, CPTAC-3681 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
	N/A

Sequence Length (AA)	Molecular Weight (Da)
650	72783

Protein Name
Growth hormone receptor

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MDLCQVFLTL	ALAVTSSTFS	GSEATPATLG	KASPVLQRIN	PSLGTSSSGK
60	70	80	90	100
PRFTKCRSPE	LETFSCYWTE	GDNPDLKTPG	SIQLYYAKRE	SQRQAARIAH
110	120	130	140	150
EWTQEWKECP	DYVSAGKNSC	YFNSSYTSIW	IPYCIKLTTN	GDLLDQKCFT
160	170	180	190	200
VDEIVQPDPP	IGLNWTLLNI	SLTGIRGDIQ	VSWQPPPNAD	VLKGWIILEY
210	220	230	240	250
EIQYKEVNES	KWKVMGPIWL	TYCPVYSLRM	DKEHEVRVRS	RQRSFEKYSE
260	270	280	290	300
FSEVLRVIFP	QTNILEACEE	DIQFPWFLII	IFGIFGVAVM	LFVVIFSKQQ
310	320	330	340	350
RIKMLILPPV	PVPKIKGIDP	DLLKEGKLEE	VNTILGIHDN	YKPDFYNDDS
360	370	380	390	400
WVEFIELDID	EADVDEKTEG	SDTDRLLSND	HEKSAGILGA	KDDDSGRTSC
410	420	430	440	450
YDPDILDTDF	HTSDMCDGTL	KFRQSQKLNM	EADLLCLDQK	NLKNLPYDAS
460	470	480	490	500
LGSLHPSITQ	TVEENKPQPL	LSSETEATHQ	LASTPMSNPT	SLANIDFYAQ
510	520	530	540	550
VSDITPAGGD	VLSPGQKIKA	GIAQGNTQRE	VATPCQENYS	MNSAYFCESD
560	570	580	590	600
AKKCIAVARR	MEATSCIKPS	FNQEDIYITT	ESLTTTAQMS	ETADIAPDAE
610	620	630	640	650
MSVPDYTTVH	TVQSPRGLIL	NATALPLPDK	KNFPSSCGYV	STDQLNKIMQ

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3826 Collapse assay details

Data source: Panorama

Official Gene Symbol
Peptide Sequence: ALEWLGFIR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 44
Peptide End: 52

CPTAC ID: non-CPTAC-3826
Peptide Molecular Mass: 1,103.6128
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >80%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	7.2	3.6	4.1	11.4	14.2	17.6	13.5	14.6	18.1	15	15	15
y6 (1+)	9.4	5	3.6	11.4	14.9	17.3	14.8	15.7	17.7	15	15	15
y5 (1+)	10.6	4.1	4.9	12.2	16.2	18.7	16.2	16.7	19.3	15	15	15
y4 (1+)	4.4	2.8	3.8	7.3	15.2	17.2	8.5	15.5	17.6	15	15	15
b3 (1+)	7.5	2.2	4.8	10.9	15.1	16.6	13.2	15.3	17.3	15	15	15
sum	3.5	1.5	3.1	7	14.7	17.3	7.8	14.8	17.6	15	15	15

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	5.1	2	2.2	8.9	12.5	13.7	10.3	12.7	13.9	15	15	15
y5 (1+)	12.7	2.8	2.9	13.9	12.1	12.6	18.8	12.4	12.9	15	15	15
y4 (1+)	2.9	2.3	3.2	7.9	13.8	11.6	8.4	14	12	15	15	15
y6 (2+)	2.9	2.4	2.4	9	12.9	12.2	9.5	13.1	12.4	15	15	15
y4 (2+)	4.6	2.9	2.5	8.3	10.8	11.9	9.5	11.2	12.2	15	15	15
sum	2.1	1.4	1.7	8.3	12.7	12.1	8.6	12.8	12.2	15	15	15

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y11 (2+)	13	4.6	3	30.9	5.4	7.4	33.5	7.1	8	15	15	15
y7 (1+)	14.7	3.9	3.4	19.9	4.7	6.7	24.7	6.1	7.5	15	15	15
y9 (1+)	10.8	5.3	3.9	14	6.9	7.6	17.7	8.7	8.5	15	15	15
y11 (1+)	7.3	5.8	4.2	11.2	9.7	5.3	13.4	11.3	6.8	15	15	15
b2 (1+)	7.5	5.1	4.2	16.8	8.3	8.5	18.4	9.7	9.5	15	15	15
sum	4.3	2.1	2.1	11.4	6.5	5.9	12.2	6.8	6.3	15	15	15

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	13.3	7.7	5.8	20.2	8.9	8.6	24.2	11.8	10.4	15	15	15
y9 (1+)	12.5	7.2	8.1	15.6	7.9	13.8	20	10.7	16	15	15	15
y8 (1+)	14.9	10.3	6.7	20.7	10.2	15.1	25.5	14.5	16.5	15	15	15
y4 (1+)	11.5	7	7.5	15.8	8.5	9	19.5	11	11.7	15	15	15
b2 (1+)	11.9	11.8	10.2	14.1	11.7	10.3	18.5	16.6	14.5	15	15	15
sum	5.4	5.7	4.1	14	5.8	8.1	15	8.1	9.1	15	15	15

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	5.8	10.2	9.3	18.5	12.9	11.5	19.4	16.4	14.8	15	15	15
b3 (1+)	14.2	7.9	4.4	16.3	9.7	10.7	21.6	12.5	11.6	15	15	15
y3 (1+)	10.8	7.5	6.2	16.2	9.9	4.4	19.5	12.4	7.6	15	15	15
y5 (1+)	15.5	7.9	8.1	19	6.5	9.6	24.5	10.2	12.6	15	15	15
y6 (1+)	11.9	9.5	6.9	21.9	10	10.6	24.9	13.8	12.6	15	15	15
sum	5.4	5.2	4.5	16.3	7.4	7.7	17.2	9	8.9	15	15	15

Search form

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3826 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

Assay Details for non-CPTAC-3918 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

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Repeatability

Additional Resources and Comments

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Assay Details for non-CPTAC-3828 Collapse assay details

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Assay Parameters Collapse assay parameters

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Assay Details for non-CPTAC-3718 Collapse assay details

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Assay Parameters Collapse assay parameters

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Assay Details for non-CPTAC-3526 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

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Repeatability

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Assay Details for non-CPTAC-3837 Collapse assay details

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Assay Parameters Collapse assay parameters

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Repeatability

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Assay Details for non-CPTAC-3821 Collapse assay details

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Assay Parameters Collapse assay parameters

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Repeatability

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Assay Details for non-CPTAC-3550 Collapse assay details

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Assay Parameters Collapse assay parameters

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Repeatability

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Assay Details for non-CPTAC-3681 Collapse assay details

Assay Parameters Collapse assay parameters

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Repeatability

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Comments

Assay Details for non-CPTAC-3653 Collapse assay details

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Assay Parameters Collapse assay parameters

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