OLFM4, CPTAC-1529 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
OLFM4	GW112

Sequence Length (AA)	Molecular Weight (Da)
510	57280

Protein Name
Olfactomedin-4

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MRPGLSFLLA	LLFFLGQAAG	DLGDVGPPIP	SPGFSSFPGV	DSSSSFSSSS
60	70	80	90	100
RSGSSSSRSL	GSGGSVSQLF	SNFTGSVDDR	GTCQCSVSLP	DTTFPVDRVE
110	120	130	140	150
RLEFTAHVLS	QKFEKELSKV	REYVQLISVY	EKKLLNLTVR	IDIMEKDTIS
160	170	180	190	200
YTELDFELIK	VEVKEMEKLV	IQLKESFGGS	SEIVDQLEVE	IRNMTLLVEK
210	220	230	240	250
LETLDKNNVL	AIRREIVALK	TKLKECEASK	DQNTPVVHPP	PTPGSCGHGG
260	270	280	290	300
VVNISKPSVV	QLNWRGFSYL	YGAWGRDYSP	QHPNKGLYWV	APLNTDGRLL
310	320	330	340	350
EYYRLYNTLD	DLLLYINARE	LRITYGQGSG	TAVYNNNMYV	NMYNTGNIAR
360	370	380	390	400
VNLTTNTIAV	TQTLPNAAYN	NRFSYANVAW	QDIDFAVDEN	GLWVIYSTEA
410	420	430	440	450
STGNMVISKL	NDTTLQVLNT	WYTKQYKPSA	SNAFMVCGVL	YATRTMNTRT
460	470	480	490	500
EEIFYYYDTN	TGKEGKLDIV	MHKMQEKVQS	INYNPFDQKL	YVYNDGYLLN
510
YDLSVLQKPQ

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1529 Collapse assay details

Data source: Panorama

Official Gene Symbol: OLFM4
Peptide Sequence: LYVYNDGYLLNYDLSVLQKPQ
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 490
Peptide End: 510

CPTAC ID: CPTAC-1529
Peptide Molecular Mass: 2,517.2791
Species: Homo sapiens (Human)
Assay Type: Direct MRM or SRM
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N P

LC: Waters nanoACQUITY (Part Number 176016000)
Column Packing: Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions: 100 um x 100 mm
Flow Rate: 0.4 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM1

CPTAC-1516: KRT17. ASLEGNLAETENR	CPTAC-1517: KRT17. TIVEEVQDGK	CPTAC-1518: KRT18. SLGSVQAPSYGAR
CPTAC-1519: KRT18. AQIFANTVDNAR	CPTAC-1520: KRT7. SAYGGPVGAGIR	CPTAC-1521: KRT7. SLDLDGIIAEVK
CPTAC-1522: KRT19. ALEAANGELEVK	CPTAC-1523: KRT19. AALEDTLAETEAR	CPTAC-1524: KRT8. YEELQSLAGK
CPTAC-1525: KRT8. LVSESSDVLPK	CPTAC-1526: OVGP1. ILYPEFNK	CPTAC-1527: OVGP1. THDFDGLDLFFLYPGLR
CPTAC-1528: OLFM4. VQSINYNPFDQK	CPTAC-1529: OLFM4. LYVYNDGYLLNYDLSVLQKPQ	CPTAC-1530: GNAI2. LLLLGAGESGK
CPTAC-1531: GNAI2. IAQSDYIPTQQDVLR	CPTAC-1532: PTMS. SVEAAAELSAK	CPTAC-1533: PTMS. AAEEEDEADPKR
CPTAC-1534: AP1S2. WYVPLSDK	CPTAC-1535: AP1S2. ELVQTVLAR	CPTAC-1536: ME3. VLTSDVEK
CPTAC-1537: ME3. GIFASGSPFK

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y3 (1+)	22.6	20.2	31.6	51.2	50.4	57	56	54.3	65.2	15	15	15
y4 (1+)	18.4	42.5	40.5	46	65.2	61.4	49.5	77.8	73.6	15	15	15
y5 (1+)	15.5	41.6	39.9	62.6	58.1	64.6	64.5	71.5	75.9	15	15	15
sum	14.5	19.2	33.3	51.8	52.3	57.7	53.8	55.7	66.6	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-1528 Collapse assay details

Data source: Panorama

Official Gene Symbol: OLFM4
Peptide Sequence: VQSINYNPFDQK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 478
Peptide End: 489

CPTAC ID: CPTAC-1528
Peptide Molecular Mass: 1,451.7045
Species: Homo sapiens (Human)
Assay Type: Direct MRM or SRM
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu