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Overview Data source: UniProt

Official Gene Symbol Other Aliases
N/A
Sequence Length (AA) Molecular Weight (Da)
113 12390
Protein Name
Ig kappa chain V-II region 17S29.1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
DIVMTQAVFS NPVTLGTSAS ISCRSSKSLL HSNGITYLYW YLQKPGQSPQ
60 70 80 90 100
LLLYQMSNLA SGVPDRFSSS GSGTDFTLRI SRVEAEDVGV YYCAHNLELP
110 113 113 113 113
YTFGGGTKLE IKR
113

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3826 Collapse assay details

Data source: Panorama

Official Gene Symbol
Peptide Sequence
ALEWLGFIR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
44
Peptide End
52
CPTAC ID
non-CPTAC-3826
Peptide Molecular Mass
1,103.6128
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 7.2 3.6 4.1 11.4 14.2 17.6 13.5 14.6 18.1 15 15 15
y6 (1+) 9.4 5 3.6 11.4 14.9 17.3 14.8 15.7 17.7 15 15 15
y5 (1+) 10.6 4.1 4.9 12.2 16.2 18.7 16.2 16.7 19.3 15 15 15
y4 (1+) 4.4 2.8 3.8 7.3 15.2 17.2 8.5 15.5 17.6 15 15 15
b3 (1+) 7.5 2.2 4.8 10.9 15.1 16.6 13.2 15.3 17.3 15 15 15
sum 3.5 1.5 3.1 7 14.7 17.3 7.8 14.8 17.6 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-3918 Collapse assay details

Data source: Panorama

Official Gene Symbol
Peptide Sequence
ALPAPIER
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
209
Peptide End
216
CPTAC ID
non-CPTAC-3918
Peptide Molecular Mass
865.5022
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 5.1 2 2.2 8.9 12.5 13.7 10.3 12.7 13.9 15 15 15
y5 (1+) 12.7 2.8 2.9 13.9 12.1 12.6 18.8 12.4 12.9 15 15 15
y4 (1+) 2.9 2.3 3.2 7.9 13.8 11.6 8.4 14 12 15 15 15
y6 (2+) 2.9 2.4 2.4 9 12.9 12.2 9.5 13.1 12.4 15 15 15
y4 (2+) 4.6 2.9 2.5 8.3 10.8 11.9 9.5 11.2 12.2 15 15 15
sum 2.1 1.4 1.7 8.3 12.7 12.1 8.6 12.8 12.2 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-3828 Collapse assay details

Data source: Panorama

Official Gene Symbol
Peptide Sequence
EPGEGATTYLVTSVLR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
275
Peptide End
290
CPTAC ID
non-CPTAC-3828
Peptide Molecular Mass
1,691.8730
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 15.2 3.7 3.8 18.8 9.7 6.7 24.2 10.4 7.7 15 15 15
y9 (1+) 24.2 7.5 1.9 23.7 9.8 7.3 33.9 12.3 7.5 15 15 15
y8 (1+) 8.8 3.5 3.9 12.8 5.9 7.3 15.5 6.9 8.3 15 15 15
y7 (1+) 20.5 7.2 2.7 19.5 13.2 5.8 28.3 15 6.4 15 15 15
y5 (1+) 14.4 8.2 3.3 15.7 10.9 6.9 21.3 13.6 7.6 15 15 15
sum 9.4 2.6 1.9 8.3 7.6 6.1 12.5 8 6.4 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-3718 Collapse assay details

Data source: Panorama

Official Gene Symbol
Peptide Sequence
FSGSGSGTDFTLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
67
Peptide End
79
CPTAC ID
non-CPTAC-3718
Peptide Molecular Mass
1,302.6092
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y11 (1+) 6.6 3.8 4.1 10.7 6.8 4.7 12.6 7.8 6.2 15 15 15
b2 (1+) 7.3 3.1 4.5 16.7 4.8 5.2 18.2 5.7 6.9 15 15 15
y11 (2+) 12 3.1 4.6 13 3.4 4.6 17.7 4.6 6.5 15 15 15
y7 (1+) 11.8 5.3 3.3 11.9 6.8 3.9 16.8 8.6 5.1 15 15 15
y9 (1+) 9.4 4.6 3.8 11.8 6.3 4.7 15.1 7.8 6 15 15 15
sum 2.5 1.8 2 7.4 3.7 2.6 7.8 4.1 3.3 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-3526 Collapse assay details

Data source: Panorama

Official Gene Symbol
Peptide Sequence
FSGSGSGTQFSLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
62
Peptide End
74
CPTAC ID
non-CPTAC-3526
Peptide Molecular Mass
1,301.6252
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y11 (2+) 13 4.6 3 30.9 5.4 7.4 33.5 7.1 8 15 15 15
y7 (1+) 14.7 3.9 3.4 19.9 4.7 6.7 24.7 6.1 7.5 15 15 15
y9 (1+) 10.8 5.3 3.9 14 6.9 7.6 17.7 8.7 8.5 15 15 15
y11 (1+) 7.3 5.8 4.2 11.2 9.7 5.3 13.4 11.3 6.8 15 15 15
b2 (1+) 7.5 5.1 4.2 16.8 8.3 8.5 18.4 9.7 9.5 15 15 15
sum 4.3 2.1 2.1 11.4 6.5 5.9 12.2 6.8 6.3 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-3837 Collapse assay details

Data source: Panorama

Official Gene Symbol
Peptide Sequence
FSSSGSGTDFTLR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
67
Peptide End
79
CPTAC ID
non-CPTAC-3837
Peptide Molecular Mass
1,360.6259
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 13.3 7.7 5.8 20.2 8.9 8.6 24.2 11.8 10.4 15 15 15
y9 (1+) 12.5 7.2 8.1 15.6 7.9 13.8 20 10.7 16 15 15 15
y8 (1+) 14.9 10.3 6.7 20.7 10.2 15.1 25.5 14.5 16.5 15 15 15
y4 (1+) 11.5 7 7.5 15.8 8.5 9 19.5 11 11.7 15 15 15
b2 (1+) 11.9 11.8 10.2 14.1 11.7 10.3 18.5 16.6 14.5 15 15 15
sum 5.4 5.7 4.1 14 5.8 8.1 15 8.1 9.1 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-3821 Collapse assay details

Data source: Panorama

Official Gene Symbol
Peptide Sequence
IHPSDSDTNYNQK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
70
Peptide End
82
CPTAC ID
non-CPTAC-3821
Peptide Molecular Mass
1,517.6747
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 4.6 6.2 8.9 14 9.9 10.7 14.7 11.7 13.9 15 15 15
b8 (2+) 17.5 8.1 8.1 22 11.4 8.9 28.1 14 12 15 15 15
b7 (1+) 6.1 6.9 7.3 12 12.7 11.1 13.5 14.5 13.3 15 15 15
y3 (1+) 4.3 6 8.2 9.7 10.8 11.6 10.6 12.4 14.2 15 15 15
y4 (1+) 3.9 3.6 7.2 8.6 8.7 9.9 9.4 9.4 12.2 15 15 15
sum 4.6 2.3 2.6 11.3 8.4 6 12.2 8.7 6.5 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-3550 Collapse assay details

Data source: Panorama

Official Gene Symbol
Peptide Sequence
LLIYDTSNLASGVPVR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
45
Peptide End
60
CPTAC ID
non-CPTAC-3550
Peptide Molecular Mass
1,716.9410
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 4.5 5.8 5.4 10.9 6.2 8.8 11.8 8.5 10.3 15 15 15
b10 (2+) 5 5 5.3 13.3 6.9 9.8 14.2 8.5 11.1 15 15 15
b2 (1+) 5.9 5.6 5.4 13 6.3 7.6 14.3 8.4 9.3 15 15 15
y3 (1+) 7 5.5 4.8 12.1 7 5.8 14 8.9 7.5 15 15 15
y5 (1+) 6.7 4.3 5.9 15.8 4.7 7.1 17.2 6.4 9.2 15 15 15
sum 2.5 1.6 2.3 11.9 4.1 6.6 12.2 4.4 7 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-3681 Collapse assay details

