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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
KRT17 N/A
Sequence Length (AA) Molecular Weight (Da)
432 48106
Protein Name
Keratin, type I cytoskeletal 17
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MTTSIRQFTS SSSIKGSSGL GGGSSRTSCR LSGGLGAGSC RLGSAGGLGS
60 70 80 90 100
TLGGSSYSSC YSFGSGGGYG SSFGGVDGLL AGGEKATMQN LNDRLASYLD
110 120 130 140 150
KVRALEEANT ELEVKIRDWY QRQAPGPARD YSQYYRTIEE LQNKILTATV
160 170 180 190 200
DNANILLQID NARLAADDFR TKFETEQALR LSVEADINGL RRVLDELTLA
210 220 230 240 250
RADLEMQIEN LKEELAYLKK NHEEEMNALR GQVGGEINVE MDAAPGVDLS
260 270 280 290 300
RILNEMRDQY EKMAEKNRKD AEDWFFSKTE ELNREVATNS ELVQSGKSEI
310 320 330 340 350
SELRRTMQAL EIELQSQLSM KASLEGNLAE TENRYCVQLS QIQGLIGSVE
360 370 380 390 400
EQLAQLRCEM EQQNQEYKIL LDVKTRLEQE IATYRRLLEG EDAHLTQYKK
410 420 430 432
EPVTTRQVRT IVEEVQDGKV ISSREQVHQT TR

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1516 Collapse assay details

Data source: Panorama

Official Gene Symbol
KRT17
Peptide Sequence
ASLEGNLAETENR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
322
Peptide End
334
CPTAC ID
CPTAC-1516
Peptide Molecular Mass
1,402.6688
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.4 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM1


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 12.3 10.6 4.6 36.3 24.8 18.6 38.3 27 19.2 15 15 15
y5 (1+) 8.9 7.3 5.1 35 29.2 27.5 36.1 30.1 28 15 15 15
y6 (1+) 5.9 7.3 4.9 35.7 28.1 27.9 36.2 29 28.3 15 15 15
y8 (1+) 17.3 9.8 5.2 40.6 29.9 25 44.1 31.5 25.5 15 15 15
sum 5 7.4 4.5 35.2 27.6 25.4 35.6 28.6 25.8 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-1517 Collapse assay details

Data source: Panorama

Official Gene Symbol
KRT17
Peptide Sequence
TIVEEVQDGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
410
Peptide End
419
CPTAC ID
CPTAC-1517
Peptide Molecular Mass
1,116.5663
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.4 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM1


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 10.1 5.7 2.4 33.4 28.2 27.7 34.9 28.8 27.8 15 15 15
y7 (1+) 7.8 6.1 2.3 32.8 29.2 27.9 33.7 29.8 28 15 15 15
y8 (1+) 5.5 4.9 1.5 37.2 27.8 27.4 37.6 28.2 27.4 15 15 15
sum 4.5 4.7 1.4 35.5 28 27.5 35.8 28.4 27.5 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-3467 Collapse assay details

Data source: Panorama

Official Gene Symbol
Krt17
Peptide Sequence
ASLEGSLAETENR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
322
Peptide End
334
CPTAC ID
non-CPTAC-3467
Peptide Molecular Mass
1,375.6579
Species
Mus musculus (Mouse)
Assay Type
Direct MRM or SRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM1


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y11 (2+) 26.8 13.5 13.8 28 17.2 17.2 38.8 21.9 22.1 15 15 15
y5 (1+) 10.6 13.6 13.2 14 11.9 15 17.6 18.1 20 15 15 15
y6 (1+) 16.2 12.3 8.9 17.8 16.4 13.1 24.1 20.5 15.8 15 15 15
y7 (1+) 26.6 18.3 12 35.4 22 16.7 44.3 28.6 20.6 15 15 15
y9 (1+) 10.8 8.5 8.8 10.5 9.9 9.8 15.1 13 13.2 15 15 15
sum 7.3 7.5 6.4 9.5 8.9 8.6 12 11.6 10.7 15 15 15


Additional Resources and Comments