KRT18, CPTAC-1519 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
KRT18	CYK18

Sequence Length (AA)	Molecular Weight (Da)
430	48058

Protein Name
Keratin, type I cytoskeletal 18

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSFTTRSTFS	TNYRSLGSVQ	APSYGARPVS	SAASVYAGAG	GSGSRISVSR
60	70	80	90	100
STSFRGGMGS	GGLATGIAGG	LAGMGGIQNE	KETMQSLNDR	LASYLDRVRS
110	120	130	140	150
LETENRRLES	KIREHLEKKG	PQVRDWSHYF	KIIEDLRAQI	FANTVDNARI
160	170	180	190	200
VLQIDNARLA	ADDFRVKYET	ELAMRQSVEN	DIHGLRKVID	DTNITRLQLE
210	220	230	240	250
TEIEALKEEL	LFMKKNHEEE	VKGLQAQIAS	SGLTVEVDAP	KSQDLAKIMA
260	270	280	290	300
DIRAQYDELA	RKNREELDKY	WSQQIEESTT	VVTTQSAEVG	AAETTLTELR
310	320	330	340	350
RTVQSLEIDL	DSMRNLKASL	ENSLREVEAR	YALQMEQLNG	ILLHLESELA
360	370	380	390	400
QTRAEGQRQA	QEYEALLNIK	VKLEAEIATY	RRLLEDGEDF	NLGDALDSSN
410	420	430
SMQTIQKTTT	RRIVDGKVVS	ETNDTKVLRH

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for CPTAC-1519 Collapse assay details

Data source: Panorama

Official Gene Symbol: KRT18
Peptide Sequence: AQIFANTVDNAR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 138
Peptide End: 149

CPTAC ID: CPTAC-1519
Peptide Molecular Mass: 1,318.6630
Species: Homo sapiens (Human)
Assay Type: Direct MRM or SRM
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Waters nanoACQUITY (Part Number 176016000)
Column Packing: Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions: 100 um x 100 mm
Flow Rate: 0.4 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM1

CPTAC-1516: KRT17. ASLEGNLAETENR	CPTAC-1517: KRT17. TIVEEVQDGK	CPTAC-1518: KRT18. SLGSVQAPSYGAR
CPTAC-1519: KRT18. AQIFANTVDNAR	CPTAC-1520: KRT7. SAYGGPVGAGIR	CPTAC-1521: KRT7. SLDLDGIIAEVK
CPTAC-1522: KRT19. ALEAANGELEVK	CPTAC-1523: KRT19. AALEDTLAETEAR	CPTAC-1524: KRT8. YEELQSLAGK
CPTAC-1525: KRT8. LVSESSDVLPK	CPTAC-1526: OVGP1. ILYPEFNK	CPTAC-1527: OVGP1. THDFDGLDLFFLYPGLR
CPTAC-1528: OLFM4. VQSINYNPFDQK	CPTAC-1529: OLFM4. LYVYNDGYLLNYDLSVLQKPQ	CPTAC-1530: GNAI2. LLLLGAGESGK
CPTAC-1531: GNAI2. IAQSDYIPTQQDVLR	CPTAC-1532: PTMS. SVEAAAELSAK	CPTAC-1533: PTMS. AAEEEDEADPKR
CPTAC-1534: AP1S2. WYVPLSDK	CPTAC-1535: AP1S2. ELVQTVLAR	CPTAC-1536: ME3. VLTSDVEK
CPTAC-1537: ME3. GIFASGSPFK

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	6.8	7.2	5.6	37.7	30.9	28.4	38.3	31.7	28.9	15	15	15
y8 (1+)	7.5	5.2	2.1	37	29.4	27.7	37.8	29.9	27.8	15	15	15
y9 (1+)	5.3	7.4	2.2	37.8	29.6	28.2	38.2	30.5	28.3	15	15	15
sum	4.5	6.2	2.2	37.2	29.7	27.9	37.5	30.3	28	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-1518 Collapse assay details

Data source: Panorama

Official Gene Symbol: KRT18
Peptide Sequence: SLGSVQAPSYGAR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 15
Peptide End: 27

CPTAC ID: CPTAC-1518
Peptide Molecular Mass: 1,291.6521
Species: Homo sapiens (Human)
Assay Type: Direct MRM or SRM
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu