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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
PTMS N/A
Sequence Length (AA) Molecular Weight (Da)
102 11530
Protein Name
Parathymosin
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSEKSVEAAA ELSAKDLKEK KEKVEEKASR KERKKEVVEE EENGAEEEEE
60 70 80 90 100
ETAEDGEEED EGEEEDEEEE EEDDEGPALK RAAEEEDEAD PKRQKTENGA
102
SA

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1533 Collapse assay details

Data source: Panorama

Official Gene Symbol
PTMS
Peptide Sequence
AAEEEDEADPKR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
82
Peptide End
93
CPTAC ID
CPTAC-1533
Peptide Molecular Mass
1,358.5950
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.4 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM1


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (2+) 16.4 7.8 12.4 38.9 14.3 14.3 42.2 16.3 18.9 15 15 15
y9 (2+) 9.6 15.1 10.1 32.1 27.9 12.5 33.5 31.7 16.1 15 15 15
y10 (2+) 7.1 6.9 4.3 26.1 18.3 13.7 27 19.6 14.4 15 15 15
sum 6.9 6 3.9 27.8 18 12.3 28.6 19 12.9 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-1532 Collapse assay details

Data source: Panorama

Official Gene Symbol
PTMS
Peptide Sequence
SVEAAAELSAK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
5
Peptide End
15
CPTAC ID
CPTAC-1532
Peptide Molecular Mass
1,074.5557
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.4 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM1


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 18.1 4.4 6.8 54 37.8 40.1 57 38.1 40.7 15 15 15
y8 (1+) 14.7 8.8 3 53.4 36.3 37.5 55.4 37.4 37.6 15 15 15
y9 (1+) 4.8 4 3 63 36.6 37.4 63.2 36.8 37.5 15 15 15
sum 4.5 3.1 3 57.6 36.5 37.8 57.8 36.6 37.9 15 15 15


Additional Resources and Comments