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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
DUSP4 MKP2, VH2
Sequence Length (AA) Molecular Weight (Da)
394 42953
Protein Name
Dual specificity protein phosphatase 4
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MVTMEELREM DCSVLKRLMN RDENGGGAGG SGSHGTLGLP SGGKCLLLDC
60 70 80 90 100
RPFLAHSAGY ILGSVNVRCN TIVRRRAKGS VSLEQILPAE EEVRARLRSG
110 120 130 140 150
LYSAVIVYDE RSPRAESLRE DSTVSLVVQA LRRNAERTDI CLLKGGYERF
160 170 180 190 200
SSEYPEFCSK TKALAAIPPP VPPSATEPLD LGCSSCGTPL HDQGGPVEIL
210 220 230 240 250
PFLYLGSAYH AARRDMLDAL GITALLNVSS DCPNHFEGHY QYKCIPVEDN
260 270 280 290 300
HKADISSWFM EAIEYIDAVK DCRGRVLVHC QAGISRSATI CLAYLMMKKR
310 320 330 340 350
VRLEEAFEFV KQRRSIISPN FSFMGQLLQF ESQVLATSCA AEAASPSGPL
360 370 380 390 394
RERGKTPATP TSQFVFSFPV SVGVHSAPSS LPYLHSPITT SPSC

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1565 Collapse assay details

Data source: Panorama

Official Gene Symbol
DUSP4
Peptide Sequence
GSVSLEQILPAEEEVR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
79
Peptide End
94
CPTAC ID
CPTAC-1565
Peptide Molecular Mass
1,754.9050
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM2


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 12.2 4.5 2.9 21.2 5.5 6.5 24.5 7.1 7.1 15 15 15
y8 (1+) 9.8 3.8 1.3 15.3 4.6 6.7 18.2 6 6.8 15 15 15
y7 (1+) 10.3 3.4 1.9 16.8 6 7.4 19.7 6.9 7.6 15 15 15
b8 (1+) 21.2 3.6 3.9 19.7 5.6 7.4 28.9 6.7 8.4 15 15 15
sum 6.6 2.6 1.3 14 5.2 6.9 15.5 5.8 7 15 15 15


Additional Resources and Comments