Krt17, CPTAC-3467 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Krt17	Krt1-17

Sequence Length (AA)	Molecular Weight (Da)
433	48162

Protein Name
Keratin, type I cytoskeletal 17

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MTTTIRQFTS	SSSIKGSSGL	GGGSSRTSCR	LSGSLGAGSC	RLGSASGLGS
60	70	80	90	100
ALGSNSYSSC	YSFGTGSGYG	GNFGGVDGLL	AGGEKATMQN	LNDRLASYLD
110	120	130	140	150
KVRALEEANT	ELEVKIRDWY	QKQAPGPARD	YSAYYHTIED	LKNKILVATV
160	170	180	190	200
DNASILLQID	NARLAADDFR	TKFETEQALR	MSVEADINGL	RRVLDELTLA
210	220	230	240	250
RADLEMQIEN	LKEELAYLKK	NHEEEMNALR	GQVGGEINVE	MDAAPGVDLS
260	270	280	290	300
RILSEMRDQY	EKMAEKNRKD	AEDWFFSKTE	ELNREVATNS	ELVQSGKSEI
310	320	330	340	350
SELRRTMQAL	EIELQSQLSM	KASLEGSLAE	TENRYCVQLS	QIQGLIGSVE
360	370	380	390	400
EQLAQLRCEM	EQQNQEYKIL	LDVKTRLEQE	IATYRRLLEG	EDAHLTQYKP
410	420	430	433
KEPVTTRQVR	TIVEEVQDGK	VISSREQVHQ	TTR

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1516 Collapse assay details

Data source: Panorama

Official Gene Symbol: KRT17
Peptide Sequence: ASLEGNLAETENR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 322
Peptide End: 334

CPTAC ID: CPTAC-1516
Peptide Molecular Mass: 1,402.6688
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Waters nanoACQUITY (Part Number 176016000)
Column Packing: Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions: 100 um x 100 mm
Flow Rate: 0.4 uL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y4 (1+)	12.3	10.6	4.6	36.3	24.8	18.6	38.3	27	19.2	15	15	15
y5 (1+)	8.9	7.3	5.1	35	29.2	27.5	36.1	30.1	28	15	15	15
y6 (1+)	5.9	7.3	4.9	35.7	28.1	27.9	36.2	29	28.3	15	15	15
y8 (1+)	17.3	9.8	5.2	40.6	29.9	25	44.1	31.5	25.5	15	15	15
sum	5	7.4	4.5	35.2	27.6	25.4	35.6	28.6	25.8	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-1517 Collapse assay details

Data source: Panorama

Official Gene Symbol: KRT17
Peptide Sequence: TIVEEVQDGK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 410
Peptide End: 419

CPTAC ID: CPTAC-1517
Peptide Molecular Mass: 1,116.5663
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu