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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Mpo

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Mpo N/A
Sequence Length (AA) Molecular Weight (Da)
718 81182
Protein Name
Myeloperoxidase
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MKLLLALAGL LAPLAMLQTS NGATPALLGE VENSVVLSCM EEAKQLVDRA
60 70 80 90 100
YKERRESIKR SLQSGSASPT ELLFYFKQPV AGTRTAVRAA DYLHVALDLL
110 120 130 140 150
KRKLQPLWPR PFNVTDVLTP AQLNLLSVSS GCAYQDVRVT CPPNDKYRTI
160 170 180 190 200
TGHCNNRRSP TLGASNRAFV RWLPAEYEDG VSMPFGWTPG VNRNGFKVPL
210 220 230 240 250
ARQVSNAIVR FPNDQLTKDQ ERALMFMQWG QFLDHDITLT PEPATRFSFF
260 270 280 290 300
TGLNCETSCL QQPPCFPLKI PPNDPRIKNQ KDCIPFFRSC PACTRNNITI
310 320 330 340 350
RNQINALTSF VDASGVYGSE DPLARKLRNL TNQLGLLAIN TRFQDNGRAL
360 370 380 390 400
MPFDSLHDDP CLLTNRSARI PCFLAGDMRS SEMPELTSMH TLFVREHNRL
410 420 430 440 450
ATQLKRLNPR WNGEKLYQEA RKIVGAMVQI ITYRDYLPLV LGPAAMKKYL
460 470 480 490 500
PQYRSYNDSV DPRIANVFTN AFRYGHTLIQ PFMFRLNNQY RPTGPNPRVP
510 520 530 540 550
LSKVFFASWR VVLEGGIDPI LRGLMATPAK LNRQNQIVVD EIRERLFEQV
560 570 580 590 600
MRIGLDLPAL NMQRSRDHGL PGYNAWRRFC GLPQPSTVGE LGTVLKNLEL
610 620 630 640 650
ARKLMAQYGT PNNIDIWMGG VSEPLEPNGR VGQLLACLIG TQFRKLRDGD
660 670 680 690 700
RFWWENPGVF SKQQRQALAS ISLPRIICDN TGITTVSKNN IFMSNTYPRD
710 718
FVSCNTLPKL NLTSWKET

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-6037 Collapse assay details

Data source: Panorama

Official Gene Symbol
MPO
Peptide Modified Sequence
IGLDLPALNM[+15.994915]QR
Modification Type
Oxidation (M)
Protein - Site of Modification
588
Peptide - Site of Modification
10
Peptide Start
579
Peptide End
590
CPTAC ID
CPTAC-6037
Peptide Molecular Mass
1,355.7231
Species
Human
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
FFPE lung tumor tissue lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Whiteaker JR, Zhao L, Schoenherr RM, Huang D, Lundeen RA, Voytovich U, Kennedy JJ, Ivey RG, Lin C, Murillo OD, Lorentzen TD, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Reading JJ, Perry CD, Richardson CW, Garcia-Buntley SS, Bocik W, Hewitt SM, Chowdhury S, Vandermeer J, Smith SD, Gopal AK, Ramchurren N, Fling SP, Wang P, Paulovich AG. A multiplexed assay for quantifying immunomodulatory proteins supports correlative studies in immunotherapy clinical trials. Front Oncol. 2023 May 2;13:1168710. doi: 10.3389/fonc.2023.1168710. PMID: 37205196; PMCID: PMC10185886.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTRAP 5500
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Eksigent nanoLC 425
Column Packing
3 µm ChromXP C18EP, 120 Å
Column Dimensions
150 x 0.075 mm
Flow Rate
0.3 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b4 (1+) 61 67.6 40.5 70.7 86.3 59.5 93.4 109.6 72 15 15 15
b5 (1+) 41.8 60.7 58.9 69.1 59.7 75.7 80.8 85.1 95.9 15 15 15
y4 (1+) 78.4 71.2 58.6 84.2 72.7 72.7 115 101.8 93.4 15 15 15
y6 (1+) 72.4 70.4 53.7 79.1 87.5 76.1 107.2 112.3 93.1 15 15 15
y7 (1+) 42.9 62.4 33.3 42.4 74.9 40.6 60.3 97.5 52.5 15 15 15
y8 (1+) 48.6 68.8 31.2 45.1 67.7 74 66.3 96.5 80.3 15 15 15
y9 (1+) 54 85.5 44.5 56.4 83.4 64.3 78.1 119.4 78.2 15 15 15
sum 34.9 62 32.4 32 71.4 48.1 47.3 94.6 58 15 15 15

Additional Resources and Comments

Resources
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Assay Details for CPTAC-6053 Collapse assay details

Data source: Panorama

Official Gene Symbol
MPO
Peptide Sequence
IGLDLPALNMQR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
579
Peptide End
590
CPTAC ID
CPTAC-6053
Peptide Molecular Mass
1,339.7282
Species
Human
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
FFPE lung tumor tissue lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Whiteaker JR, Zhao L, Schoenherr RM, Huang D, Lundeen RA, Voytovich U, Kennedy JJ, Ivey RG, Lin C, Murillo OD, Lorentzen TD, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Reading JJ, Perry CD, Richardson CW, Garcia-Buntley SS, Bocik W, Hewitt SM, Chowdhury S, Vandermeer J, Smith SD, Gopal AK, Ramchurren N, Fling SP, Wang P, Paulovich AG. A multiplexed assay for quantifying immunomodulatory proteins supports correlative studies in immunotherapy clinical trials. Front Oncol. 2023 May 2;13:1168710. doi: 10.3389/fonc.2023.1168710. PMID: 37205196; PMCID: PMC10185886.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTRAP 5500
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Eksigent nanoLC 425
Column Packing
3 µm ChromXP C18EP, 120 Å
Column Dimensions
150 x 0.075 mm
Flow Rate
0.3 µL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

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view larger image

Retrieving Data

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Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b5 (1+) 20.5 20.8 19.8 18.4 22.7 23.4 27.5 30.8 30.7 15 15 15
y7 (1+) 11 24.3 17.1 12.2 24.3 17.9 16.4 34.4 24.8 15 15 15
y8 (1+) 9.3 22.3 20.4 16.2 26 27.3 18.7 34.3 34.1 15 15 15
y9 (1+) 17.9 24.9 24.6 18.4 29 25.9 25.7 38.2 35.7 15 15 15
sum 10.7 23.8 16.9 12 24.1 18 16.1 33.9 24.7 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-3375 Collapse assay details

Data source: Panorama

Official Gene Symbol
Mpo
Peptide Sequence
IANVFTNAFR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
464
Peptide End
473
CPTAC ID
non-CPTAC-3375
Peptide Molecular Mass
1,151.6087
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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view larger image

Response Curves

Data source: Panorama

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view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b4 (1+) 11.9 6.6 3.9 19.1 10.3 4.9 22.5 12.2 6.3 15 15 15
b3 (1+) 11.7 4 5.8 26.9 12.8 7.1 29.3 13.4 9.2 15 15 15
y5 (1+) 3.5 6.5 6 13.7 11.3 6.8 14.1 13 9.1 15 15 15
y6 (1+) 5.8 6.7 3.6 13.6 11.7 4.7 14.8 13.5 5.9 15 15 15
y8 (1+) 5.5 5.2 5.7 13.3 7.9 6.2 14.4 9.5 8.4 15 15 15
sum 2.6 3 1.5 14.4 9.5 3.8 14.6 10 4.1 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments