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Overview Data source: UniProt

Official Gene Symbol Other Aliases
KRT8 CYK8
Sequence Length (AA) Molecular Weight (Da)
483 53704
Protein Name
Keratin, type II cytoskeletal 8
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSIRVTQKSY KVSTSGPRAF SSRSYTSGPG SRISSSSFSR VGSSNFRGGL
60 70 80 90 100
GGGYGGASGM GGITAVTVNQ SLLSPLVLEV DPNIQAVRTQ EKEQIKTLNN
110 120 130 140 150
KFASFIDKVR FLEQQNKMLE TKWSLLQQQK TARSNMDNMF ESYINNLRRQ
160 170 180 190 200
LETLGQEKLK LEAELGNMQG LVEDFKNKYE DEINKRTEME NEFVLIKKDV
210 220 230 240 250
DEAYMNKVEL ESRLEGLTDE INFLRQLYEE EIRELQSQIS DTSVVLSMDN
260 270 280 290 300
SRSLDMDSII AEVKAQYEDI ANRSRAEAES MYQIKYEELQ SLAGKHGDDL
310 320 330 340 350
RRTKTEISEM NRNISRLQAE IEGLKGQRAS LEAAIADAEQ RGELAIKDAN
360 370 380 390 400
AKLSELEAAL QRAKQDMARQ LREYQELMNV KLALDIEIAT YRKLLEGEES
410 420 430 440 450
RLESGMQNMS IHTKTTSGYA GGLSSAYGGL TSPGLSYSLG SSFGSGAGSS
460 470 480 483
SFSRTSSSRA VVVKKIETRD GKLVSESSDV LPK

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1525 Collapse assay details

Data source: Panorama

Official Gene Symbol
KRT8
Peptide Sequence
LVSESSDVLPK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
473
Peptide End
483
CPTAC ID
CPTAC-1525
Peptide Molecular Mass
1,172.6289
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.4 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM1


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 55.3 16.1 12.8 44.9 36.2 35.1 71.2 39.6 37.4 15 15 15
y7 (1+) 29.2 14.7 5.8 32.9 34.8 33.7 44 37.8 34.2 15 15 15
y8 (1+) 25.6 19 10.6 32.1 32.3 36.2 41.1 37.5 37.7 15 15 15
y9 (1+) 17.7 10.6 2.5 40.1 31.4 33.2 43.8 33.1 33.3 15 15 15
sum 9.4 8.2 2.7 29.4 31.3 33.3 30.9 32.4 33.4 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-1524 Collapse assay details

Data source: Panorama

Official Gene Symbol
KRT8
Peptide Sequence
YEELQSLAGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
286
Peptide End
295
CPTAC ID
CPTAC-1524
Peptide Molecular Mass
1,136.5714
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.4 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM1


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 46.3 13.8 5.8 54.1 24.5 33.7 71.2 28.1 34.2 15 15 15
y6 (1+) 40.3 15 7.8 65.2 29.2 34.1 76.6 32.8 35 15 15 15
y7 (1+) 61.4 11.1 6.4 76.3 29.8 34.2 97.9 31.8 34.8 15 15 15
y8 (1+) 33.4 9.9 2.9 50 30 31.5 60.1 31.6 31.6 15 15 15
sum 21.1 8.1 2.8 44 28.2 32.5 48.8 29.3 32.6 15 15 15


Additional Resources and Comments