AP1S2, CPTAC-1535 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
AP1S2	N/A

Sequence Length (AA)	Molecular Weight (Da)
157	18615

Protein Name
AP-1 complex subunit sigma-2

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MQFMLLFSRQ	GKLRLQKWYV	PLSDKEKKKI	TRELVQTVLA	RKPKMCSFLE
60	70	80	90	100
WRDLKIVYKR	YASLYFCCAI	EDQDNELITL	EIIHRYVELL	DKYFGSVCEL
110	120	130	140	150
DIIFNFEKAY	FILDEFLLGG	EVQETSKKNV	LKAIEQADLL	QEEAETPRSV
157
LEEIGLT

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for CPTAC-1535 Collapse assay details

Data source: Panorama

Official Gene Symbol: AP1S2
Peptide Sequence: ELVQTVLAR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 33
Peptide End: 41

CPTAC ID: CPTAC-1535
Peptide Molecular Mass: 1,027.6026
Species: Homo sapiens (Human)
Assay Type: Direct MRM or SRM
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Waters nanoACQUITY (Part Number 176016000)
Column Packing: Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions: 100 um x 100 mm
Flow Rate: 0.4 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM1

CPTAC-1516: KRT17. ASLEGNLAETENR	CPTAC-1517: KRT17. TIVEEVQDGK	CPTAC-1518: KRT18. SLGSVQAPSYGAR
CPTAC-1519: KRT18. AQIFANTVDNAR	CPTAC-1520: KRT7. SAYGGPVGAGIR	CPTAC-1521: KRT7. SLDLDGIIAEVK
CPTAC-1522: KRT19. ALEAANGELEVK	CPTAC-1523: KRT19. AALEDTLAETEAR	CPTAC-1524: KRT8. YEELQSLAGK
CPTAC-1525: KRT8. LVSESSDVLPK	CPTAC-1526: OVGP1. ILYPEFNK	CPTAC-1527: OVGP1. THDFDGLDLFFLYPGLR
CPTAC-1528: OLFM4. VQSINYNPFDQK	CPTAC-1529: OLFM4. LYVYNDGYLLNYDLSVLQKPQ	CPTAC-1530: GNAI2. LLLLGAGESGK
CPTAC-1531: GNAI2. IAQSDYIPTQQDVLR	CPTAC-1532: PTMS. SVEAAAELSAK	CPTAC-1533: PTMS. AAEEEDEADPKR
CPTAC-1534: AP1S2. WYVPLSDK	CPTAC-1535: AP1S2. ELVQTVLAR	CPTAC-1536: ME3. VLTSDVEK
CPTAC-1537: ME3. GIFASGSPFK

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y5 (1+)	9.4	6	2.9	44	35.1	32.6	45	35.6	32.7	15	15	15
y6 (1+)	4.7	7.1	2.7	43	33.9	32.4	43.3	34.6	32.5	15	15	15
y7 (1+)	10.9	6.2	2.3	40.5	31.9	30.7	41.9	32.5	30.8	15	15	15
sum	4.5	5.9	1.7	42.6	33.9	32	42.8	34.4	32	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-1534 Collapse assay details

Data source: Panorama

Official Gene Symbol: AP1S2
Peptide Sequence: WYVPLSDK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 18
Peptide End: 25

CPTAC ID: CPTAC-1534
Peptide Molecular Mass: 1,006.5124
Species: Homo sapiens (Human)
Assay Type: Direct MRM or SRM
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu