ADAM17, CPTAC-1561 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
ADAM17	CSVP, TACE

Sequence Length (AA)	Molecular Weight (Da)
824	93021

Protein Name
Disintegrin and metalloproteinase domain-containing protein 17

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MRQSLLFLTS	VVPFVLAPRP	PDDPGFGPHQ	RLEKLDSLLS	DYDILSLSNI
60	70	80	90	100
QQHSVRKRDL	QTSTHVETLL	TFSALKRHFK	LYLTSSTERF	SQNFKVVVVD
110	120	130	140	150
GKNESEYTVK	WQDFFTGHVV	GEPDSRVLAH	IRDDDVIIRI	NTDGAEYNIE
160	170	180	190	200
PLWRFVNDTK	DKRMLVYKSE	DIKNVSRLQS	PKVCGYLKVD	NEELLPKGLV
210	220	230	240	250
DREPPEELVH	RVKRRADPDP	MKNTCKLLVV	ADHRFYRYMG	RGEESTTTNY
260	270	280	290	300
LIELIDRVDD	IYRNTSWDNA	GFKGYGIQIE	QIRILKSPQE	VKPGEKHYNM
310	320	330	340	350
AKSYPNEEKD	AWDVKMLLEQ	FSFDIAEEAS	KVCLAHLFTY	QDFDMGTLGL
360	370	380	390	400
AYVGSPRANS	HGGVCPKAYY	SPVGKKNIYL	NSGLTSTKNY	GKTILTKEAD
410	420	430	440	450
LVTTHELGHN	FGAEHDPDGL	AECAPNEDQG	GKYVMYPIAV	SGDHENNKMF
460	470	480	490	500
SNCSKQSIYK	TIESKAQECF	QERSNKVCGN	SRVDEGEECD	PGIMYLNNDT
510	520	530	540	550
CCNSDCTLKE	GVQCSDRNSP	CCKNCQFETA	QKKCQEAINA	TCKGVSYCTG
560	570	580	590	600
NSSECPPPGN	AEDDTVCLDL	GKCKDGKCIP	FCEREQQLES	CACNETDNSC
610	620	630	640	650
KVCCRDLSGR	CVPYVDAEQK	NLFLRKGKPC	TVGFCDMNGK	CEKRVQDVIE
660	670	680	690	700
RFWDFIDQLS	INTFGKFLAD	NIVGSVLVFS	LIFWIPFSIL	VHCVDKKLDK
710	720	730	740	750
QYESLSLFHP	SNVEMLSSMD	SASVRIIKPF	PAPQTPGRLQ	PAPVIPSAPA
760	770	780	790	800
APKLDHQRMD	TIQEDPSTDS	HMDEDGFEKD	PFPNSSTAAK	SFEDLTDHPV
810	820	824
TRSEKAASFK	LQRQNRVDSK	ETEC

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-5968 Collapse assay details

Data source: Panorama

Official Gene Symbol: ADAM17
Peptide Sequence: VDNEELLPK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 4
Peptide Start: 189
Peptide End: 197

CPTAC ID: CPTAC-5968
Peptide Molecular Mass: 1,055.5499
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM or SRM
Enrichment Method: N/A
Matrix: FFPE tumor tissue lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Targeted Mass Spectrometry Enables Multiplexed Quantification of Immunomodulatory Proteins in Clinical Biospecimens.Whiteaker JR, Lundeen RA, Zhao L, Schoenherr RM, Burian A, Huang D, Voytovich U, Wang T, Kennedy JJ, Ivey RG, Lin C, Murillo OD, Lorentzen TD, Thiagarajan M, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Reading JJ, Kaczmarczyk JA, Richardson CW, Garcia-Buntley SS, Bocik W, Hewitt SM, Murray KE, Do N, Brophy M, Wilz SW, Yu H, Ajjarapu S, Boja E, Hiltke T, Rodriguez H, Paulovich AG.Front Immunol. 2021 Nov 11;12:765898. doi: 10.3389/fimmu.2021.765898. eCollection 2021.PMID: 34858420

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTRAP 5500
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C at C-terminus K

LC: Eksigent nanoLC 425
Column Packing: 3 µm ChromXP C18EP, 120 Å
Column Dimensions: 150 x 0.3 mm
Flow Rate: 0.3 µL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM2

CPTAC-1538: PIK3R2. ALGATFGPLLLR	CPTAC-1539: PIK3R1. FSAASSDNTENLIK	CPTAC-1540: PDPK1. QLLLTEGPHLYYVDPVNK
CPTAC-1541: MAPK1. VADPDHDHTGFLTEYVATR	CPTAC-1542: MAPK3. IADPEHDHTGFLTEYVATR	CPTAC-1543: MAP2K2. ISELGAGNGGVVTK
CPTAC-1544: MAP2K1. ISELGAGNGGVVFK	CPTAC-1545: MAP2K1. LIHLEIKPAIR	CPTAC-1546: RAF1. TISNGFGFK
CPTAC-1547: ARAF. TQHC[+57.0]DPEHFPFPAPANAPLQR	CPTAC-1548: RASA1. LLAITELLQQK	CPTAC-1549: NRAS. SFADINLYR
CPTAC-1550: KRAS. SFEDIHHYR	CPTAC-1551: HRAS. LVVVGAGGVGK	CPTAC-1552: HRAS. SFEDIHQYR
CPTAC-1553: SOS2. LPGYSSAEYR	CPTAC-1554: SOS1. LPSADVYR	CPTAC-1555: SOS1. EYIQPVQLR
CPTAC-1556: PTPN11. SNPGDFTLSVR	CPTAC-1557: GAB1. LTGDPDVLEYYK	CPTAC-1558: GRB2. ATADDELSFK
CPTAC-1559: SHC1. EAISLVC[+57.0]EAVPGAK	CPTAC-1560: EGFR. LTQLGTFEDHFLSLQR	CPTAC-1561: ADAM17. NIYLNSGLTSTK
CPTAC-1562: SHOC2. LDSLTTLYLR	CPTAC-1563: SPRED1. VPHQEENGC[+57.0]ADFFIR	CPTAC-1564: DUSP6. DSTNLDVLEEFGIK
CPTAC-1565: DUSP4. GSVSLEQILPAEEEVR	CPTAC-1566: ERRFI1. NSPSLFPC[+57.0]APLC[+57.0]ER	CPTAC-1567: CBL. GTEPIVVDPFDPR
CPTAC-1568: PEBP1. VLTPTQVK	CPTAC-1569: PTPRE. TGTFIALSNILER	CPTAC-1570: SPRY4. LQHPLTILPIDQVK

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	43.3	19	5.9	36.5	24.5	20.3	56.6	31	21.1	13	25	14
y5 (1+)	42.2	20.5	6.6	41.5	23	18.9	59.2	30.8	20	13	25	14
y8 (1+)	18.4	13.2	6.4	15.3	17	19.1	23.9	21.5	20.1	13	25	14
sum	20.6	9.7	4.8	21.7	15.8	19	29.9	18.5	19.6	13	25	14

Additional Resources and Comments

Resources

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View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-1561 Collapse assay details

Data source: Panorama

Official Gene Symbol: ADAM17
Peptide Sequence: NIYLNSGLTSTK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 377
Peptide End: 388

CPTAC ID: CPTAC-1561
Peptide Molecular Mass: 1,309.6878
Species: Homo sapiens (Human)
Assay Type: Direct MRM or SRM
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu