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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

ERRFI1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
ERRFI1 MIG6
Sequence Length (AA) Molecular Weight (Da)
462 50560
Protein Name
ERBB receptor feedback inhibitor 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSIAGVAAQE IRVPLKTGFL HNGRAMGNMR KTYWSSRSEF KNNFLNIDPI
60 70 80 90 100
TMAYSLNSSA QERLIPLGHA SKSAPMNGHC FAENGPSQKS SLPPLLIPPS
110 120 130 140 150
ENLGPHEEDQ VVCGFKKLTV NGVCASTPPL TPIKNSPSLF PCAPLCERGS
160 170 180 190 200
RPLPPLPISE ALSLDDTDCE VEFLTSSDTD FLLEDSTLSD FKYDVPGRRS
210 220 230 240 250
FRGCGQINYA YFDTPAVSAA DLSYVSDQNG GVPDPNPPPP QTHRRLRRSH
260 270 280 290 300
SGPAGSFNKP AIRISNCCIH RASPNSDEDK PEVPPRVPIP PRPVKPDYRR
310 320 330 340 350
WSAEVTSSTY SDEDRPPKVP PREPLSPSNS RTPSPKSLPS YLNGVMPPTQ
360 370 380 390 400
SFAPDPKYVS SKALQRQNSE GSASKVPCIL PIIENGKKVS STHYYLLPER
410 420 430 440 450
PPYLDKYEKF FREAEETNGG AQIQPLPADC GISSATEKPD SKTKMDLGGH
460 462
VKRKHLSYVV SP

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1566 Collapse assay details

Data source: Panorama

Official Gene Symbol
ERRFI1
Peptide Modified Sequence
NSPSLFPC[+57.0]APLC[+57.0]ER
Modification Type
Carbamidomethyl (C), Carbamidomethyl (C)
Protein - Site of Modification
142, 146
Peptide - Site of Modification
8, 12
Peptide Start
135
Peptide End
148
CPTAC ID
CPTAC-1566
Peptide Molecular Mass
1,646.7545
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM2

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 7.2 4.8 4.6 11.6 5.2 8.4 13.7 7.1 9.6 15 15 15
y8 (1+) 5.7 2.5 2.7 11.5 3.8 6.3 12.8 4.5 6.9 15 15 15
y12 (2+) 6.1 3 4.5 14.2 4.7 7.8 15.5 5.6 9 15 15 15
sum 4.4 2.7 3.2 11.7 3.7 6.9 12.5 4.6 7.6 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments