ME3, CPTAC-1537 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
ME3	N/A

Sequence Length (AA)	Molecular Weight (Da)
604	67068

Protein Name
NADP-dependent malic enzyme, mitochondrial

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MGAALGTGTR	LAPWPGRACG	ALPRWTPTAP	AQGCHSKPGP	ARPVPLKKRG
60	70	80	90	100
YDVTRNPHLN	KGMAFTLEER	LQLGIHGLIP	PCFLSQDVQL	LRIMRYYERQ
110	120	130	140	150
QSDLDKYIIL	MTLQDRNEKL	FYRVLTSDVE	KFMPIVYTPT	VGLACQHYGL
160	170	180	190	200
TFRRPRGLFI	TIHDKGHLAT	MLNSWPEDNI	KAVVVTDGER	ILGLGDLGCY
210	220	230	240	250
GMGIPVGKLA	LYTACGGVNP	QQCLPVLLDV	GTNNEELLRD	PLYIGLKHQR
260	270	280	290	300
VHGKAYDDLL	DEFMQAVTDK	FGINCLIQFE	DFANANAFRL	LNKYRNKYCM
310	320	330	340	350
FNDDIQGTAS	VAVAGILAAL	RITKNKLSNH	VFVFQGAGEA	AMGIAHLLVM
360	370	380	390	400
ALEKEGVPKA	EATRKIWMVD	SKGLIVKGRS	HLNHEKEMFA	QDHPEVNSLE
410	420	430	440	450
EVVRLVKPTA	IIGVAAIAGA	FTEQILRDMA	SFHERPIIFA	LSNPTSKAEC
460	470	480	490	500
TAEKCYRVTE	GRGIFASGSP	FKSVTLEDGK	TFIPGQGNNA	YVFPGVALGV
510	520	530	540	550
IAGGIRHIPD	EIFLLTAEQI	AQEVSEQHLS	QGRLYPPLST	IRDVSLRIAI
560	570	580	590	600
KVLDYAYKHN	LASYYPEPKD	KEAFVRSLVY	TPDYDSFTLD	SYTWPKEAMN
604
VQTV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-1537 Collapse assay details

Data source: Panorama

Official Gene Symbol: ME3
Peptide Sequence: GIFASGSPFK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 463
Peptide End: 472

CPTAC ID: CPTAC-1537
Peptide Molecular Mass: 1,009.5233
Species: Homo sapiens (Human)
Assay Type: Direct MRM or SRM
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Waters nanoACQUITY (Part Number 176016000)
Column Packing: Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions: 100 um x 100 mm
Flow Rate: 0.4 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM1

CPTAC-1516: KRT17. ASLEGNLAETENR	CPTAC-1517: KRT17. TIVEEVQDGK	CPTAC-1518: KRT18. SLGSVQAPSYGAR
CPTAC-1519: KRT18. AQIFANTVDNAR	CPTAC-1520: KRT7. SAYGGPVGAGIR	CPTAC-1521: KRT7. SLDLDGIIAEVK
CPTAC-1522: KRT19. ALEAANGELEVK	CPTAC-1523: KRT19. AALEDTLAETEAR	CPTAC-1524: KRT8. YEELQSLAGK
CPTAC-1525: KRT8. LVSESSDVLPK	CPTAC-1526: OVGP1. ILYPEFNK	CPTAC-1527: OVGP1. THDFDGLDLFFLYPGLR
CPTAC-1528: OLFM4. VQSINYNPFDQK	CPTAC-1529: OLFM4. LYVYNDGYLLNYDLSVLQKPQ	CPTAC-1530: GNAI2. LLLLGAGESGK
CPTAC-1531: GNAI2. IAQSDYIPTQQDVLR	CPTAC-1532: PTMS. SVEAAAELSAK	CPTAC-1533: PTMS. AAEEEDEADPKR
CPTAC-1534: AP1S2. WYVPLSDK	CPTAC-1535: AP1S2. ELVQTVLAR	CPTAC-1536: ME3. VLTSDVEK
CPTAC-1537: ME3. GIFASGSPFK

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8 (2+)	8.4	6.1	5.6	20.8	29.4	23.6	22.4	30	24.3	15	15	15
y3 (1+)	11.3	6.7	4.4	25.2	31.1	26.8	27.6	31.8	27.2	15	15	15
y6 (1+)	3.2	7	2.4	25.1	30	23.8	25.3	30.8	23.9	15	15	15
y7 (1+)	5.8	6.6	4.1	23.4	32.4	25.2	24.1	33.1	25.5	15	15	15
sum	3.4	5.4	2.3	22.5	30.5	24.4	22.8	31	24.5	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-1536 Collapse assay details

Data source: Panorama

Official Gene Symbol: ME3
Peptide Sequence: VLTSDVEK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 124
Peptide End: 131

CPTAC ID: CPTAC-1536
Peptide Molecular Mass: 889.4757
Species: Homo sapiens (Human)
Assay Type: Direct MRM or SRM
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu