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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Lrp1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Lrp1 A2mr
Sequence Length (AA) Molecular Weight (Da)
4545 504742
Protein Name
Prolow-density lipoprotein receptor-related protein 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MLTPPLLLLL PLLSALVSGA TMDAPKTCSP KQFACRDQIT CISKGWRCDG
60 70 80 90 100
ERDCPDGSDE APEICPQSKA QRCPPNEHSC LGTELCVPMS RLCNGIQDCM
110 120 130 140 150
DGSDEGAHCR ELRANCSRMG CQHHCVPTPS GPTCYCNSSF QLQADGKTCK
160 170 180 190 200
DFDECSVYGT CSQLCTNTDG SFTCGCVEGY LLQPDNRSCK AKNEPVDRPP
210 220 230 240 250
VLLIANSQNI LATYLSGAQV STITPTSTRQ TTAMDFSYAN ETVCWVHVGD
260 270 280 290 300
SAAQTQLKCA RMPGLKGFVD EHTINISLSL HHVEQMAIDW LTGNFYFVDD
310 320 330 340 350
IDDRIFVCNR NGDTCVTLLD LELYNPKGIA LDPAMGKVFF TDYGQIPKVE
360 370 380 390 400
RCDMDGQNRT KLVDSKIVFP HGITLDLVSR LVYWADAYLD YIEVVDYEGK
410 420 430 440 450
GRQTIIQGIL IEHLYGLTVF ENYLYATNSD NANTQQKTSV IRVNRFNSTE
460 470 480 490 500
YQVVTRVDKG GALHIYHQRR QPRVRSHACE NDQYGKPGGC SDICLLANSH
510 520 530 540 550
KARTCRCRSG FSLGSDGKSC KKPEHELFLV YGKGRPGIIR GMDMGAKVPD
560 570 580 590 600
EHMIPIENLM NPRALDFHAE TGFIYFADTT SYLIGRQKID GTERETILKD
610 620 630 640 650
GIHNVEGVAV DWMGDNLYWT DDGPKKTISV ARLEKAAQTR KTLIEGKMTH
660 670 680 690 700
PRAIVVDPLN GWMYWTDWEE DPKDSRRGRL ERAWMDGSHR DIFVTSKTVL
710 720 730 740 750
WPNGLSLDIP AGRLYWVDAF YDRIETILLN GTDRKIVYEG PELNHAFGLC
760 770 780 790 800
HHGNYLFWTE YRSGSVYRLE RGVAGAPPTV TLLRSERPPI FEIRMYDAQQ
810 820 830 840 850
QQVGTNKCRV NNGGCSSLCL ATPGSRQCAC AEDQVLDTDG VTCLANPSYV
860 870 880 890 900
PPPQCQPGEF ACANNRCIQE RWKCDGDNDC LDNSDEAPAL CHQHTCPSDR
910 920 930 940 950
FKCENNRCIP NRWLCDGDND CGNSEDESNA TCSARTCPPN QFSCASGRCI
960 970 980 990 1000
PISWTCDLDD DCGDRSDESA SCAYPTCFPL TQFTCNNGRC ININWRCDND
1010 1020 1030 1040 1050
NDCGDNSDEA GCSHSCSSTQ FKCNSGRCIP EHWTCDGDND CGDYSDETHA
1060 1070 1080 1090 1100
NCTNQATRPP GGCHSDEFQC RLDGLCIPLR WRCDGDTDCM DSSDEKSCEG
1110 1120 1130 1140 1150
VTHVCDPNVK FGCKDSARCI SKAWVCDGDS DCEDNSDEEN CEALACRPPS
1160 1170 1180 1190 1200
HPCANNTSVC LPPDKLCDGK DDCGDGSDEG ELCDQCSLNN GGCSHNCSVA
1210 1220 1230 1240 1250
PGEGIVCSCP LGMELGSDNH TCQIQSYCAK HLKCSQKCDQ NKFSVKCSCY
1260 1270 1280 1290 1300
EGWVLEPDGE SCRSLDPFKP FIIFSNRHEI RRIDLHKGDY SVLVPGLRNT
1310 1320 1330 1340 1350
IALDFHLSQS ALYWTDVVED KIYRGKLLDN GALTSFEVVI QYGLATPEGL
1360 1370 1380 1390 1400
AVDWIAGNIY WVESNLDQIE VAKLDGTLRT TLLAGDIEHP RAIALDPRDG
1410 1420 1430 1440 1450
ILFWTDWDAS LPRIEAASMS GAGRRTIHRE TGSGGWPNGL TVDYLEKRIL
1460 1470 1480 1490 1500
WIDARSDAIY SARYDGSGHM EVLRGHEFLS HPFAVTLYGG EVYWTDWRTN
1510 1520 1530 1540 1550
TLAKANKWTG HNVTVVQRTN TQPFDLQVYH PSRQPMAPNP CEANGGRGPC
1560 1570 1580 1590 1600
SHLCLINYNR TVSCACPHLM KLHKDNTTCY EFKKFLLYAR QMEIRGVDLD
1610 1620 1630 1640 1650
APYYNYIISF TVPDIDNVTV LDYDAREQRV YWSDVRTQAI KRAFINGTGV
1660 1670 1680 1690 1700
ETVVSADLPN AHGLAVDWVS RNLFWTSYDT NKKQINVARL DGSFKNAVVQ
1710 1720 1730 1740 1750
GLEQPHGLVV HPLRGKLYWT DGDNISMANM DGSNHTLLFS GQKGPVGLAI
1760 1770 1780 1790 1800
DFPESKLYWI SSGNHTINRC NLDGSELEVI DTMRSQLGKA TALAIMGDKL
1810 1820 1830 1840 1850
WWADQVSEKM GTCNKADGSG SVVLRNSTTL VMHMKVYDES IQLEHEGTNP
1860 1870 1880 1890 1900
CSVNNGDCSQ LCLPTSETTR SCMCTAGYSL RSGQQACEGV GSFLLYSVHE
1910 1920 1930 1940 1950
GIRGIPLDPN DKSDALVPVS GTSLAVGIDF HAENDTIYWV DMGLSTISRA
1960 1970 1980 1990 2000
KRDQTWREDV VTNGIGRVEG IAVDWIAGNI YWTDQGFDVI EVARLNGSFR
2010 2020 2030 2040 2050
YVVISQGLDK PRAITVHPEK GYLFWTEWGH YPRIERSRLD GTERVVLVNV
2060 2070 2080 2090 2100
SISWPNGISV DYQGGKLYWC DARMDKIERI DLETGENREV VLSSNNMDMF
2110 2120 2130 2140 2150
SVSVFEDFIY WSDRTHANGS IKRGCKDNAT DSVPLRTGIG VQLKDIKVFN
2160 2170 2180 2190 2200
RDRQKGTNVC AVANGGCQQL CLYRGGGQRA CACAHGMLAE DGASCREYAG
2210 2220 2230 2240 2250
YLLYSERTIL KSIHLSDERN LNAPVQPFED PEHMKNVIAL AFDYRAGTSP
2260 2270 2280 2290 2300
GTPNRIFFSD IHFGNIQQIN DDGSGRTTIV ENVGSVEGLA YHRGWDTLYW
2310 2320 2330 2340 2350
TSYTTSTITR HTVDQTRPGA FERETVITMS GDDHPRAFVL DECQNLMFWT
2360 2370 2380 2390 2400
NWNELHPSIM RAALSGANVL TLIEKDIRTP NGLAIDHRAE KLYFSDATLD
2410 2420 2430 2440 2450
KIERCEYDGS HRYVILKSEP VHPFGLAVYG EHIFWTDWVR RAVQRANKYV
2460 2470 2480 2490 2500
GSDMKLLRVD IPQQPMGIIA VANDTNSCEL SPCRINNGGC QDLCLLTHQG
2510 2520 2530 2540 2550
HVNCSCRGGR ILQEDFTCRA VNSSCRAQDE FECANGECIS FSLTCDGVSH
2560 2570 2580 2590 2600
CKDKSDEKPS YCNSRRCKKT FRQCNNGRCV SNMLWCNGVD DCGDGSDEIP
2610 2620 2630 2640 2650
CNKTACGVGE FRCRDGSCIG NSSRCNQFVD CEDASDEMNC SATDCSSYFR
2660 2670 2680 2690 2700
LGVKGVLFQP CERTSLCYAP SWVCDGANDC GDYSDERDCP GVKRPRCPLN
2710 2720 2730 2740 2750
YFACPSGRCI PMSWTCDKED DCENGEDETH CNKFCSEAQF ECQNHRCISK
2760 2770 2780 2790 2800
QWLCDGSDDC GDGSDEAAHC EGKTCGPSSF SCPGTHVCVP ERWLCDGDKD
2810 2820 2830 2840 2850
CTDGADESVT AGCLYNSTCD DREFMCQNRL CIPKHFVCDH DRDCADGSDE
2860 2870 2880 2890 2900
SPECEYPTCG PNEFRCANGR CLSSRQWECD GENDCHDHSD EAPKNPHCTS
2910 2920 2930 2940 2950
PEHKCNASSQ FLCSSGRCVA EALLCNGQDD CGDGSDERGC HVNECLSRKL
2960 2970 2980 2990 3000
SGCSQDCEDL KIGFKCRCRP GFRLKDDGRT CADLDECSTT FPCSQLCINT
