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Overview Data source: UniProt

Official Gene Symbol Other Aliases
ME3 N/A
Sequence Length (AA) Molecular Weight (Da)
604 67068
Protein Name
NADP-dependent malic enzyme, mitochondrial
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGAALGTGTR LAPWPGRACG ALPRWTPTAP AQGCHSKPGP ARPVPLKKRG
60 70 80 90 100
YDVTRNPHLN KGMAFTLEER LQLGIHGLIP PCFLSQDVQL LRIMRYYERQ
110 120 130 140 150
QSDLDKYIIL MTLQDRNEKL FYRVLTSDVE KFMPIVYTPT VGLACQHYGL
160 170 180 190 200
TFRRPRGLFI TIHDKGHLAT MLNSWPEDNI KAVVVTDGER ILGLGDLGCY
210 220 230 240 250
GMGIPVGKLA LYTACGGVNP QQCLPVLLDV GTNNEELLRD PLYIGLKHQR
260 270 280 290 300
VHGKAYDDLL DEFMQAVTDK FGINCLIQFE DFANANAFRL LNKYRNKYCM
310 320 330 340 350
FNDDIQGTAS VAVAGILAAL RITKNKLSNH VFVFQGAGEA AMGIAHLLVM
360 370 380 390 400
ALEKEGVPKA EATRKIWMVD SKGLIVKGRS HLNHEKEMFA QDHPEVNSLE
410 420 430 440 450
EVVRLVKPTA IIGVAAIAGA FTEQILRDMA SFHERPIIFA LSNPTSKAEC
460 470 480 490 500
TAEKCYRVTE GRGIFASGSP FKSVTLEDGK TFIPGQGNNA YVFPGVALGV
510 520 530 540 550
IAGGIRHIPD EIFLLTAEQI AQEVSEQHLS QGRLYPPLST IRDVSLRIAI
560 570 580 590 600
KVLDYAYKHN LASYYPEPKD KEAFVRSLVY TPDYDSFTLD SYTWPKEAMN
604
VQTV

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1537 Collapse assay details

Data source: Panorama

Official Gene Symbol
ME3
Peptide Sequence
GIFASGSPFK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
463
Peptide End
472
CPTAC ID
CPTAC-1537
Peptide Molecular Mass
1,009.5233
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.4 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM1


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (2+) 8.4 6.1 5.6 20.8 29.4 23.6 22.4 30 24.3 15 15 15
y3 (1+) 11.3 6.7 4.4 25.2 31.1 26.8 27.6 31.8 27.2 15 15 15
y6 (1+) 3.2 7 2.4 25.1 30 23.8 25.3 30.8 23.9 15 15 15
y7 (1+) 5.8 6.6 4.1 23.4 32.4 25.2 24.1 33.1 25.5 15 15 15
sum 3.4 5.4 2.3 22.5 30.5 24.4 22.8 31 24.5 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-1536 Collapse assay details

Data source: Panorama

Official Gene Symbol
ME3
Peptide Sequence
VLTSDVEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
124
Peptide End
131
CPTAC ID
CPTAC-1536
Peptide Molecular Mass
889.4757
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.4 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM1


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 4.8 4.1 3.9 26.3 17.2 7.1 26.7 17.7 8.1 15 15 15
y4 (1+) 11.8 4.2 4.6 23.9 16.3 9.9 26.7 16.8 10.9 15 15 15
y5 (1+) 6.5 3.6 5.2 21.6 13.8 6.8 22.6 14.3 8.6 15 15 15
sum 4.5 2.3 3.3 22.6 15.4 6.9 23 15.6 7.6 15 15 15


Additional Resources and Comments