KRT19, CPTAC-1523 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
KRT19	N/A

Sequence Length (AA)	Molecular Weight (Da)
400	44106

Protein Name
Keratin, type I cytoskeletal 19

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MTSYSYRQSS	ATSSFGGLGG	GSVRFGPGVA	FRAPSIHGGS	GGRGVSVSSA
60	70	80	90	100
RFVSSSSSGA	YGGGYGGVLT	ASDGLLAGNE	KLTMQNLNDR	LASYLDKVRA
110	120	130	140	150
LEAANGELEV	KIRDWYQKQG	PGPSRDYSHY	YTTIQDLRDK	ILGATIENSR
160	170	180	190	200
IVLQIDNARL	AADDFRTKFE	TEQALRMSVE	ADINGLRRVL	DELTLARTDL
210	220	230	240	250
EMQIEGLKEE	LAYLKKNHEE	EISTLRGQVG	GQVSVEVDSA	PGTDLAKILS
260	270	280	290	300
DMRSQYEVMA	EQNRKDAEAW	FTSRTEELNR	EVAGHTEQLQ	MSRSEVTDLR
310	320	330	340	350
RTLQGLEIEL	QSQLSMKAAL	EDTLAETEAR	FGAQLAHIQA	LISGIEAQLG
360	370	380	390	400
DVRADSERQN	QEYQRLMDIK	SRLEQEIATY	RSLLEGQEDH	YNNLSASKVL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1523 Collapse assay details

Data source: Panorama

Official Gene Symbol: KRT19
Peptide Sequence: AALEDTLAETEAR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 318
Peptide End: 330

CPTAC ID: CPTAC-1523
Peptide Molecular Mass: 1,388.6783
Species: Homo sapiens (Human)
Assay Type: Direct MRM or SRM
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Waters nanoACQUITY (Part Number 176016000)
Column Packing: Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions: 100 um x 100 mm
Flow Rate: 0.4 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM1

CPTAC-1516: KRT17. ASLEGNLAETENR	CPTAC-1517: KRT17. TIVEEVQDGK	CPTAC-1518: KRT18. SLGSVQAPSYGAR
CPTAC-1519: KRT18. AQIFANTVDNAR	CPTAC-1520: KRT7. SAYGGPVGAGIR	CPTAC-1521: KRT7. SLDLDGIIAEVK
CPTAC-1522: KRT19. ALEAANGELEVK	CPTAC-1523: KRT19. AALEDTLAETEAR	CPTAC-1524: KRT8. YEELQSLAGK
CPTAC-1525: KRT8. LVSESSDVLPK	CPTAC-1526: OVGP1. ILYPEFNK	CPTAC-1527: OVGP1. THDFDGLDLFFLYPGLR
CPTAC-1528: OLFM4. VQSINYNPFDQK	CPTAC-1529: OLFM4. LYVYNDGYLLNYDLSVLQKPQ	CPTAC-1530: GNAI2. LLLLGAGESGK
CPTAC-1531: GNAI2. IAQSDYIPTQQDVLR	CPTAC-1532: PTMS. SVEAAAELSAK	CPTAC-1533: PTMS. AAEEEDEADPKR
CPTAC-1534: AP1S2. WYVPLSDK	CPTAC-1535: AP1S2. ELVQTVLAR	CPTAC-1536: ME3. VLTSDVEK
CPTAC-1537: ME3. GIFASGSPFK

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y4 (1+)	59.2	10.7	5.6	55	31.5	35.6	80.8	33.3	36	15	15	15
y5 (1+)	38.7	14.6	5.1	37.1	28.6	30.9	53.6	32.1	31.3	15	15	15
y6 (1+)	35.9	13	5.2	43.7	27.2	31.6	56.6	30.1	32	15	15	15
sum	30.7	8.2	3.4	37.7	27	32.1	48.6	28.2	32.3	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for CPTAC-1522 Collapse assay details

Data source: Panorama

Official Gene Symbol: KRT19
Peptide Sequence: ALEAANGELEVK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 100
Peptide End: 111

CPTAC ID: CPTAC-1522
Peptide Molecular Mass: 1,242.6456
Species: Homo sapiens (Human)
Assay Type: Direct MRM or SRM
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu