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Overview Data source: UniProt

Official Gene Symbol Other Aliases
HRAS HRAS1
Sequence Length (AA) Molecular Weight (Da)
189 21298
Protein Name
GTPase HRas
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET
60 70 80 90 100
CLLDILDTAG QEEYSAMRDQ YMRTGEGFLC VFAINNTKSF EDIHQYREQI
110 120 130 140 150
KRVKDSDDVP MVLVGNKCDL AARTVESRQA QDLARSYGIP YIETSAKTRQ
160 170 180 189
GVEDAFYTLV REIRQHKLRK LNPPDESGPG CMSCKCVLS

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1551 Collapse assay details

Data source: Panorama

Official Gene Symbol
HRAS
Peptide Sequence
LVVVGAGGVGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
6
Peptide End
16
CPTAC ID
CPTAC-1551
Peptide Molecular Mass
954.5862
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM2


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 6.5 3.3 2.4 9.5 4.2 7.5 11.5 5.3 7.9 15 15 15
y8 (1+) 9.5 3.3 2.7 9.3 5 6.5 13.3 6 7 15 15 15
y7 (1+) 5.8 3.2 2.8 8.4 4.6 7.5 10.2 5.6 8 15 15 15
sum 4.6 2.8 2 6.3 4.2 7.1 7.8 5 7.4 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-1552 Collapse assay details

Data source: Panorama

Official Gene Symbol
HRAS
Peptide Sequence
SFEDIHQYR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
89
Peptide End
97
CPTAC ID
CPTAC-1552
Peptide Molecular Mass
1,193.5465
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM2


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 12.5 8.2 5.8 12.7 6.9 9.4 17.8 10.7 11 15 15 15
y5 (1+) 28.1 5.9 7.3 24.4 8.6 8.7 37.2 10.4 11.4 15 15 15
y4 (1+) 10.6 4.4 3 13.1 4.7 4.9 16.9 6.4 5.7 15 15 15
a2 (1+) 6 4.5 3.7 11.2 9.6 10.1 12.7 10.6 10.8 15 15 15
sum 3.7 3.7 2.9 9 7.5 8.3 9.7 8.4 8.8 15 15 15

Additional Resources and Comments