3010 3020 3030 3040 3050
HGSYKCLCVE GYAPRGGDPH SCKAVTDEEP FLIFANRYYL RKLNLDGSNY
3060 3070 3080 3090 3100
TLLKQGLNNA VALDFDYREQ MIYWTDVTTQ GSMIRRMHLN GSNVQVLHRT
3110 3120 3130 3140 3150
GLSNPDGLAV DWVGGNLYWC DKGRDTIEVS KLNGAYRTVL VSSGLREPRA
3160 3170 3180 3190 3200
LVVDVQNGYL YWTDWGDHSL IGRIGMDGSG RSIIVDTKIT WPNGLTVDYV
3210 3220 3230 3240 3250
TERIYWADAR EDYIEFASLD GSNRHVVLSQ DIPHIFALTL FEDYVYWTDW
3260 3270 3280 3290 3300
ETKSINRAHK TTGANKTLLI STLHRPMDLH VFHALRQPDV PNHPCKVNNG
3310 3320 3330 3340 3350
GCSNLCLLSP GGGHKCACPT NFYLGGDGRT CVSNCTASQF VCKNDKCIPF
3360 3370 3380 3390 3400
WWKCDTEDDC GDHSDEPPDC PEFKCRPGQF QCSTGICTNP AFICDGDNDC
3410 3420 3430 3440 3450
QDNSDEANCD IHVCLPSQFK CTNTNRCIPG IFRCNGQDNC GDGEDERDCP
3460 3470 3480 3490 3500
EVTCAPNQFQ CSITKRCIPR VWVCDRDNDC VDGSDEPANC TQMTCGVDEF
3510 3520 3530 3540 3550
RCKDSGRCIP ARWKCDGEDD CGDGSDEPKE ECDERTCEPY QFRCKNNRCV
3560 3570 3580 3590 3600
PGRWQCDYDN DCGDNSDEES CTPRPCSESE FSCANGRCIA GRWKCDGDHD
3610 3620 3630 3640 3650
CADGSDEKDC TPRCDMDQFQ CKSGHCIPLR WRCDADADCM DGSDEEACGT
3660 3670 3680 3690 3700
GVRTCPLDEF QCNNTLCKPL AWKCDGEDDC GDNSDENPEE CARFICPPNR
3710 3720 3730 3740 3750
PFRCKNDRVC LWIGRQCDGV DNCGDGTDEE DCEPPTAQNP HCKDKKEFLC
3760 3770 3780 3790 3800
RNQRCLSSSL RCNMFDDCGD GSDEEDCSID PKLTSCATNA SMCGDEARCV
3810 3820 3830 3840 3850
RTEKAAYCAC RSGFHTVPGQ PGCQDINECL RFGTCSQLCN NTKGGHLCSC
3860 3870 3880 3890 3900
ARNFMKTHNT CKAEGSEYQV LYIADDNEIR SLFPGHPHSA YEQTFQGDES
3910 3920 3930 3940 3950
VRIDAMDVHV KAGRVYWTNW HTGTISYRSL PPAAPPTTSN RHRRQIDRGV
3960 3970 3980 3990 4000
THLNISGLKM PRGIAIDWVA GNVYWTDSGR DVIEVAQMKG ENRKTLISGM
4010 4020 4030 4040 4050
IDEPHAIVVD PLRGTMYWSD WGNHPKIETA AMDGTLRETL VQDNIQWPTG
4060 4070 4080 4090 4100
LAVDYHNERL YWADAKLSVI GSIRLNGTDP IVAADSKRGL SHPFSIDVFE
4110 4120 4130 4140 4150
DYIYGVTYIN NRVFKIHKFG HSPLINLTGG LSHASDVVLY HQHKQPEVTN
4160 4170 4180 4190 4200
PCDRKKCEWL CLLSPSGPVC TCPNGKRLDN GTCVPVPSPT PPPDAPRPGT
4210 4220 4230 4240 4250
CTLQCFNGGS CFLNARRQPK CRCQPRYTGD KCELDQCWEY CHNGGTCAAS
4260 4270 4280 4290 4300
PSGMPTCRCP TGFTGPKCTA QVCAGYCSNN STCTVNQGNQ PQCRCLPGFL
4310 4320 4330 4340 4350
GDRCQYRQCS GFCENFGTCQ MAADGSRQCR CTVYFEGPRC EVNKCSRCLQ
4360 4370 4380 4390 4400
GACVVNKQTG DVTCNCTDGR VAPSCLTCID HCSNGGSCTM NSKMMPECQC
4410 4420 4430 4440 4450
PPHMTGPRCE EQVVSQQQPG HMASILIPLL LLLLLLLVAG VVFWYKRRVR
4460 4470 4480 4490 4500
GAKGFQHQRM TNGAMNVEIG NPTYKMYEGG EPDDVGGLLD ADFALDPDKP
4510 4520 4530 4540 4545
TNFTNPVYAT LYMGGHGSRH SLASTDEKRE LLGRGPEDEI GDPLA

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-2223 Collapse assay details

Data source: Panorama

Official Gene Symbol
LRP1
Peptide Modified Sequence
DGTC[+57.0]IGN[+1.0]SSR
Modification Type
Carbamidomethyl (C), Deamidated (N)
Protein - Site of Modification
2617, 2620
Peptide - Site of Modification
4, 7
Peptide Start
2614
Peptide End
2623
CPTAC ID
CPTAC-2223
Peptide Molecular Mass
1,066.4349
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
LCMS-8040
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Nexera X2 UHPLC
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 19.4 13.1 6.2 20.7 13.7 5.9 28.4 19 8.6 15 15 15
y5 (1+) 8.8 5.1 3.9 9.9 4.7 3.5 13.2 6.9 5.2 15 15 15
y6 (1+) 19.2 8 4.7 19 10.6 5.6 27 13.3 7.3 15 15 15
sum 9.5 3.3 2.4 9.5 3.8 2.3 13.4 5 3.3 15 15 15

Additional Resources and Comments

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Assay Details for CPTAC-2222 Collapse assay details

Data source: Panorama

Official Gene Symbol
LRP1
Peptide Modified Sequence
ELQGN[+1.0]C[+57.0]SR
Modification Type
Deamidated (N), Carbamidomethyl (C)
Protein - Site of Modification
114, 115
Peptide - Site of Modification
5, 6
Peptide Start
110
Peptide End
117
CPTAC ID
CPTAC-2222
Peptide Molecular Mass
963.4080
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
LCMS-8040
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Nexera X2 UHPLC
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 38.6 21.4 18.3 39 20.2 21.1 54.9 29.4 27.9 15 15 15
y5 (1+) 22.7 10.6 10.6 16.8 15 8.2 28.2 18.4 13.4 15 15 15
y6 (1+) 24.7 22.1 35.4 31.4 19.7 37.6 40 29.6 51.6 15 15 15
sum 12.8 11 8.5 12.9 12.7 8.7 18.2 16.8 12.2 15 15 15

Additional Resources and Comments

Resources
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Assay Details for CPTAC-1617 Collapse assay details

Data source: Panorama

Official Gene Symbol
LRP1
Peptide Sequence
AALSGANVLTLIEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
2361
Peptide End
2374
CPTAC ID
CPTAC-1617
Peptide Molecular Mass
1,398.8082
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 26.1 9 8.9 32.6 13.5 10.8 41.8 16.2 14 15 15 15
y6 (1+) 10.4 3.9 2.7 20 5.6 8.1 22.5 6.8 8.5 15 15 15
y8 (1+) 12.7 3.9 2.7 21.8 4.8 8.4 25.2 6.2 8.8 15 15 15
sum 7.4 2.9 1.5 15.2 4.9 7.8 16.9 5.7 7.9 15 15 15

Additional Resources and Comments

Resources
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Assay Details for non-CPTAC-3838 Collapse assay details

Data source: Panorama

Official Gene Symbol
Lrp1
Peptide Sequence
TNTQPFDLQVYHPSR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1519
Peptide End
1533
CPTAC ID
non-CPTAC-3838
Peptide Molecular Mass
1,801.8747
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y11 (2+) 14.8 2.9 4.1 12.6 6.1 6.6 19.4 6.8 7.8 15 15 15
b2 (1+) 25.4 10.1 6.1 209.4 100.3 8.3 210.9 100.8 10.3 15 15 15
b3 (1+) 35.9 5.5 8.5 34.2 8.5 9.7 49.6 10.1 12.9 15 15 15
y3 (1+) 15.6 4.2 4.1 58.1 11.7 6.2 60.2 12.4 7.4 15 15 15
y13 (2+) 19 8.8 5.5 184.1 52 7.1 185.1 52.7 9 15 15 15
sum 11.1 3.3 2.6 164 26.4 5.8 164.4 26.6 6.4 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
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Comments