CBL, CPTAC-1567 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CBL	CBL2, RNF55

Sequence Length (AA)	Molecular Weight (Da)
906	99633

Protein Name
E3 ubiquitin-protein ligase CBL

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAGNVKKSSG	AGGGSGSGGS	GSGGLIGLMK	DAFQPHHHHH	HHLSPHPPGT
60	70	80	90	100
VDKKMVEKCW	KLMDKVVRLC	QNPKLALKNS	PPYILDLLPD	TYQHLRTILS
110	120	130	140	150
RYEGKMETLG	ENEYFRVFME	NLMKKTKQTI	SLFKEGKERM	YEENSQPRRN
160	170	180	190	200
LTKLSLIFSH	MLAELKGIFP	SGLFQGDTFR	ITKADAAEFW	RKAFGEKTIV
210	220	230	240	250
PWKSFRQALH	EVHPISSGLE	AMALKSTIDL	TCNDYISVFE	FDIFTRLFQP
260	270	280	290	300
WSSLLRNWNS	LAVTHPGYMA	FLTYDEVKAR	LQKFIHKPGS	YIFRLSCTRL
310	320	330	340	350
GQWAIGYVTA	DGNILQTIPH	NKPLFQALID	GFREGFYLFP	DGRNQNPDLT
360	370	380	390	400
GLCEPTPQDH	IKVTQEQYEL	YCEMGSTFQL	CKICAENDKD	VKIEPCGHLM
410	420	430	440	450
CTSCLTSWQE	SEGQGCPFCR	CEIKGTEPIV	VDPFDPRGSG	SLLRQGAEGA
460	470	480	490	500
PSPNYDDDDD	ERADDTLFMM	KELAGAKVER	PPSPFSMAPQ	ASLPPVPPRL
510	520	530	540	550
DLLPQRVCVP	SSASALGTAS	KAASGSLHKD	KPLPVPPTLR	DLPPPPPPDR
560	570	580	590	600
PYSVGAESRP	QRRPLPCTPG	DCPSRDKLPP	VPSSRLGDSW	LPRPIPKVPV
610	620	630	640	650
SAPSSSDPWT	GRELTNRHSL	PFSLPSQMEP	RPDVPRLGST	FSLDTSMSMN
660	670	680	690	700
SSPLVGPECD	HPKIKPSSSA	NAIYSLAARP	LPVPKLPPGE	QCEGEEDTEY
710	720	730	740	750
MTPSSRPLRP	LDTSQSSRAC	DCDQQIDSCT	YEAMYNIQSQ	APSITESSTF
760	770	780	790	800
GEGNLAAAHA	NTGPEESENE	DDGYDVPKPP	VPAVLARRTL	SDISNASSSF
810	820	830	840	850
GWLSLDGDPT	TNVTEGSQVP	ERPPKPFPRR	INSERKAGSC	QQGSGPAASA
860	870	880	890	900
ATASPQLSSE	IENLMSQGYS	YQDIQKALVI	AQNNIEMAKN	ILREFVSISS
906
PAHVAT

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for CPTAC-1567 Collapse assay details

Data source: Panorama

Official Gene Symbol: CBL
Peptide Sequence: GTEPIVVDPFDPR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 425
Peptide End: 437

CPTAC ID: CPTAC-1567
Peptide Molecular Mass: 1,440.7249
Species: Homo sapiens (Human)
Assay Type: Direct MRM or SRM
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Waters nanoACQUITY (Part Number 176016000)
Column Packing: Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions: 100 um x 100 mm
Flow Rate: 0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM2

CPTAC-1538: PIK3R2. ALGATFGPLLLR	CPTAC-1539: PIK3R1. FSAASSDNTENLIK	CPTAC-1540: PDPK1. QLLLTEGPHLYYVDPVNK
CPTAC-1541: MAPK1. VADPDHDHTGFLTEYVATR	CPTAC-1542: MAPK3. IADPEHDHTGFLTEYVATR	CPTAC-1543: MAP2K2. ISELGAGNGGVVTK
CPTAC-1544: MAP2K1. ISELGAGNGGVVFK	CPTAC-1545: MAP2K1. LIHLEIKPAIR	CPTAC-1546: RAF1. TISNGFGFK
CPTAC-1547: ARAF. TQHC[+57.0]DPEHFPFPAPANAPLQR	CPTAC-1548: RASA1. LLAITELLQQK	CPTAC-1549: NRAS. SFADINLYR
CPTAC-1550: KRAS. SFEDIHHYR	CPTAC-1551: HRAS. LVVVGAGGVGK	CPTAC-1552: HRAS. SFEDIHQYR
CPTAC-1553: SOS2. LPGYSSAEYR	CPTAC-1554: SOS1. LPSADVYR	CPTAC-1555: SOS1. EYIQPVQLR
CPTAC-1556: PTPN11. SNPGDFTLSVR	CPTAC-1557: GAB1. LTGDPDVLEYYK	CPTAC-1558: GRB2. ATADDELSFK
CPTAC-1559: SHC1. EAISLVC[+57.0]EAVPGAK	CPTAC-1560: EGFR. LTQLGTFEDHFLSLQR	CPTAC-1561: ADAM17. NIYLNSGLTSTK
CPTAC-1562: SHOC2. LDSLTTLYLR	CPTAC-1563: SPRED1. VPHQEENGC[+57.0]ADFFIR	CPTAC-1564: DUSP6. DSTNLDVLEEFGIK
CPTAC-1565: DUSP4. GSVSLEQILPAEEEVR	CPTAC-1566: ERRFI1. NSPSLFPC[+57.0]APLC[+57.0]ER	CPTAC-1567: CBL. GTEPIVVDPFDPR
CPTAC-1568: PEBP1. VLTPTQVK	CPTAC-1569: PTPRE. TGTFIALSNILER	CPTAC-1570: SPRY4. LQHPLTILPIDQVK

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	17.2	3.9	3.4	14.2	5.5	7.5	22.3	6.7	8.2	15	15	15
y5 (1+)	11.3	4.4	2.8	15	4.8	7.7	18.8	6.5	8.2	15	15	15
y2 (1+)	4.9	3.1	2.1	10.4	6.2	7	11.5	6.9	7.3	15	15	15
y10 (2+)	8	4.2	3.1	7.7	4.4	8.8	11.1	6.1	9.3	15	15	15
sum	4.6	3	2.1	7.6	5.2	7	8.9	6	7.3	15	15	15

CBL

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1567 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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CBL

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1567 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments