SHC1, CPTAC-1559 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
SHC1	SHC, SHCA

Sequence Length (AA)	Molecular Weight (Da)
583	62822

Protein Name
SHC-transforming protein 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MDLLPPKPKY	NPLRNESLSS	LEEGASGSTP	PEELPSPSAS	SLGPILPPLP
60	70	80	90	100
GDDSPTTLCS	FFPRMSNLRL	ANPAGGRPGS	KGEPGRAADD	GEGIVGAAMP
110	120	130	140	150
DSGPLPLLQD	MNKLSGGGGR	RTRVEGGQLG	GEEWTRHGSF	VNKPTRGWLH
160	170	180	190	200
PNDKVMGPGV	SYLVRYMGCV	EVLQSMRALD	FNTRTQVTRE	AISLVCEAVP
210	220	230	240	250
GAKGATRRRK	PCSRPLSSIL	GRSNLKFAGM	PITLTVSTSS	LNLMAADCKQ
260	270	280	290	300
IIANHHMQSI	SFASGGDPDT	AEYVAYVAKD	PVNQRACHIL	ECPEGLAQDV
310	320	330	340	350
ISTIGQAFEL	RFKQYLRNPP	KLVTPHDRMA	GFDGSAWDEE	EEEPPDHQYY
360	370	380	390	400
NDFPGKEPPL	GGVVDMRLRE	GAAPGAARPT	APNAQTPSHL	GATLPVGQPV
410	420	430	440	450
GGDPEVRKQM	PPPPPCPGRE	LFDDPSYVNV	QNLDKARQAV	GGAGPPNPAI
460	470	480	490	500
NGSAPRDLFD	MKPFEDALRV	PPPPQSVSMA	EQLRGEPWFH	GKLSRREAEA
510	520	530	540	550
LLQLNGDFLV	RESTTTPGQY	VLTGLQSGQP	KHLLLVDPEG	VVRTKDHRFE
560	570	580	583
SVSHLISYHM	DNHLPIISAG	SELCLQQPVE	RKL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-1366 Collapse assay details

Data source: Panorama

Official Gene Symbol: SHC1
Peptide Modified Sequence: ELFDDPSY[+80.0]VNVQNLDK
Modification Type: Phospho (Y)
Protein - Site of Modification: 427
Peptide - Site of Modification: 8
Peptide Start: 420
Peptide End: 435

CPTAC ID: CPTAC-1366
Peptide Molecular Mass: 1,974.8612
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM or SRM
Enrichment Method: N/A
Matrix: digested cell lysate
Submitting Laboratory: Broad Institute
Submitting Lab PI: Steven A. Carr

Publication

View Details (opens in a new window)

Mol Cell Proteomics. 2016 May;15(5):1622-41. doi: 10.1074/mcp.M116.058354. Epub 2016 Feb 24. Reduced-representation Phosphosignatures Measured by Quantitative Targeted MS Capture Cellular States and Enable Large-scale Comparison of Drug-induced Phenotypes. Abelin JG1, Patel J1, Lu X1, Feeney CM1, Fagbami L1, Creech AL1, Hu R1, Lam D1, Davison D1, Pino L1, Qiao JW1, Kuhn E1, Officer A1, Li J2, Abbatiello S1, Subramanian A1, Sidman R2, Snyder E3, Carr SA1, Jaffe JD4.

View Details (opens in a new window)

Mol Cell Proteomics. 2014 Jul;13(7):1690-704. doi: 10.1074/mcp.M113.036392. Epub 2014 Apr 9. Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mertins P1, Yang F2, Liu T2, Mani DR3, Petyuk VA2, Gillette MA3, Clauser KR3, Qiao JW3, Gritsenko MA2, Moore RJ2, Levine DA4, Townsend R5, Erdmann-Gilmore P5, Snider JE5, Davies SR5, Ruggles KV6, Fenyo D6, Kitchens RT5, Li S5, Olvera N4, Dao F4, Rodriguez H7, Chan DW8, Liebler D9, White F10, Rodland KD2, Mills GB11, Smith RD2, Paulovich AG12, Ellis M5, Carr SA1

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Quantiva
Internal Standard: light stable isotope peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy NanoLC1000
Column Packing: Reprosil C18, 1.9um, 200A
Column Dimensions: 0.075 x 150 mm
Flow Rate: 200 nL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM2

CPTAC-1538: PIK3R2. ALGATFGPLLLR	CPTAC-1539: PIK3R1. FSAASSDNTENLIK	CPTAC-1540: PDPK1. QLLLTEGPHLYYVDPVNK
CPTAC-1541: MAPK1. VADPDHDHTGFLTEYVATR	CPTAC-1542: MAPK3. IADPEHDHTGFLTEYVATR	CPTAC-1543: MAP2K2. ISELGAGNGGVVTK
CPTAC-1544: MAP2K1. ISELGAGNGGVVFK	CPTAC-1545: MAP2K1. LIHLEIKPAIR	CPTAC-1546: RAF1. TISNGFGFK
CPTAC-1547: ARAF. TQHC[+57.0]DPEHFPFPAPANAPLQR	CPTAC-1548: RASA1. LLAITELLQQK	CPTAC-1549: NRAS. SFADINLYR
CPTAC-1550: KRAS. SFEDIHHYR	CPTAC-1551: HRAS. LVVVGAGGVGK	CPTAC-1552: HRAS. SFEDIHQYR
CPTAC-1553: SOS2. LPGYSSAEYR	CPTAC-1554: SOS1. LPSADVYR	CPTAC-1555: SOS1. EYIQPVQLR
CPTAC-1556: PTPN11. SNPGDFTLSVR	CPTAC-1557: GAB1. LTGDPDVLEYYK	CPTAC-1558: GRB2. ATADDELSFK
CPTAC-1559: SHC1. EAISLVC[+57.0]EAVPGAK	CPTAC-1560: EGFR. LTQLGTFEDHFLSLQR	CPTAC-1561: ADAM17. NIYLNSGLTSTK
CPTAC-1562: SHOC2. LDSLTTLYLR	CPTAC-1563: SPRED1. VPHQEENGC[+57.0]ADFFIR	CPTAC-1564: DUSP6. DSTNLDVLEEFGIK
CPTAC-1565: DUSP4. GSVSLEQILPAEEEVR	CPTAC-1566: ERRFI1. NSPSLFPC[+57.0]APLC[+57.0]ER	CPTAC-1567: CBL. GTEPIVVDPFDPR
CPTAC-1568: PEBP1. VLTPTQVK	CPTAC-1569: PTPRE. TGTFIALSNILER	CPTAC-1570: SPRY4. LQHPLTILPIDQVK

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y11 (2+)	7.9	5.4	2.5	9.2	8.8	8.9	12.1	10.3	9.2	15	15	15
y5 (1+)	4.6	5.1	3.7	5.7	9.4	9.9	7.3	10.7	10.6	14	15	15
y7 (1+)	3.6	7	4.4	3.3	10.5	10.2	4.9	12.6	11.1	14	15	15
sum	6.6	5	2.2	8.8	9	9.1	11	10.3	9.4	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for CPTAC-1559 Collapse assay details

Data source: Panorama

Official Gene Symbol: SHC1
Peptide Modified Sequence: EAISLVC[+57.0]EAVPGAK
Modification Type: Carbamidomethyl (C)
Protein - Site of Modification: 196
Peptide - Site of Modification: 7
Peptide Start: 190
Peptide End: 203

CPTAC ID: CPTAC-1559
Peptide Molecular Mass: 1,442.7439
Species: Homo sapiens (Human)
Assay Type: Direct MRM or SRM
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu