SOS1, CPTAC-1555 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
SOS1	N/A

Sequence Length (AA)	Molecular Weight (Da)
1333	152464

Protein Name
Son of sevenless homolog 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MQAQQLPYEF	FSEENAPKWR	GLLVPALKKV	QGQVHPTLES	NDDALQYVEE
60	70	80	90	100
LILQLLNMLC	QAQPRSASDV	EERVQKSFPH	PIDKWAIADA	QSAIEKRKRR
110	120	130	140	150
NPLSLPVEKI	HPLLKEVLGY	KIDHQVSVYI	VAVLEYISAD	ILKLVGNYVR
160	170	180	190	200
NIRHYEITKQ	DIKVAMCADK	VLMDMFHQDV	EDINILSLTD	EEPSTSGEQT
210	220	230	240	250
YYDLVKAFMA	EIRQYIRELN	LIIKVFREPF	VSNSKLFSAN	DVENIFSRIV
260	270	280	290	300
DIHELSVKLL	GHIEDTVEMT	DEGSPHPLVG	SCFEDLAEEL	AFDPYESYAR
310	320	330	340	350
DILRPGFHDR	FLSQLSKPGA	ALYLQSIGEG	FKEAVQYVLP	RLLLAPVYHC
360	370	380	390	400
LHYFELLKQL	EEKSEDQEDK	ECLKQAITAL	LNVQSGMEKI	CSKSLAKRRL
410	420	430	440	450
SESACRFYSQ	QMKGKQLAIK	KMNEIQKNID	GWEGKDIGQC	CNEFIMEGTL
460	470	480	490	500
TRVGAKHERH	IFLFDGLMIC	CKSNHGQPRL	PGASNAEYRL	KEKFFMRKVQ
510	520	530	540	550
INDKDDTNEY	KHAFEIILKD	ENSVIFSAKS	AEEKNNWMAA	LISLQYRSTL
560	570	580	590	600
ERMLDVTMLQ	EEKEEQMRLP	SADVYRFAEP	DSEENIIFEE	NMQPKAGIPI
610	620	630	640	650
IKAGTVIKLI	ERLTYHMYAD	PNFVRTFLTT	YRSFCKPQEL	LSLIIERFEI
660	670	680	690	700
PEPEPTEADR	IAIENGDQPL	SAELKRFRKE	YIQPVQLRVL	NVCRHWVEHH
710	720	730	740	750
FYDFERDAYL	LQRMEEFIGT	VRGKAMKKWV	ESITKIIQRK	KIARDNGPGH
760	770	780	790	800
NITFQSSPPT	VEWHISRPGH	IETFDLLTLH	PIEIARQLTL	LESDLYRAVQ
810	820	830	840	850
PSELVGSVWT	KEDKEINSPN	LLKMIRHTTN	LTLWFEKCIV	ETENLEERVA
860	870	880	890	900
VVSRIIEILQ	VFQELNNFNG	VLEVVSAMNS	SPVYRLDHTF	EQIPSRQKKI
910	920	930	940	950
LEEAHELSED	HYKKYLAKLR	SINPPCVPFF	GIYLTNILKT	EEGNPEVLKR
960	970	980	990	1000
HGKELINFSK	RRKVAEITGE	IQQYQNQPYC	LRVESDIKRF	FENLNPMGNS
1010	1020	1030	1040	1050
MEKEFTDYLF	NKSLEIEPRN	PKPLPRFPKK	YSYPLKSPGV	RPSNPRPGTM
1060	1070	1080	1090	1100
RHPTPLQQEP	RKISYSRIPE	SETESTASAP	NSPRTPLTPP	PASGASSTTD
1110	1120	1130	1140	1150
VCSVFDSDHS	SPFHSSNDTV	FIQVTLPHGP	RSASVSSISL	TKGTDEVPVP
1160	1170	1180	1190	1200
PPVPPRRRPE	SAPAESSPSK	IMSKHLDSPP	AIPPRQPTSK	AYSPRYSISD
1210	1220	1230	1240	1250
RTSISDPPES	PPLLPPREPV	RTPDVFSSSP	LHLQPPPLGK	KSDHGNAFFP
1260	1270	1280	1290	1300
NSPSPFTPPP	PQTPSPHGTR	RHLPSPPLTQ	EVDLHSIAGP	PVPPRQSTSQ
1310	1320	1330	1333
HIPKLPPKTY	KREHTHPSMH	RDGPPLLENA	HSS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-1555 Collapse assay details

Data source: Panorama

Official Gene Symbol: SOS1
Peptide Sequence: EYIQPVQLR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 680
Peptide End: 688

CPTAC ID: CPTAC-1555
Peptide Molecular Mass: 1,144.6241
Species: Homo sapiens (Human)
Assay Type: Direct MRM or SRM
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Waters nanoACQUITY (Part Number 176016000)
Column Packing: Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions: 100 um x 100 mm
Flow Rate: 0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM2

CPTAC-1538: PIK3R2. ALGATFGPLLLR	CPTAC-1539: PIK3R1. FSAASSDNTENLIK	CPTAC-1540: PDPK1. QLLLTEGPHLYYVDPVNK
CPTAC-1541: MAPK1. VADPDHDHTGFLTEYVATR	CPTAC-1542: MAPK3. IADPEHDHTGFLTEYVATR	CPTAC-1543: MAP2K2. ISELGAGNGGVVTK
CPTAC-1544: MAP2K1. ISELGAGNGGVVFK	CPTAC-1545: MAP2K1. LIHLEIKPAIR	CPTAC-1546: RAF1. TISNGFGFK
CPTAC-1547: ARAF. TQHC[+57.0]DPEHFPFPAPANAPLQR	CPTAC-1548: RASA1. LLAITELLQQK	CPTAC-1549: NRAS. SFADINLYR
CPTAC-1550: KRAS. SFEDIHHYR	CPTAC-1551: HRAS. LVVVGAGGVGK	CPTAC-1552: HRAS. SFEDIHQYR
CPTAC-1553: SOS2. LPGYSSAEYR	CPTAC-1554: SOS1. LPSADVYR	CPTAC-1555: SOS1. EYIQPVQLR
CPTAC-1556: PTPN11. SNPGDFTLSVR	CPTAC-1557: GAB1. LTGDPDVLEYYK	CPTAC-1558: GRB2. ATADDELSFK
CPTAC-1559: SHC1. EAISLVC[+57.0]EAVPGAK	CPTAC-1560: EGFR. LTQLGTFEDHFLSLQR	CPTAC-1561: ADAM17. NIYLNSGLTSTK
CPTAC-1562: SHOC2. LDSLTTLYLR	CPTAC-1563: SPRED1. VPHQEENGC[+57.0]ADFFIR	CPTAC-1564: DUSP6. DSTNLDVLEEFGIK
CPTAC-1565: DUSP4. GSVSLEQILPAEEEVR	CPTAC-1566: ERRFI1. NSPSLFPC[+57.0]APLC[+57.0]ER	CPTAC-1567: CBL. GTEPIVVDPFDPR
CPTAC-1568: PEBP1. VLTPTQVK	CPTAC-1569: PTPRE. TGTFIALSNILER	CPTAC-1570: SPRY4. LQHPLTILPIDQVK

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	19.3	5.7	3.5	19.5	6	7.2	27.4	8.3	8	15	15	15
y6 (1+)	15.8	3.2	2.4	17.7	4.5	6.8	23.7	5.5	7.2	15	15	15
y5 (1+)	9.2	3.6	3.4	14.9	6.5	9.5	17.5	7.4	10.1	15	15	15
sum	8.8	3	2.9	13.1	5.7	8.3	15.8	6.4	8.8	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-1554 Collapse assay details

Data source: Panorama

Official Gene Symbol: SOS1
Peptide Sequence: LPSADVYR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 569
Peptide End: 576

CPTAC ID: CPTAC-1554
Peptide Molecular Mass: 919.4763
Species: Homo sapiens (Human)
Assay Type: Direct MRM or SRM
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu