Show Nav →

Overview Data source: UniProt

Official Gene Symbol Other Aliases
KRT19 N/A
Sequence Length (AA) Molecular Weight (Da)
400 44106
Protein Name
Keratin, type I cytoskeletal 19
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MTSYSYRQSS ATSSFGGLGG GSVRFGPGVA FRAPSIHGGS GGRGVSVSSA
60 70 80 90 100
RFVSSSSSGA YGGGYGGVLT ASDGLLAGNE KLTMQNLNDR LASYLDKVRA
110 120 130 140 150
LEAANGELEV KIRDWYQKQG PGPSRDYSHY YTTIQDLRDK ILGATIENSR
160 170 180 190 200
IVLQIDNARL AADDFRTKFE TEQALRMSVE ADINGLRRVL DELTLARTDL
210 220 230 240 250
EMQIEGLKEE LAYLKKNHEE EISTLRGQVG GQVSVEVDSA PGTDLAKILS
260 270 280 290 300
DMRSQYEVMA EQNRKDAEAW FTSRTEELNR EVAGHTEQLQ MSRSEVTDLR
310 320 330 340 350
RTLQGLEIEL QSQLSMKAAL EDTLAETEAR FGAQLAHIQA LISGIEAQLG
360 370 380 390 400
DVRADSERQN QEYQRLMDIK SRLEQEIATY RSLLEGQEDH YNNLSASKVL

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for CPTAC-1523 Collapse assay details

Data source: Panorama

Official Gene Symbol
KRT19
Peptide Sequence
AALEDTLAETEAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
318
Peptide End
330
CPTAC ID
CPTAC-1523
Peptide Molecular Mass
1,388.6783
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.4 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM1


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 59.2 10.7 5.6 55 31.5 35.6 80.8 33.3 36 15 15 15
y5 (1+) 38.7 14.6 5.1 37.1 28.6 30.9 53.6 32.1 31.3 15 15 15
y6 (1+) 35.9 13 5.2 43.7 27.2 31.6 56.6 30.1 32 15 15 15
sum 30.7 8.2 3.4 37.7 27 32.1 48.6 28.2 32.3 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-1522 Collapse assay details

Data source: Panorama

Official Gene Symbol
KRT19
Peptide Sequence
ALEAANGELEVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
100
Peptide End
111
CPTAC ID
CPTAC-1522
Peptide Molecular Mass
1,242.6456
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.4 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM1


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 11.7 9.8 4.5 45.9 21.4 32.8 47.4 23.5 33.1 15 15 15
y7 (1+) 13.1 10.5 3.7 40.7 22.5 30.7 42.8 24.8 30.9 15 15 15
y8 (1+) 11.8 8.7 2.7 39.6 20.3 31.7 41.3 22.1 31.8 15 15 15
y10 (1+) 6.6 9.8 2.7 45.4 22.4 30.4 45.9 24.4 30.5 15 15 15
sum 7.1 8.6 1.6 41.6 21.2 31.1 42.2 22.9 31.1 15 15 15

Additional Resources and Comments