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Overview Data source: UniProt

Official Gene Symbol Other Aliases
GNAI2 GNAI2B
Sequence Length (AA) Molecular Weight (Da)
355 40451
Protein Name
Guanine nucleotide-binding protein G(i) subunit alpha-2
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGCTVSAEDK AAAERSKMID KNLREDGEKA AREVKLLLLG AGESGKSTIV
60 70 80 90 100
KQMKIIHEDG YSEEECRQYR AVVYSNTIQS IMAIVKAMGN LQIDFADPSR
110 120 130 140 150
ADDARQLFAL SCTAEEQGVL PDDLSGVIRR LWADHGVQAC FGRSREYQLN
160 170 180 190 200
DSAAYYLNDL ERIAQSDYIP TQQDVLRTRV KTTGIVETHF TFKDLHFKMF
210 220 230 240 250
DVGGQRSERK KWIHCFEGVT AIIFCVALSA YDLVLAEDEE MNRMHESMKL
260 270 280 290 300
FDSICNNKWF TDTSIILFLN KKDLFEEKIT HSPLTICFPE YTGANKYDEA
310 320 330 340 350
ASYIQSKFED LNKRKDTKEI YTHFTCATDT KNVQFVFDAV TDVIIKNNLK
355
DCGLF

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1531 Collapse assay details

Data source: Panorama

Official Gene Symbol
GNAI2
Peptide Sequence
IAQSDYIPTQQDVLR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
163
Peptide End
177
CPTAC ID
CPTAC-1531
Peptide Molecular Mass
1,745.8948
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.4 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM1


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b6 (1+) 15.7 10 7.1 53.8 25.6 31.5 56 27.5 32.3 15 15 15
y8 (1+) 6.3 6.9 3.4 56 24.9 31.4 56.4 25.8 31.6 15 15 15
y9 (1+) 12.7 9.9 5.2 57.4 23.7 32.6 58.8 25.7 33 15 15 15
y10 (1+) 23.1 8.8 4.6 58.9 24.5 30.3 63.3 26 30.6 15 15 15
sum 4.5 6.5 2.8 55.9 24.6 31.3 56.1 25.4 31.4 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-1530 Collapse assay details

Data source: Panorama

Official Gene Symbol
GNAI2
Peptide Sequence
LLLLGAGESGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
36
Peptide End
46
CPTAC ID
CPTAC-1530
Peptide Molecular Mass
1,056.6179
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.4 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM1


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 3.2 2.4 1.8 42.1 26.6 26.8 42.2 26.7 26.9 15 15 15
y8 (1+) 6.5 2.6 1.1 41 26.3 26.6 41.5 26.4 26.6 15 15 15
y9 (1+) 2.9 2.5 1.6 39.9 25.3 26.8 40 25.4 26.8 15 15 15
sum 2.9 1.9 0.8 41 26.1 26.7 41.1 26.2 26.7 15 15 15


Additional Resources and Comments