Data source: Panorama

Official Gene Symbol
Peptide Sequence
SNSQHPHQEIDNHLYHQLQR
Modification Type
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1
Peptide End
20
CPTAC ID
non-CPTAC-3681
Peptide Molecular Mass
2,480.1694
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 22.9 15 16.4 33.5 17.9 20.8 40.6 23.4 26.5 15 15 15
y18 (3+) 26 14.4 10.2 24.1 13.3 12.2 35.5 19.6 15.9 15 15 15
y4 (1+) 7.4 11.2 5.8 10.5 13 5.8 12.8 17.2 8.2 15 15 15
b13 (3+) 18.6 14.6 14.8 21.4 13.2 18.1 28.4 19.7 23.4 15 15 15
sum 7.5 7 4.4 12.1 7.2 6.3 14.2 10 7.7 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-3653 Collapse assay details

Data source: Panorama

Official Gene Symbol
Peptide Sequence
TLADGVPSR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
73
Peptide End
81
CPTAC ID
non-CPTAC-3653
Peptide Molecular Mass
914.4821
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 13.5 5.4 8 18.4 8.2 9.9 22.8 9.8 12.7 15 15 15
y7 (1+) 12.5 5.3 7.6 18.6 6 7.8 22.4 8 10.9 15 15 15
b2 (1+) 10.2 9 7.2 17.5 10.8 9.4 20.3 14.1 11.8 15 15 15
y3 (1+) 9.9 5.4 6.3 17.2 5.2 9.8 19.8 7.5 11.7 15 15 15
y5 (1+) 9.7 6.9 10.4 12.3 5.6 9.8 15.7 8.9 14.3 15 15 15
sum 3.6 3.6 4.6 13 3.8 6.9 13.5 5.2 8.3 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-3652 Collapse assay details

Data source: Panorama

Official Gene Symbol
Peptide Sequence
TSNLASGVPAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
74
Peptide End
84
CPTAC ID
non-CPTAC-3652
Peptide Molecular Mass
1,071.5673
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 5.8 10.2 9.3 18.5 12.9 11.5 19.4 16.4 14.8 15 15 15
b3 (1+) 14.2 7.9 4.4 16.3 9.7 10.7 21.6 12.5 11.6 15 15 15
y3 (1+) 10.8 7.5 6.2 16.2 9.9 4.4 19.5 12.4 7.6 15 15 15
y5 (1+) 15.5 7.9 8.1 19 6.5 9.6 24.5 10.2 12.6 15 15 15
y6 (1+) 11.9 9.5 6.9 21.9 10 10.6 24.9 13.8 12.6 15 15 15
sum 5.4 5.2 4.5 16.3 7.4 7.7 17.2 9 8.9 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-3575 Collapse assay details

Data source: Panorama

Official Gene Symbol
Peptide Sequence
VTYHSPGYVYGQFER
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
420
Peptide End
434
CPTAC ID
non-CPTAC-3575
Peptide Molecular Mass
1,801.8424
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b9 (2+) 14.5 11.1 10.5 15.5 11.4 12.1 21.2 15.9 16 15 15 15
b8 (1+) 13.1 6.3 7.4 19.1 9.1 6.5 23.2 11.1 9.8 15 15 15
y6 (2+) 20 12.5 6 33 9.5 10.6 38.6 15.7 12.2 15 15 15
y5 (1+) 11.2 10.3 8.5 13.5 11.5 7.8 17.5 15.4 11.5 15 15 15
y6 (1+) 11.2 8.5 6.7 14.9 12.4 7.2 18.6 15 9.8 15 15 15
sum 7.7 4.8 4.2 12.9 6 6 15 7.7 7.3 15 15 15

Additional Resources and Comments