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Overview Data source: UniProt

Official Gene Symbol Other Aliases
MAP2K1 MEK1, PRKMK1
Sequence Length (AA) Molecular Weight (Da)
393 43439
Protein Name
Dual specificity mitogen-activated protein kinase kinase 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MPKKKPTPIQ LNPAPDGSAV NGTSSAETNL EALQKKLEEL ELDEQQRKRL
60 70 80 90 100
EAFLTQKQKV GELKDDDFEK ISELGAGNGG VVFKVSHKPS GLVMARKLIH
110 120 130 140 150
LEIKPAIRNQ IIRELQVLHE CNSPYIVGFY GAFYSDGEIS ICMEHMDGGS
160 170 180 190 200
LDQVLKKAGR IPEQILGKVS IAVIKGLTYL REKHKIMHRD VKPSNILVNS
210 220 230 240 250
RGEIKLCDFG VSGQLIDSMA NSFVGTRSYM SPERLQGTHY SVQSDIWSMG
260 270 280 290 300
LSLVEMAVGR YPIPPPDAKE LELMFGCQVE GDAAETPPRP RTPGRPLSSY
310 320 330 340 350
GMDSRPPMAI FELLDYIVNE PPPKLPSGVF SLEFQDFVNK CLIKNPAERA
360 370 380 390 393
DLKQLMVHAF IKRSDAEEVD FAGWLCSTIG LNQPSTPTHA AGV

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-5745 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP2K1
Peptide Sequence
ISELGAGNGGVVFK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
71
Peptide End
84
CPTAC ID
CPTAC-5745
Peptide Molecular Mass
1,346.7194
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM or SRM
Enrichment Method
N/A
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute
Submitting Lab PI
Amanda Paulovich, John Koomen, Steven A. Carr

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
SCIEX 5500 / Thermo Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Dionex Ultimate 3000
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM2


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 22.3 15.2 8.8 30.4 31.5 18.6 37.7 35 20.6 15 15 15
y9 (1+) 30.7 11.7 10.4 29.3 33.4 20.6 42.4 35.4 23.1 15 15 15
y10 (1+) 23.9 11.8 10.4 22.6 31.1 21.4 32.9 33.3 23.8 15 15 15
sum 23.3 12.6 9.8 25 31.5 20.4 34.2 33.9 22.6 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5411 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP2K1
Peptide Sequence
IPEQILGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
161
Peptide End
168
CPTAC ID
non-CPTAC-5411
Peptide Molecular Mass
896.5331
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
RSLCnano
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nl/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM2


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 11.6 7.6 2.4 14.6 7.7 3.1 18.6 10.8 3.9 15 15 15
y6 (1+) 15.9 3.5 1.4 17.9 3.5 3 23.9 4.9 3.3 15 15 15
y7 (1+) 7.4 2.8 1.1 9.5 3.7 1.9 12 4.6 2.2 15 15 15
sum 7.1 2.6 0.7 9.9 3.1 1.9 12.2 4 2 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5557 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP2K1
Peptide Sequence
ISELGAGNGGVVFK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
6
Peptide Start
71
Peptide End
84
CPTAC ID
non-CPTAC-5557
Peptide Molecular Mass
1,346.7194
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM or SRM
Enrichment Method
N/A
Matrix
Cell Line
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
SIS Peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
RSLCnano
Column Packing
C18 PepMap100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM2


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 23.4 9.6 7 33.1 32 17.2 40.5 33.4 18.6 14 14 14
y9 (1+) 31.2 7.2 6.1 29.7 34.5 16.9 43.1 35.2 18 14 14 14
y10 (1+) 24.3 7.4 6.3 24.7 31.5 17.7 34.6 32.4 18.8 14 14 14
y11 (1+) 22.4 6.1 7.4 29.8 31.7 18.5 37.3 32.3 19.9 14 14 14
sum 23.2 7.2 6.4 26.9 32 17.5 35.5 32.8 18.6 14 14 14


Additional Resources and Comments


Assay Details for non-CPTAC-5704 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP2K1
Peptide Modified Sequence
LC[+57.021464]DFGVSGQLIDS[+79.966331]MANSFVGTR
Modification Type
Phospho (ST)
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
206
Peptide End
227
CPTAC ID
non-CPTAC-5704
Peptide Molecular Mass
2,453.0756
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM or SRM
Enrichment Method
N/A
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Thermo Quantiva
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N R
LC
Dionex Ultimate 3000 RSLCnano
Column Packing
Pepmap 100
Column Dimensions
75um x 25cm
Flow Rate
300 nL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM2


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 6.3 3.6 3.7 5.2 4.1 5.1 8.2 5.5 6.3 15 15 15
y4 (1+) 11 7.3 4.3 4.4 8.8 6 11.8 11.4 7.4 15 15 15
y8 (1+) 14.1 8.9 7.2 13.4 7.9 7.1 19.5 11.9 10.1 15 15 15
y7 (1+) 13.7 6 5 13.2 4.6 6 19 7.6 7.8 15 15 15
sum 7.7 4.7 3.6 5.1 4.6 5 9.2 6.6 6.2 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5410 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP2K1
Peptide Sequence
VSHKPSGLVMAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
85
Peptide End
96
CPTAC ID
non-CPTAC-5410
Peptide Molecular Mass
1,280.7023
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
RSLCnano
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nl/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM2


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 4.5 3.8 2.2 5.8 3.9 3.6 7.3 5.4 4.2 15 15 15
y4 (1+) 3.6 3.6 1.8 5.5 3.9 3.5 6.6 5.3 3.9 15 15 15
y8 (1+) 4.4 2.1 2.4 6.2 3.7 3.2 7.6 4.3 4 15 15 15
y9 (1+) 11.6 4.9 6 11.3 5.8 6.1 16.2 7.6 8.6 15 15 15
sum 2.6 2.6 1.4 4.4 3.1 2.9 5.1 4 3.2 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-1544 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP2K1
Peptide Sequence
ISELGAGNGGVVFK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
71
Peptide End
84
CPTAC ID
CPTAC-1544
Peptide Molecular Mass
1,346.7194
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM2


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 10.8 3.7 3.2 14.6 5.1 7.2 18.2 6.3 7.9 15 15 15
y9 (1+) 24.5 6.2 4 28.8 6.8 5.1 37.8 9.2 6.5 15 15 15
y8 (1+) 18.3 5.1 3 18.3 5.6 6.7 25.9 7.6 7.3 15 15 15
b3 (1+) 18.6 4.3 3.4 11.9 4.5 7.4 22.1 6.2 8.1 15 15 15
sum 10.9 3.3 2.3 9.7 3.9 6.4 14.6 5.1 6.8 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-1545 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP2K1
Peptide Sequence
LIHLEIKPAIR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
98
Peptide End
108
CPTAC ID
CPTAC-1545
Peptide Molecular Mass
1,301.8183
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM2


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 40.1 7.8 20.7 40.6 13.4 24.5 57.1 15.5 32.1 15 15 15
y6 (1+) 39.6 14.4 16.4 45.3 13.4 17.7 60.2 19.7 24.1 15 15 15
y4 (1+) 17.1 5.1 8.5 19.6 12.2 11.7 26 13.2 14.5 15 15 15
y9 (2+) 16 14.5 9.4 22.6 10.6 15 27.7 18 17.7 15 15 15
sum 5 8.5 5.8 14.5 9.6 11.9 15.3 12.8 13.2 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-808 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP2K1
Peptide Sequence
QLMVHAFIK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
354
Peptide End
362
CPTAC ID
CPTAC-808
Peptide Molecular Mass
1,085.6056
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM2


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 7.4 7.4 6.4 5.7 7.5 7.4 9.3 10.5 9.8 15 15 15
y5 (1+) 13.8 6.6 3.7 13.7 6.2 4.5 19.4 9.1 5.8 15 15 15
y6 (1+) 12.8 11.2 10 16 12.3 10.2 20.5 16.6 14.3 15 15 15
sum 9.4 5.3 3.8 6.6 5.8 4.5 11.5 7.9 5.9 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-809 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP2K1
Peptide Sequence
VSHKPSGLVMAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
85
Peptide End
96
CPTAC ID
CPTAC-809
Peptide Molecular Mass
1,280.7023
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM2


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 7 6.8 5.2 6.7 8.9 4.7 9.7 11.2 7 15 15 15
y4 (1+) 11.6 7.3 3.8 12.5 5.9 6 17.1 9.4 7.1 15 15 15
y6 (1+) 21.7 17.7 8 22.5 13.2 8.1 31.3 22.1 11.4 15 15 15
y7 (1+) 12.5 17.3 10.1 12.7 14.1 10.6 17.8 22.3 14.6 15 15 15
y8 (1+) 8.8 6 3.7 8.5 6.6 4.7 12.2 8.9 6 15 15 15
y9 (1+) 14.9 15.2 8.3 24.4 14.3 9.9 28.6 20.9 12.9 15 15 15
sum 6.9 3.6 2.2 6.2 4 2.6 9.3 5.4 3.4 15 15 15


Additional Resources and Comments