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Overview Data source: UniProt

Official Gene Symbol Other Aliases
SPRY4 N/A
Sequence Length (AA) Molecular Weight (Da)
299 32541
Protein Name
Protein sprouty homolog 4
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MEPPIPQSAP LTPNSVMVQP LLDSRMSHSR LQHPLTILPI DQVKTSHVEN
60 70 80 90 100
DYIDNPSLAL TTGPKRTRGG APELAPTPAR CDQDVTHHWI SFSGRPSSVS
110 120 130 140 150
SSSSTSSDQR LLDHMAPPPV ADQASPRAVR IQPKVVHCQP LDLKGPAVPP
160 170 180 190 200
ELDKHFLLCE ACGKCKCKEC ASPRTLPSCW VCNQECLCSA QTLVNYGTCM
210 220 230 240 250
CLVQGIFYHC TNEDDEGSCA DHPCSCSRSN CCARWSFMGA LSVVLPCLLC
260 270 280 290 299
YLPATGCVKL AQRGYDRLRR PGCRCKHTNS VICKAASGDA KTSRPDKPF

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1570 Collapse assay details

Data source: Panorama

Official Gene Symbol
SPRY4
Peptide Sequence
LQHPLTILPIDQVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
31
Peptide End
44
CPTAC ID
CPTAC-1570
Peptide Molecular Mass
1,613.9505
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM2


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 4.4 4.1 5.5 11.4 4.5 9.2 12.2 6.1 10.7 15 15 15
y5 (1+) 19.9 8.6 6.4 21.8 11.6 13 29.5 14.4 14.5 15 15 15
y4 (1+) 19.6 7.1 8.1 18.9 6.3 11.7 27.2 9.5 14.2 15 15 15
b7 (1+) 7.6 3.7 1.9 15.5 3.5 6.3 17.3 5.1 6.6 15 15 15
sum 3.2 3.4 3.9 11.2 3.9 8.3 11.6 5.2 9.2 15 15 15

Additional Resources and Comments