GNAI2, CPTAC-1531 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
GNAI2	GNAI2B

Sequence Length (AA)	Molecular Weight (Da)
355	40451

Protein Name
Guanine nucleotide-binding protein G(i) subunit alpha-2

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MGCTVSAEDK	AAAERSKMID	KNLREDGEKA	AREVKLLLLG	AGESGKSTIV
60	70	80	90	100
KQMKIIHEDG	YSEEECRQYR	AVVYSNTIQS	IMAIVKAMGN	LQIDFADPSR
110	120	130	140	150
ADDARQLFAL	SCTAEEQGVL	PDDLSGVIRR	LWADHGVQAC	FGRSREYQLN
160	170	180	190	200
DSAAYYLNDL	ERIAQSDYIP	TQQDVLRTRV	KTTGIVETHF	TFKDLHFKMF
210	220	230	240	250
DVGGQRSERK	KWIHCFEGVT	AIIFCVALSA	YDLVLAEDEE	MNRMHESMKL
260	270	280	290	300
FDSICNNKWF	TDTSIILFLN	KKDLFEEKIT	HSPLTICFPE	YTGANKYDEA
310	320	330	340	350
ASYIQSKFED	LNKRKDTKEI	YTHFTCATDT	KNVQFVFDAV	TDVIIKNNLK
355
DCGLF

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1531 Collapse assay details

Data source: Panorama

Official Gene Symbol: GNAI2
Peptide Sequence: IAQSDYIPTQQDVLR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 163
Peptide End: 177

CPTAC ID: CPTAC-1531
Peptide Molecular Mass: 1,745.8948
Species: Homo sapiens (Human)
Assay Type: Direct MRM or SRM
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Waters nanoACQUITY (Part Number 176016000)
Column Packing: Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions: 100 um x 100 mm
Flow Rate: 0.4 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM1

CPTAC-1516: KRT17. ASLEGNLAETENR	CPTAC-1517: KRT17. TIVEEVQDGK	CPTAC-1518: KRT18. SLGSVQAPSYGAR
CPTAC-1519: KRT18. AQIFANTVDNAR	CPTAC-1520: KRT7. SAYGGPVGAGIR	CPTAC-1521: KRT7. SLDLDGIIAEVK
CPTAC-1522: KRT19. ALEAANGELEVK	CPTAC-1523: KRT19. AALEDTLAETEAR	CPTAC-1524: KRT8. YEELQSLAGK
CPTAC-1525: KRT8. LVSESSDVLPK	CPTAC-1526: OVGP1. ILYPEFNK	CPTAC-1527: OVGP1. THDFDGLDLFFLYPGLR
CPTAC-1528: OLFM4. VQSINYNPFDQK	CPTAC-1529: OLFM4. LYVYNDGYLLNYDLSVLQKPQ	CPTAC-1530: GNAI2. LLLLGAGESGK
CPTAC-1531: GNAI2. IAQSDYIPTQQDVLR	CPTAC-1532: PTMS. SVEAAAELSAK	CPTAC-1533: PTMS. AAEEEDEADPKR
CPTAC-1534: AP1S2. WYVPLSDK	CPTAC-1535: AP1S2. ELVQTVLAR	CPTAC-1536: ME3. VLTSDVEK
CPTAC-1537: ME3. GIFASGSPFK

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b6 (1+)	15.7	10	7.1	53.8	25.6	31.5	56	27.5	32.3	15	15	15
y8 (1+)	6.3	6.9	3.4	56	24.9	31.4	56.4	25.8	31.6	15	15	15
y9 (1+)	12.7	9.9	5.2	57.4	23.7	32.6	58.8	25.7	33	15	15	15
y10 (1+)	23.1	8.8	4.6	58.9	24.5	30.3	63.3	26	30.6	15	15	15
sum	4.5	6.5	2.8	55.9	24.6	31.3	56.1	25.4	31.4	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-1530 Collapse assay details

Data source: Panorama

Official Gene Symbol: GNAI2
Peptide Sequence: LLLLGAGESGK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 36
Peptide End: 46

CPTAC ID: CPTAC-1530
Peptide Molecular Mass: 1,056.6179
Species: Homo sapiens (Human)
Assay Type: Direct MRM or SRM
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu