GAB1, CPTAC-1557 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
GAB1	N/A

Sequence Length (AA)	Molecular Weight (Da)
694	76616

Protein Name
GRB2-associated-binding protein 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSGGEVVCSG	WLRKSPPEKK	LKRYAWKRRW	FVLRSGRLTG	DPDVLEYYKN
60	70	80	90	100
DHAKKPIRII	DLNLCQQVDA	GLTFNKKEFE	NSYIFDINTI	DRIFYLVADS
110	120	130	140	150
EEEMNKWVRC	ICDICGFNPT	EEDPVKPPGS	SLQAPADLPL	AINTAPPSTQ
160	170	180	190	200
ADSSSATLPP	PYQLINVPPH	LETLGIQEDP	QDYLLLINCQ	SKKPEPTRTH
210	220	230	240	250
ADSAKSTSSE	TDCNDNVPSH	KNPASSQSKH	GMNGFFQQQM	IYDSPPSRAP
260	270	280	290	300
SASVDSSLYN	LPRSYSHDVL	PKVSPSSTEA	DGELYVFNTP	SGTSSVETQM
310	320	330	340	350
RHVSISYDIP	PTPGNTYQIP	RTFPEGTLGQ	TSKLDTIPDI	PPPRPPKPHP
360	370	380	390	400
AHDRSPVETC	SIPRTASDTD	SSYCIPTAGM	SPSRSNTIST	VDLNKLRKDA
410	420	430	440	450
SSQDCYDIPR	AFPSDRSSSL	EGFHNHFKVK	NVLTVGSVSS	EELDENYVPM
460	470	480	490	500
NPNSPPRQHS	SSFTEPIQEA	NYVPMTPGTF	DFSSFGMQVP	PPAHMGFRSS
510	520	530	540	550
PKTPPRRPVP	VADCEPPPVD	RNLKPDRKVK	PAPLEIKPLP	EWEELQAPVR
560	570	580	590	600
SPITRSFARD	SSRFPMSPRP	DSVHSTTSSS	DSHDSEENYV	PMNPNLSSED
610	620	630	640	650
PNLFGSNSLD	GGSSPMIKPK	GDKQVEYLDL	DLDSGKSTPP	RKQKSSGSGS
660	670	680	690	694
SVADERVDYV	VVDQQKTLAL	KSTREAWTDG	RQSTESETPA	KSVK

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for CPTAC-1557 Collapse assay details

Data source: Panorama

Official Gene Symbol: GAB1
Peptide Sequence: LTGDPDVLEYYK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 38
Peptide End: 49

CPTAC ID: CPTAC-1557
Peptide Molecular Mass: 1,411.6871
Species: Homo sapiens (Human)
Assay Type: Direct MRM or SRM
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Waters nanoACQUITY (Part Number 176016000)
Column Packing: Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions: 100 um x 100 mm
Flow Rate: 0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM2

CPTAC-1538: PIK3R2. ALGATFGPLLLR	CPTAC-1539: PIK3R1. FSAASSDNTENLIK	CPTAC-1540: PDPK1. QLLLTEGPHLYYVDPVNK
CPTAC-1541: MAPK1. VADPDHDHTGFLTEYVATR	CPTAC-1542: MAPK3. IADPEHDHTGFLTEYVATR	CPTAC-1543: MAP2K2. ISELGAGNGGVVTK
CPTAC-1544: MAP2K1. ISELGAGNGGVVFK	CPTAC-1545: MAP2K1. LIHLEIKPAIR	CPTAC-1546: RAF1. TISNGFGFK
CPTAC-1547: ARAF. TQHC[+57.0]DPEHFPFPAPANAPLQR	CPTAC-1548: RASA1. LLAITELLQQK	CPTAC-1549: NRAS. SFADINLYR
CPTAC-1550: KRAS. SFEDIHHYR	CPTAC-1551: HRAS. LVVVGAGGVGK	CPTAC-1552: HRAS. SFEDIHQYR
CPTAC-1553: SOS2. LPGYSSAEYR	CPTAC-1554: SOS1. LPSADVYR	CPTAC-1555: SOS1. EYIQPVQLR
CPTAC-1556: PTPN11. SNPGDFTLSVR	CPTAC-1557: GAB1. LTGDPDVLEYYK	CPTAC-1558: GRB2. ATADDELSFK
CPTAC-1559: SHC1. EAISLVC[+57.0]EAVPGAK	CPTAC-1560: EGFR. LTQLGTFEDHFLSLQR	CPTAC-1561: ADAM17. NIYLNSGLTSTK
CPTAC-1562: SHOC2. LDSLTTLYLR	CPTAC-1563: SPRED1. VPHQEENGC[+57.0]ADFFIR	CPTAC-1564: DUSP6. DSTNLDVLEEFGIK
CPTAC-1565: DUSP4. GSVSLEQILPAEEEVR	CPTAC-1566: ERRFI1. NSPSLFPC[+57.0]APLC[+57.0]ER	CPTAC-1567: CBL. GTEPIVVDPFDPR
CPTAC-1568: PEBP1. VLTPTQVK	CPTAC-1569: PTPRE. TGTFIALSNILER	CPTAC-1570: SPRY4. LQHPLTILPIDQVK

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y10 (1+)	6.7	4.2	2.6	7.8	4.5	7.9	10.3	6.2	8.3	15	15	15
y8 (1+)	6.2	2.3	3.5	8.9	3.5	8.2	10.8	4.2	8.9	15	15	15
y5 (1+)	15	4.5	3.6	15.6	4.5	6.7	21.6	6.4	7.6	15	15	15
y8 (2+)	25.7	5.5	3.8	19.2	7.1	7.1	32.1	9	8.1	15	15	15
sum	4.9	2.9	2.4	7.5	4	7.1	9	4.9	7.5	15	15	15

GAB1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1557 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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GAB1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1557 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments