGSPGPKG EKGEPGGAGA
760 770 780 790 800
DGVPGKDGPR GPAGPIGPPG PAGQPGDKGE GGSPGLPGIA GPRGGPGERG
810 820 830 840 850
EHGPPGPAGF PGAPGQNGEP GAKGERGAPG EKGEGGPPGP AGPTGSSGPA
860 870 880 890 900
GPPGPQGVKG ERGSPGGPGT AGFPGGRGLP GPPGNNGNPG PPGPSGAPGK
910 920 930 940 950
DGPPGPAGNS GSPGNPGIAG PKGDAGQPGE KGPPGAQGPP GSPGPLGIAG
960 970 980 990 1000
LTGARGLAGP PGMPGPRGSP GPQGIKGESG KPGASGHNGE RGPPGPQGLP
1010 1020 1030 1040 1050
GQPGTAGEPG RDGNPGSDGQ PGRDGSPGGK GDRGENGSPG APGAPGHPGP
1060 1070 1080 1090 1100
PGPVGPSGKS GDRGETGPAG PSGAPGPAGA RGAPGPQGPR GDKGETGERG
1110 1120 1130 1140 1150
SNGIKGHRGF PGNPGPPGSP GAAGHQGAIG SPGPAGPRGP VGPHGPPGKD
1160 1170 1180 1190 1200
GTSGHPGPIG PPGPRGNRGE RGSEGSPGHP GQPGPPGPPG APGPCCGGGA
1210 1220 1230 1240 1250
AAIAGVGGEK SGGFSPYYGD DPMDFKINTE EIMSSLKSVN GQIESLISPD
1260 1270 1280 1290 1300
GSRKNPARNC RDLKFCHPEL KSGEYWVDPN QGCKMDAIKV FCNMETGETC
1310 1320 1330 1340 1350
INASPMTVPR KHWWTDSGAE KKHVWFGESM NGGFQFSYGP PDLPEDVVDV
1360 1370 1380 1390 1400
QLAFLRLLSS RASQNITYHC KNSIAYMDQA SGNVKKSLKL MGSNEGEFKA
1410 1420 1430 1440 1450
EGNSKFTYTV LEDGCTKHTG EWSKTVFEYQ TRKAMRLPII DIAPYDIGGP
1460 1464
DQEFGVDIGP VCFL

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1180 Collapse assay details

Data source: Panorama

Official Gene Symbol
COL3A1
Peptide Sequence
GPVGPSGPPGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1140
Peptide End
1150
CPTAC ID
CPTAC-1180
Peptide Molecular Mass
948.5029
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 29 13 14.2 27.5 15.8 14.8 40 20.5 20.5 15 15 15
y7 (1+) 22.9 7.4 9.6 22 15.1 11.7 31.8 16.8 15.1 15 15 15
y8 (1+) 13.1 4.9 8.9 16.2 8.5 8.9 20.8 9.8 12.6 15 15 15
sum 8.9 5.1 6.4 9.2 9.3 7.5 12.8 10.6 9.9 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-3364 Collapse assay details

Data source: Panorama

Official Gene Symbol
Col3a1
Peptide Sequence
GGAGPPGPEGGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
695
Peptide End
706
CPTAC ID
non-CPTAC-3364
Peptide Molecular Mass
979.4723
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b4 (1+) 10.6 12.3 11.6 16.4 14.7 13.6 19.5 19.2 17.9 15 15 15
y8 (2+) 7.3 4.9 6.4 13.5 10.1 10.1 15.3 11.2 12 15 15 15
y9 (2+) 15.6 10.6 6.3 21.5 14.3 10 26.6 17.8 11.8 15 15 15
y7 (1+) 9.6 8.4 8.6 20.1 13.1 9.4 22.3 15.6 12.7 15 15 15
y8 (1+) 9.7 8.5 7.1 16.2 15.6 9.9 18.9 17.8 12.2 15 15 15
sum 4.4 4.2 4.5 14.9 10.5 7.8 15.5 11.3 9 15 15 15

Additional Resources and Comments