PIK3R1, CPTAC-1539 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
PIK3R1	GRB1

Sequence Length (AA)	Molecular Weight (Da)
724	83598

Protein Name
Phosphatidylinositol 3-kinase regulatory subunit alpha

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSAEGYQYRA	LYDYKKEREE	DIDLHLGDIL	TVNKGSLVAL	GFSDGQEARP
60	70	80	90	100
EEIGWLNGYN	ETTGERGDFP	GTYVEYIGRK	KISPPTPKPR	PPRPLPVAPG
110	120	130	140	150
SSKTEADVEQ	QALTLPDLAE	QFAPPDIAPP	LLIKLVEAIE	KKGLECSTLY
160	170	180	190	200
RTQSSSNLAE	LRQLLDCDTP	SVDLEMIDVH	VLADAFKRYL	LDLPNPVIPA
210	220	230	240	250
AVYSEMISLA	PEVQSSEEYI	QLLKKLIRSP	SIPHQYWLTL	QYLLKHFFKL
260	270	280	290	300
SQTSSKNLLN	ARVLSEIFSP	MLFRFSAASS	DNTENLIKVI	EILISTEWNE
310	320	330	340	350
RQPAPALPPK	PPKPTTVANN	GMNNNMSLQD	AEWYWGDISR	EEVNEKLRDT
360	370	380	390	400
ADGTFLVRDA	STKMHGDYTL	TLRKGGNNKL	IKIFHRDGKY	GFSDPLTFSS
410	420	430	440	450
VVELINHYRN	ESLAQYNPKL	DVKLLYPVSK	YQQDQVVKED	NIEAVGKKLH
460	470	480	490	500
EYNTQFQEKS	REYDRLYEEY	TRTSQEIQMK	RTAIEAFNET	IKIFEEQCQT
510	520	530	540	550
QERYSKEYIE	KFKREGNEKE	IQRIMHNYDK	LKSRISEIID	SRRRLEEDLK
560	570	580	590	600
KQAAEYREID	KRMNSIKPDL	IQLRKTRDQY	LMWLTQKGVR	QKKLNEWLGN
610	620	630	640	650
ENTEDQYSLV	EDDEDLPHHD	EKTWNVGSSN	RNKAENLLRG	KRDGTFLVRE
660	670	680	690	700
SSKQGCYACS	VVVDGEVKHC	VINKTATGYG	FAEPYNLYSS	LKELVLHYQH
710	720	724
TSLVQHNDSL	NVTLAYPVYA	QQRR

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1539 Collapse assay details

Data source: Panorama

Official Gene Symbol: PIK3R1
Peptide Sequence: FSAASSDNTENLIK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 275
Peptide End: 288

CPTAC ID: CPTAC-1539
Peptide Molecular Mass: 1,495.7155
Species: Homo sapiens (Human)
Assay Type: Direct MRM or SRM
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Waters nanoACQUITY (Part Number 176016000)
Column Packing: Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions: 100 um x 100 mm
Flow Rate: 0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM2

CPTAC-1538: PIK3R2. ALGATFGPLLLR	CPTAC-1539: PIK3R1. FSAASSDNTENLIK	CPTAC-1540: PDPK1. QLLLTEGPHLYYVDPVNK
CPTAC-1541: MAPK1. VADPDHDHTGFLTEYVATR	CPTAC-1542: MAPK3. IADPEHDHTGFLTEYVATR	CPTAC-1543: MAP2K2. ISELGAGNGGVVTK
CPTAC-1544: MAP2K1. ISELGAGNGGVVFK	CPTAC-1545: MAP2K1. LIHLEIKPAIR	CPTAC-1546: RAF1. TISNGFGFK
CPTAC-1547: ARAF. TQHC[+57.0]DPEHFPFPAPANAPLQR	CPTAC-1548: RASA1. LLAITELLQQK	CPTAC-1549: NRAS. SFADINLYR
CPTAC-1550: KRAS. SFEDIHHYR	CPTAC-1551: HRAS. LVVVGAGGVGK	CPTAC-1552: HRAS. SFEDIHQYR
CPTAC-1553: SOS2. LPGYSSAEYR	CPTAC-1554: SOS1. LPSADVYR	CPTAC-1555: SOS1. EYIQPVQLR
CPTAC-1556: PTPN11. SNPGDFTLSVR	CPTAC-1557: GAB1. LTGDPDVLEYYK	CPTAC-1558: GRB2. ATADDELSFK
CPTAC-1559: SHC1. EAISLVC[+57.0]EAVPGAK	CPTAC-1560: EGFR. LTQLGTFEDHFLSLQR	CPTAC-1561: ADAM17. NIYLNSGLTSTK
CPTAC-1562: SHOC2. LDSLTTLYLR	CPTAC-1563: SPRED1. VPHQEENGC[+57.0]ADFFIR	CPTAC-1564: DUSP6. DSTNLDVLEEFGIK
CPTAC-1565: DUSP4. GSVSLEQILPAEEEVR	CPTAC-1566: ERRFI1. NSPSLFPC[+57.0]APLC[+57.0]ER	CPTAC-1567: CBL. GTEPIVVDPFDPR
CPTAC-1568: PEBP1. VLTPTQVK	CPTAC-1569: PTPRE. TGTFIALSNILER	CPTAC-1570: SPRY4. LQHPLTILPIDQVK

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y11 (1+)	11.9	6	5.6	12.1	6.6	8.8	17	8.9	10.4	15	15	15
y10 (1+)	8.8	5.6	2.9	9.2	5.5	7	12.7	7.8	7.6	15	15	15
y9 (1+)	9.9	5.4	8.1	10.2	5.9	8.2	14.2	8	11.5	15	15	15
y8 (1+)	9.3	8.1	6.3	18.5	17	13.2	20.7	18.8	14.6	15	15	15
sum	6.1	4.2	3	7.4	5	6.9	9.6	6.5	7.5	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for CPTAC-1740 Collapse assay details

Data source: Panorama

Official Gene Symbol: PIK3R1
Peptide Modified Sequence: LY[+80.0]EEYTR
Modification Type: Phospho (Y)
Protein - Site of Modification: 467
Peptide - Site of Modification: 2
Peptide Start: 466
Peptide End: 472

CPTAC ID: CPTAC-1740
Peptide Molecular Mass: 1,052.4216
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM or SRM
Enrichment Method: N/A
Matrix: Ovarian cancer cell lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu