PIK3R2, CPTAC-1538 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
PIK3R2	N/A

Sequence Length (AA)	Molecular Weight (Da)
728	81545

Protein Name
Phosphatidylinositol 3-kinase regulatory subunit beta

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAGPEGFQYR	ALYPFRRERP	EDLELLPGDV	LVVSRAALQA	LGVAEGGERC
60	70	80	90	100
PQSVGWMPGL	NERTRQRGDF	PGTYVEFLGP	VALARPGPRP	RGPRPLPARP
110	120	130	140	150
RDGAPEPGLT	LPDLPEQFSP	PDVAPPLLVK	LVEAIERTGL	DSESHYRPEL
160	170	180	190	200
PAPRTDWSLS	DVDQWDTAAL	ADGIKSFLLA	LPAPLVTPEA	SAEARRALRE
210	220	230	240	250
AAGPVGPALE	PPTLPLHRAL	TLRFLLQHLG	RVASRAPALG	PAVRALGATF
260	270	280	290	300
GPLLLRAPPP	PSSPPPGGAP	DGSEPSPDFP	ALLVEKLLQE	HLEEQEVAPP
310	320	330	340	350
ALPPKPPKAK	PASTVLANGG	SPPSLQDAEW	YWGDISREEV	NEKLRDTPDG
360	370	380	390	400
TFLVRDASSK	IQGEYTLTLR	KGGNNKLIKV	FHRDGHYGFS	EPLTFCSVVD
410	420	430	440	450
LINHYRHESL	AQYNAKLDTR	LLYPVSKYQQ	DQIVKEDSVE	AVGAQLKVYH
460	470	480	490	500
QQYQDKSREY	DQLYEEYTRT	SQELQMKRTA	IEAFNETIKI	FEEQGQTQEK
510	520	530	540	550
CSKEYLERFR	REGNEKEMQR	ILLNSERLKS	RIAEIHESRT	KLEQQLRAQA
560	570	580	590	600
SDNREIDKRM	NSLKPDLMQL	RKIRDQYLVW	LTQKGARQKK	INEWLGIKNE
610	620	630	640	650
TEDQYALMED	EDDLPHHEER	TWYVGKINRT	QAEEMLSGKR	DGTFLIRESS
660	670	680	690	700
QRGCYACSVV	VDGDTKHCVI	YRTATGFGFA	EPYNLYGSLK	ELVLHYQHAS
710	720	728
LVQHNDALTV	TLAHPVRAPG	PGPPPAAR

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1538 Collapse assay details

Data source: Panorama

Official Gene Symbol: PIK3R2
Peptide Sequence: ALGATFGPLLLR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 245
Peptide End: 256

CPTAC ID: CPTAC-1538
Peptide Molecular Mass: 1,227.7339
Species: Homo sapiens (Human)
Assay Type: Direct MRM or SRM
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Waters nanoACQUITY (Part Number 176016000)
Column Packing: Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions: 100 um x 100 mm
Flow Rate: 0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM2

CPTAC-1538: PIK3R2. ALGATFGPLLLR	CPTAC-1539: PIK3R1. FSAASSDNTENLIK	CPTAC-1540: PDPK1. QLLLTEGPHLYYVDPVNK
CPTAC-1541: MAPK1. VADPDHDHTGFLTEYVATR	CPTAC-1542: MAPK3. IADPEHDHTGFLTEYVATR	CPTAC-1543: MAP2K2. ISELGAGNGGVVTK
CPTAC-1544: MAP2K1. ISELGAGNGGVVFK	CPTAC-1545: MAP2K1. LIHLEIKPAIR	CPTAC-1546: RAF1. TISNGFGFK
CPTAC-1547: ARAF. TQHC[+57.0]DPEHFPFPAPANAPLQR	CPTAC-1548: RASA1. LLAITELLQQK	CPTAC-1549: NRAS. SFADINLYR
CPTAC-1550: KRAS. SFEDIHHYR	CPTAC-1551: HRAS. LVVVGAGGVGK	CPTAC-1552: HRAS. SFEDIHQYR
CPTAC-1553: SOS2. LPGYSSAEYR	CPTAC-1554: SOS1. LPSADVYR	CPTAC-1555: SOS1. EYIQPVQLR
CPTAC-1556: PTPN11. SNPGDFTLSVR	CPTAC-1557: GAB1. LTGDPDVLEYYK	CPTAC-1558: GRB2. ATADDELSFK
CPTAC-1559: SHC1. EAISLVC[+57.0]EAVPGAK	CPTAC-1560: EGFR. LTQLGTFEDHFLSLQR	CPTAC-1561: ADAM17. NIYLNSGLTSTK
CPTAC-1562: SHOC2. LDSLTTLYLR	CPTAC-1563: SPRED1. VPHQEENGC[+57.0]ADFFIR	CPTAC-1564: DUSP6. DSTNLDVLEEFGIK
CPTAC-1565: DUSP4. GSVSLEQILPAEEEVR	CPTAC-1566: ERRFI1. NSPSLFPC[+57.0]APLC[+57.0]ER	CPTAC-1567: CBL. GTEPIVVDPFDPR
CPTAC-1568: PEBP1. VLTPTQVK	CPTAC-1569: PTPRE. TGTFIALSNILER	CPTAC-1570: SPRY4. LQHPLTILPIDQVK

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8 (1+)	12.5	4.2	3.7	19.7	4.9	5.4	23.3	6.5	6.5	15	15	15
y6 (1+)	5.7	3.9	4.5	14.7	4.2	6.9	15.8	5.7	8.2	15	15	15
y5 (1+)	15.5	4.2	4.7	19.7	5.2	5.9	25.1	6.7	7.5	15	15	15
sum	5	3.3	2.9	15.2	4	5.6	16	5.2	6.3	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for CPTAC-2802 Collapse assay details

Data source: Panorama

Official Gene Symbol: PIK3R2
Peptide Modified Sequence: GC[+57.0]YAC[+57.0]SVVVDGDTK
Modification Type: Carbamidomethyl (C), Carbamidomethyl (C)
Protein - Site of Modification: 654, 657
Peptide - Site of Modification: 2, 5
Peptide Start: 653
Peptide End: 666

CPTAC ID: CPTAC-2802
Peptide Molecular Mass: 1,529.6490
Species: Homo Sapiens
Assay Type: Direct MRM or SRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend