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Overview Data source: UniProt

Official Gene Symbol Other Aliases
SPRED1 N/A
Sequence Length (AA) Molecular Weight (Da)
444 50477
Protein Name
Sprouty-related, EVH1 domain-containing protein 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSEETATSDN DNSYARVRAV VMTRDDSSGG WLPLGGSGLS SVTVFKVPHQ
60 70 80 90 100
EENGCADFFI RGERLRDKMV VLECMLKKDL IYNKVTPTFH HWKIDDKKFG
110 120 130 140 150
LTFQSPADAR AFDRGIRRAI EDISQGCPES KNEAEGADDL QANEEDSSSS
160 170 180 190 200
LVKDHLFQQE TVVTSEPYRS SNIRPSPFED LNARRVYMQS QANQITFGQP
210 220 230 240 250
GLDIQSRSME YVQRQISKEC GSLKSQNRVP LKSIRHVSFQ DEDEIVRINP
260 270 280 290 300
RDILIRRYAD YRHPDMWKND LERDDADSSI QFSKPDSKKS DYLYSCGDET
310 320 330 340 350
KLSSPKDSVV FKTQPSSLKI KKSKRRKEDG ERSRCVYCQE RFNHEENVRG
360 370 380 390 400
KCQDAPDPIK RCIYQVSCML CAESMLYHCM SDSEGDFSDP CSCDTSDDKF
410 420 430 440 444
CLRWLALVAL SFIVPCMCCY VPLRMCHRCG EACGCCGGKH KAAG

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1563 Collapse assay details

Data source: Panorama

Official Gene Symbol
SPRED1
Peptide Modified Sequence
VPHQEENGC[+57.0]ADFFIR
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
55
Peptide - Site of Modification
9
Peptide Start
47
Peptide End
61
CPTAC ID
CPTAC-1563
Peptide Molecular Mass
1,817.8155
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM2


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 14.5 6 5.3 15.2 9.4 7.6 21 11.2 9.3 15 15 15
y5 (1+) 11.3 3 2.8 11.2 6.7 6.6 15.9 7.3 7.2 15 15 15
y3 (1+) 12.2 4.6 5 19.8 8.3 8.6 23.3 9.5 9.9 15 15 15
sum 8.7 4 2.5 8.9 7.3 6.3 12.4 8.3 6.8 15 15 15

Additional Resources and Comments