SPRED1, CPTAC-1563 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
SPRED1	N/A

Sequence Length (AA)	Molecular Weight (Da)
444	50477

Protein Name
Sprouty-related, EVH1 domain-containing protein 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSEETATSDN	DNSYARVRAV	VMTRDDSSGG	WLPLGGSGLS	SVTVFKVPHQ
60	70	80	90	100
EENGCADFFI	RGERLRDKMV	VLECMLKKDL	IYNKVTPTFH	HWKIDDKKFG
110	120	130	140	150
LTFQSPADAR	AFDRGIRRAI	EDISQGCPES	KNEAEGADDL	QANEEDSSSS
160	170	180	190	200
LVKDHLFQQE	TVVTSEPYRS	SNIRPSPFED	LNARRVYMQS	QANQITFGQP
210	220	230	240	250
GLDIQSRSME	YVQRQISKEC	GSLKSQNRVP	LKSIRHVSFQ	DEDEIVRINP
260	270	280	290	300
RDILIRRYAD	YRHPDMWKND	LERDDADSSI	QFSKPDSKKS	DYLYSCGDET
310	320	330	340	350
KLSSPKDSVV	FKTQPSSLKI	KKSKRRKEDG	ERSRCVYCQE	RFNHEENVRG
360	370	380	390	400
KCQDAPDPIK	RCIYQVSCML	CAESMLYHCM	SDSEGDFSDP	CSCDTSDDKF
410	420	430	440	444
CLRWLALVAL	SFIVPCMCCY	VPLRMCHRCG	EACGCCGGKH	KAAG

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1563 Collapse assay details

Data source: Panorama

Official Gene Symbol: SPRED1
Peptide Modified Sequence: VPHQEENGC[+57.0]ADFFIR
Modification Type: Carbamidomethyl (C)
Protein - Site of Modification: 55
Peptide - Site of Modification: 9
Peptide Start: 47
Peptide End: 61

CPTAC ID: CPTAC-1563
Peptide Molecular Mass: 1,817.8155
Species: Homo sapiens (Human)
Assay Type: Direct MRM or SRM
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Waters nanoACQUITY (Part Number 176016000)
Column Packing: Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions: 100 um x 100 mm
Flow Rate: 0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM2

CPTAC-1538: PIK3R2. ALGATFGPLLLR	CPTAC-1539: PIK3R1. FSAASSDNTENLIK	CPTAC-1540: PDPK1. QLLLTEGPHLYYVDPVNK
CPTAC-1541: MAPK1. VADPDHDHTGFLTEYVATR	CPTAC-1542: MAPK3. IADPEHDHTGFLTEYVATR	CPTAC-1543: MAP2K2. ISELGAGNGGVVTK
CPTAC-1544: MAP2K1. ISELGAGNGGVVFK	CPTAC-1545: MAP2K1. LIHLEIKPAIR	CPTAC-1546: RAF1. TISNGFGFK
CPTAC-1547: ARAF. TQHC[+57.0]DPEHFPFPAPANAPLQR	CPTAC-1548: RASA1. LLAITELLQQK	CPTAC-1549: NRAS. SFADINLYR
CPTAC-1550: KRAS. SFEDIHHYR	CPTAC-1551: HRAS. LVVVGAGGVGK	CPTAC-1552: HRAS. SFEDIHQYR
CPTAC-1553: SOS2. LPGYSSAEYR	CPTAC-1554: SOS1. LPSADVYR	CPTAC-1555: SOS1. EYIQPVQLR
CPTAC-1556: PTPN11. SNPGDFTLSVR	CPTAC-1557: GAB1. LTGDPDVLEYYK	CPTAC-1558: GRB2. ATADDELSFK
CPTAC-1559: SHC1. EAISLVC[+57.0]EAVPGAK	CPTAC-1560: EGFR. LTQLGTFEDHFLSLQR	CPTAC-1561: ADAM17. NIYLNSGLTSTK
CPTAC-1562: SHOC2. LDSLTTLYLR	CPTAC-1563: SPRED1. VPHQEENGC[+57.0]ADFFIR	CPTAC-1564: DUSP6. DSTNLDVLEEFGIK
CPTAC-1565: DUSP4. GSVSLEQILPAEEEVR	CPTAC-1566: ERRFI1. NSPSLFPC[+57.0]APLC[+57.0]ER	CPTAC-1567: CBL. GTEPIVVDPFDPR
CPTAC-1568: PEBP1. VLTPTQVK	CPTAC-1569: PTPRE. TGTFIALSNILER	CPTAC-1570: SPRY4. LQHPLTILPIDQVK

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	14.5	6	5.3	15.2	9.4	7.6	21	11.2	9.3	15	15	15
y5 (1+)	11.3	3	2.8	11.2	6.7	6.6	15.9	7.3	7.2	15	15	15
y3 (1+)	12.2	4.6	5	19.8	8.3	8.6	23.3	9.5	9.9	15	15	15
sum	8.7	4	2.5	8.9	7.3	6.3	12.4	8.3	6.8	15	15	15

SPRED1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1563 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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SPRED1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1563 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments