SHOC2, CPTAC-1562 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
SHOC2	KIAA0862

Sequence Length (AA)	Molecular Weight (Da)
582	64888

Protein Name
Leucine-rich repeat protein SHOC-2

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSSSLGKEKD	SKEKDPKVPS	AKEREKEAKA	SGGFGKESKE	KEPKTKGKDA
60	70	80	90	100
KDGKKDSSAA	QPGVAFSVDN	TIKRPNPAPG	TRKKSSNAEV	IKELNKCREE
110	120	130	140	150
NSMRLDLSKR	SIHILPSSIK	ELTQLTELYL	YSNKLQSLPA	EVGCLVNLMT
160	170	180	190	200
LALSENSLTS	LPDSLDNLKK	LRMLDLRHNK	LREIPSVVYR	LDSLTTLYLR
210	220	230	240	250
FNRITTVEKD	IKNLSKLSML	SIRENKIKQL	PAEIGELCNL	ITLDVAHNQL
260	270	280	290	300
EHLPKEIGNC	TQITNLDLQH	NELLDLPDTI	GNLSSLSRLG	LRYNRLSAIP
310	320	330	340	350
RSLAKCSALE	ELNLENNNIS	TLPESLLSSL	VKLNSLTLAR	NCFQLYPVGG
360	370	380	390	400
PSQFSTIYSL	NMEHNRINKI	PFGIFSRAKV	LSKLNMKDNQ	LTSLPLDFGT
410	420	430	440	450
WTSMVELNLA	TNQLTKIPED	VSGLVSLEVL	ILSNNLLKKL	PHGLGNLRKL
460	470	480	490	500
RELDLEENKL	ESLPNEIAYL	KDLQKLVLTN	NQLTTLPRGI	GHLTNLTHLG
510	520	530	540	550
LGENLLTHLP	EEIGTLENLE	ELYLNDNPNL	HSLPFELALC	SKLSIMSIEN
560	570	580	582
CPLSHLPPQI	VAGGPSFIIQ	FLKMQGPYRA	MV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1562 Collapse assay details

Data source: Panorama

Official Gene Symbol: SHOC2
Peptide Sequence: LDSLTTLYLR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 191
Peptide End: 200

CPTAC ID: CPTAC-1562
Peptide Molecular Mass: 1,193.6656
Species: Homo sapiens (Human)
Assay Type: Direct MRM or SRM
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Waters nanoACQUITY (Part Number 176016000)
Column Packing: Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions: 100 um x 100 mm
Flow Rate: 0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM2

CPTAC-1538: PIK3R2. ALGATFGPLLLR	CPTAC-1539: PIK3R1. FSAASSDNTENLIK	CPTAC-1540: PDPK1. QLLLTEGPHLYYVDPVNK
CPTAC-1541: MAPK1. VADPDHDHTGFLTEYVATR	CPTAC-1542: MAPK3. IADPEHDHTGFLTEYVATR	CPTAC-1543: MAP2K2. ISELGAGNGGVVTK
CPTAC-1544: MAP2K1. ISELGAGNGGVVFK	CPTAC-1545: MAP2K1. LIHLEIKPAIR	CPTAC-1546: RAF1. TISNGFGFK
CPTAC-1547: ARAF. TQHC[+57.0]DPEHFPFPAPANAPLQR	CPTAC-1548: RASA1. LLAITELLQQK	CPTAC-1549: NRAS. SFADINLYR
CPTAC-1550: KRAS. SFEDIHHYR	CPTAC-1551: HRAS. LVVVGAGGVGK	CPTAC-1552: HRAS. SFEDIHQYR
CPTAC-1553: SOS2. LPGYSSAEYR	CPTAC-1554: SOS1. LPSADVYR	CPTAC-1555: SOS1. EYIQPVQLR
CPTAC-1556: PTPN11. SNPGDFTLSVR	CPTAC-1557: GAB1. LTGDPDVLEYYK	CPTAC-1558: GRB2. ATADDELSFK
CPTAC-1559: SHC1. EAISLVC[+57.0]EAVPGAK	CPTAC-1560: EGFR. LTQLGTFEDHFLSLQR	CPTAC-1561: ADAM17. NIYLNSGLTSTK
CPTAC-1562: SHOC2. LDSLTTLYLR	CPTAC-1563: SPRED1. VPHQEENGC[+57.0]ADFFIR	CPTAC-1564: DUSP6. DSTNLDVLEEFGIK
CPTAC-1565: DUSP4. GSVSLEQILPAEEEVR	CPTAC-1566: ERRFI1. NSPSLFPC[+57.0]APLC[+57.0]ER	CPTAC-1567: CBL. GTEPIVVDPFDPR
CPTAC-1568: PEBP1. VLTPTQVK	CPTAC-1569: PTPRE. TGTFIALSNILER	CPTAC-1570: SPRY4. LQHPLTILPIDQVK

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8 (1+)	26	6	11.8	30.1	NA	2.2	39.8	NA	12	15	3	3
y6 (1+)	14.6	4.7	3.6	24.1	3.8	9.2	28.2	6	9.9	15	15	15
y4 (1+)	16.2	5.8	4.8	17.9	7.8	9.3	24.1	9.7	10.5	15	15	15
sum	19.1	4.8	2.8	20	4.3	8.4	27.7	6.4	8.9	15	15	15

SHOC2

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1562 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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SHOC2

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1562 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments