PTPN11, CPTAC-1556 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
PTPN11	PTP2C, SHPTP2

Sequence Length (AA)	Molecular Weight (Da)
597	68436

Protein Name
Tyrosine-protein phosphatase non-receptor type 11

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MTSRRWFHPN	ITGVEAENLL	LTRGVDGSFL	ARPSKSNPGD	FTLSVRRNGA
60	70	80	90	100
VTHIKIQNTG	DYYDLYGGEK	FATLAELVQY	YMEHHGQLKE	KNGDVIELKY
110	120	130	140	150
PLNCADPTSE	RWFHGHLSGK	EAEKLLTEKG	KHGSFLVRES	QSHPGDFVLS
160	170	180	190	200
VRTGDDKGES	NDGKSKVTHV	MIRCQELKYD	VGGGERFDSL	TDLVEHYKKN
210	220	230	240	250
PMVETLGTVL	QLKQPLNTTR	INAAEIESRV	RELSKLAETT	DKVKQGFWEE
260	270	280	290	300
FETLQQQECK	LLYSRKEGQR	QENKNKNRYK	NILPFDHTRV	VLHDGDPNEP
310	320	330	340	350
VSDYINANII	MPEFETKCNN	SKPKKSYIAT	QGCLQNTVND	FWRMVFQENS
360	370	380	390	400
RVIVMTTKEV	ERGKSKCVKY	WPDEYALKEY	GVMRVRNVKE	SAAHDYTLRE
410	420	430	440	450
LKLSKVGQAL	LQGNTERTVW	QYHFRTWPDH	GVPSDPGGVL	DFLEEVHHKQ
460	470	480	490	500
ESIMDAGPVV	VHCSAGIGRT	GTFIVIDILI	DIIREKGVDC	DIDVPKTIQM
510	520	530	540	550
VRSQRSGMVQ	TEAQYRFIYM	AVQHYIETLQ	RRIEEEQKSK	RKGHEYTNIK
560	570	580	590	597
YSLADQTSGD	QSPLPPCTPT	PPCAEMREDS	ARVYENVGLM	QQQKSFR

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for CPTAC-1556 Collapse assay details

Data source: Panorama

Official Gene Symbol: PTPN11
Peptide Sequence: SNPGDFTLSVR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 36
Peptide End: 46

CPTAC ID: CPTAC-1556
Peptide Molecular Mass: 1,191.5884
Species: Homo sapiens (Human)
Assay Type: Direct MRM or SRM
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Waters nanoACQUITY (Part Number 176016000)
Column Packing: Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions: 100 um x 100 mm
Flow Rate: 0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM2

CPTAC-1538: PIK3R2. ALGATFGPLLLR	CPTAC-1539: PIK3R1. FSAASSDNTENLIK	CPTAC-1540: PDPK1. QLLLTEGPHLYYVDPVNK
CPTAC-1541: MAPK1. VADPDHDHTGFLTEYVATR	CPTAC-1542: MAPK3. IADPEHDHTGFLTEYVATR	CPTAC-1543: MAP2K2. ISELGAGNGGVVTK
CPTAC-1544: MAP2K1. ISELGAGNGGVVFK	CPTAC-1545: MAP2K1. LIHLEIKPAIR	CPTAC-1546: RAF1. TISNGFGFK
CPTAC-1547: ARAF. TQHC[+57.0]DPEHFPFPAPANAPLQR	CPTAC-1548: RASA1. LLAITELLQQK	CPTAC-1549: NRAS. SFADINLYR
CPTAC-1550: KRAS. SFEDIHHYR	CPTAC-1551: HRAS. LVVVGAGGVGK	CPTAC-1552: HRAS. SFEDIHQYR
CPTAC-1553: SOS2. LPGYSSAEYR	CPTAC-1554: SOS1. LPSADVYR	CPTAC-1555: SOS1. EYIQPVQLR
CPTAC-1556: PTPN11. SNPGDFTLSVR	CPTAC-1557: GAB1. LTGDPDVLEYYK	CPTAC-1558: GRB2. ATADDELSFK
CPTAC-1559: SHC1. EAISLVC[+57.0]EAVPGAK	CPTAC-1560: EGFR. LTQLGTFEDHFLSLQR	CPTAC-1561: ADAM17. NIYLNSGLTSTK
CPTAC-1562: SHOC2. LDSLTTLYLR	CPTAC-1563: SPRED1. VPHQEENGC[+57.0]ADFFIR	CPTAC-1564: DUSP6. DSTNLDVLEEFGIK
CPTAC-1565: DUSP4. GSVSLEQILPAEEEVR	CPTAC-1566: ERRFI1. NSPSLFPC[+57.0]APLC[+57.0]ER	CPTAC-1567: CBL. GTEPIVVDPFDPR
CPTAC-1568: PEBP1. VLTPTQVK	CPTAC-1569: PTPRE. TGTFIALSNILER	CPTAC-1570: SPRY4. LQHPLTILPIDQVK

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y9 (1+)	12.7	2.8	2.1	15.2	3.2	7.8	19.8	4.3	8.1	15	15	15
y8 (1+)	17.3	3.3	3.9	20.9	2.7	5.8	27.1	4.3	7	15	15	15
y9 (2+)	6.8	4.2	4	12.5	6	7.8	14.2	7.3	8.8	15	15	15
sum	5.8	3.7	3.3	11.4	5	7.3	12.8	6.2	8	15	15	15

PTPN11

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1556 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

Search form

PTPN11

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1556 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments