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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
KRT18 CYK18
Sequence Length (AA) Molecular Weight (Da)
430 48058
Protein Name
Keratin, type I cytoskeletal 18
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSFTTRSTFS TNYRSLGSVQ APSYGARPVS SAASVYAGAG GSGSRISVSR
60 70 80 90 100
STSFRGGMGS GGLATGIAGG LAGMGGIQNE KETMQSLNDR LASYLDRVRS
110 120 130 140 150
LETENRRLES KIREHLEKKG PQVRDWSHYF KIIEDLRAQI FANTVDNARI
160 170 180 190 200
VLQIDNARLA ADDFRVKYET ELAMRQSVEN DIHGLRKVID DTNITRLQLE
210 220 230 240 250
TEIEALKEEL LFMKKNHEEE VKGLQAQIAS SGLTVEVDAP KSQDLAKIMA
260 270 280 290 300
DIRAQYDELA RKNREELDKY WSQQIEESTT VVTTQSAEVG AAETTLTELR
310 320 330 340 350
RTVQSLEIDL DSMRNLKASL ENSLREVEAR YALQMEQLNG ILLHLESELA
360 370 380 390 400
QTRAEGQRQA QEYEALLNIK VKLEAEIATY RRLLEDGEDF NLGDALDSSN
410 420 430
SMQTIQKTTT RRIVDGKVVS ETNDTKVLRH

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1519 Collapse assay details

Data source: Panorama

Official Gene Symbol
KRT18
Peptide Sequence
AQIFANTVDNAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
138
Peptide End
149
CPTAC ID
CPTAC-1519
Peptide Molecular Mass
1,318.6630
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.4 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM1


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 6.8 7.2 5.6 37.7 30.9 28.4 38.3 31.7 28.9 15 15 15
y8 (1+) 7.5 5.2 2.1 37 29.4 27.7 37.8 29.9 27.8 15 15 15
y9 (1+) 5.3 7.4 2.2 37.8 29.6 28.2 38.2 30.5 28.3 15 15 15
sum 4.5 6.2 2.2 37.2 29.7 27.9 37.5 30.3 28 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-1518 Collapse assay details

Data source: Panorama

Official Gene Symbol
KRT18
Peptide Sequence
SLGSVQAPSYGAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
15
Peptide End
27
CPTAC ID
CPTAC-1518
Peptide Molecular Mass
1,291.6521
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.4 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM1


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 7.5 6.9 2.5 45 29.8 29.1 45.6 30.6 29.2 15 15 15
y8 (1+) 3.2 6.6 2.8 44.2 29.9 29.3 44.3 30.6 29.4 15 15 15
y9 (1+) 11.4 8.5 3.9 44.8 31.2 30.4 46.2 32.3 30.6 15 15 15
sum 5.1 6.1 2.2 44.3 29.9 29.2 44.6 30.5 29.3 15 15 15


Additional Resources and Comments